Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	990,592	T→C	5.4%	L158P (CTG→CCG) ‡	PP_0856 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	990,592	0	T	C	5.4%	222.2 / 2.0	75	L158P (CTG→CCG) ‡	PP_0856	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (38/32);  new base C (2/2);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGT  >  NC_002947/990458‑990736                                                                                                                                        |                                                                                                                                                   ccTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATg                                                                                                                                      >  3:763/1‑149 (MQ=255)  cTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTAGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         <  6:342702/145‑1 (MQ=255)  cTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTAGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         >  5:342702/1‑145 (MQ=255)     aaGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATgcc                                                                                                                                    <  5:7403/147‑1 (MQ=255)       gggTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCa                                                                                                                                <  5:16167/149‑1 (MQ=255)        ggTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCt                                                                                                                                           >  8:165528/1‑137 (MQ=255)        ggTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCt                                                                                                                                           <  7:165528/137‑1 (MQ=255)          tGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTg                                                                                                                             >  2:335483/1‑149 (MQ=255)                        tGGACTCCAGCACCGGTGAGGAGCGTTGGCGGCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTg                                                                                                                >  1:226146/1‑149 (MQ=255)                        tGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCAACCGCCGTTg                                                                                                                >  1:344132/1‑148 (MQ=255)                          gACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTgg                                                                                                               <  1:358031/147‑1 (MQ=255)                          gACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGa                                                                                                              <  2:226146/148‑1 (MQ=255)                          gACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCAACCGCCGTTg                                                                                                                <  2:344132/146‑1 (MQ=255)                            cTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCAGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATc                                                                                                            >  5:113975/1‑148 (MQ=255)                             tCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTa                                                                                                          >  2:266113/1‑149 (MQ=255)                               cAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGACAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTAc                                                                                                         <  2:127200/148‑1 (MQ=255)                                    ccGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAAc                                                                                                   <  2:79132/149‑1 (MQ=255)                                     cGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACt                                                                                                  >  2:191800/1‑149 (MQ=255)                                            gagCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                           >  5:356830/1‑149 (MQ=255)                                            gagCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggc                                                                                           >  7:262190/1‑149 (MQ=255)                                             agCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccggcc                                                                                          >  5:151394/1‑149 (MQ=255)                                               cGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGt                                                                                        >  2:153026/1‑149 (MQ=255)                                                    gc‑gcTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgc                                                                                               <  2:352788/137‑1 (MQ=255)                                                    gc‑gcTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgc                                                                                               >  1:352788/1‑137 (MQ=255)                                                       gcTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACg                                                                                 <  1:153026/149‑1 (MQ=255)                                                       gcTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACg                                                                                 >  2:273696/1‑149 (MQ=255)                                                         tCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGTGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCt                                                                               >  6:84210/1‑149 (MQ=255)                                                             gcgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGt                                                                           >  3:213684/1‑149 (MQ=255)                                                             gcgTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGt                                                                           <  1:335483/149‑1 (MQ=255)                                                                  aCCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         <  5:169186/82‑1 (MQ=37)                                                                  aCCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGCCGATCAGGCGATCGTCCTgg                                                                                                                                         >  6:169186/1‑82 (MQ=37)                                                                   ccAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccgg                                                                                            >  2:205275/1‑126 (MQ=255)                                                                   ccAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCgccgg                                                                                            <  1:205275/126‑1 (MQ=255)                                                                    cAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCg                                                                    <  4:213684/149‑1 (MQ=255)                                                                     aGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCgg                                                                   >  6:105018/1‑149 (MQ=255)                                                                          aGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGa                                                                                   <  2:217069/128‑1 (MQ=255)                                                                          aGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGa                                                                                   >  1:217069/1‑128 (MQ=255)                                                                                 gCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCg                                                          <  5:217834/144‑1 (MQ=255)                                                                                 gCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCg                                                          >  6:217834/1‑144 (MQ=255)                                                                                 gCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGc                                                     <  5:105018/149‑1 (MQ=255)                                                                                  ccccACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCCCCGGT‑‑GCGCCGATCGc                                                     >  3:70492/1‑148 (MQ=255)                                                                                    ccACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGccac                                                  >  6:294618/1‑149 (MQ=255)                                                                                       ccTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCaa                                               <  4:763/149‑1 (MQ=255)                                                                                             aacaacGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCt                                         >  2:369519/1‑149 (MQ=255)                                                                                              acaacGGTGACGTGGTGGAGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTg                                        >  7:20277/1‑149 (MQ=255)                                                                                                 acGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCg                                     <  6:356830/149‑1 (MQ=255)                                                                                                   ggTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGt                                   >  2:108952/1‑149 (MQ=255)                                                                                                     tGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTg                                 >  3:23820/1‑149 (MQ=255)                                                                                                       aCGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGc                                <  8:262190/148‑1 (MQ=255)                                                                                                            gtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCTCCGTTGGATCTACGAATACTCGCCGGCCGTGCTGACGGTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                          <  6:113975/149‑1 (MQ=255)                                                                                                            gtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCt                          >  8:85392/1‑149 (MQ=255)                                                                                                            gtggtggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGCCACCGGT‑‑GCACCGATCGCCACCAACCGCCTGGCGGTTACCGGCCt                          <  1:266113/149‑1 (MQ=255)                                                                                                                tggtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTc                       >  3:266481/1‑148 (MQ=255)                                                                                                                  gtgCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTAc                    >  8:293897/1‑149 (MQ=255)                                                                                                                    gCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCAGGCGGTTggtggc                            <  3:134369/139‑6 (MQ=255)                                                                                                                    gCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCAGGCGGTTggtggc                            >  4:134369/1‑134 (MQ=255)                                                                                                                    gCAGAAACAGGACGATCGCCTGATCGGCCTGGATGCAGCCACTGGCGAACGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCAACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCg                  >  8:304815/1‑149 (MQ=255)                                                                                                                        aCCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGCCCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGTGTGGCACCGGT‑‑gc                                                              >  4:29163/1‑101 (MQ=255)                                                                                                                        aCCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGTGTGGCACCGGT‑‑gc                                                              <  3:29163/101‑1 (MQ=255)                                                                                                                          ccAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAgg            >  8:155465/1‑149 (MQ=255)                                                                                                                          ccAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAgg            >  3:44274/1‑149 (MQ=255)                                                                                                                            aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCgg                 <  3:82941/142‑1 (MQ=255)                                                                                                                            aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCgg                 >  4:82941/1‑142 (MQ=255)                                                                                                                            aGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTa          <  1:369519/149‑1 (MQ=255)                                                                                                                              gACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCgg                             >  2:296965/1‑128 (MQ=255)                                                                                                                              gACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCgg                             <  1:296965/128‑1 (MQ=255)                                                                                                                              gACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGt        <  1:229805/149‑1 (MQ=255)                                                                                                                                cGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTgg      <  1:191800/149‑1 (MQ=255)                                                                                                                                   tCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGccac                                                  >  7:190108/1‑102 (MQ=255)                                                                                                                                   tCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGccac                                                  <  8:190108/102‑1 (MQ=255)                                                                                                                                   tCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCgg   >  6:263638/1‑149 (MQ=255)                                                                                                                                    cGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGGGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGt  <  4:229580/149‑1 (MQ=255)                                                                                                                                     gCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGTCCGCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCgg   <  1:108952/149‑1 (MQ=255)                                                                                                                                      ccTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCg                              >  1:294953/1‑119 (MQ=255)                                                                                                                                      ccTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCg                              <  2:294953/119‑1 (MQ=255)                                                                                                                                        tGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGt                        <  6:385014/123‑1 (MQ=255)                                                                                                                                        tGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGt                        >  5:385014/1‑123 (MQ=255)                                                                                                                                        |                                                                                                                                                   CCTCAAGGGTGAAGTCATTGCCCTGGACTCCAGCACCGGTGAGGAGCGTTGGC‑GCTCGCGCGTGACCAGCGAAGTGCTGGCCCCACCTGCCAACAACGGTGACGTGGTGGTGGTGCAGACCCAGGACGATCGCCTGATCGGCCTGGATGCCGCCACTGGCGACCGCCGTTGGATCTACGAAAACTCGCCGGCCGTGCTGACGCTGCGTGGCACCGGT‑‑GCGCCGATCGCCACCAACCGCCTGGCGGTTGCCGGCCTGTCTACCGGCAAGGTAGTGGCGGT  >  NC_002947/990458‑990736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑