Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,015,711 C→G 7.0% S177T (AGC→ACC)  PP_0875 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,015,7110CG 7.0% 176.0 / 2.6 55S177T (AGC→ACC) PP_0875hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/27);  new base G (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGATGGCCGGGGTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCTTTGCGGTAGTCGCA  >  NC_002947/1015568‑1015856
                                                                                                                                               |                                                                                                                                                  
aGTCGATGGCCGGGGTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTccc                                                                                                                                                >  4:219799/1‑148 (MQ=255)
  tCGATGGCCGGGGTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAg                                                                                                                                             <  3:105801/149‑1 (MQ=255)
    gATGGCCGGGGTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAgct                                                                                                                                           >  8:201610/1‑149 (MQ=255)
          cGGGGTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCAGCAGCTGGGGAGCCAgctgcgggt                                                                                                                                     <  8:318168/149‑5 (MQ=255)
            gggTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCCAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCgggg                                                                                                                                   <  3:204620/149‑1 (MQ=255)
            gggTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCgggg                                                                                                                                   >  1:195256/1‑149 (MQ=255)
            gggTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCgggg                                                                                                                                   <  3:11228/149‑1 (MQ=255)
                aTTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGGGAGCCAgctgcgg                                                                                                                                       >  3:71656/1‑139 (MQ=255)
                aTTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGGGAGCCAgctgcgg                                                                                                                                       <  4:71656/141‑3 (MQ=255)
                    ctgTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTc‑agcca                                                                                                                          <  7:317117/147‑1 (MQ=255)
                      gTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTc‑agccag                                                                                                                         <  7:235585/148‑1 (MQ=255)
                       tAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTATATGGCTCCAAACGCATTGATGACCCGCAGCTGGGTACCCAGCTGCTCGTGGCTc‑agcaa                                                                                                                          >  8:317117/1‑144 (MQ=255)
                       tAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCAGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCg                                                                                                                       >  3:333568/1‑149 (MQ=255)
                         gATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCgg                                                                                                                      <  2:297841/148‑1 (MQ=255)
                          aTCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTCTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTa                                                                                                                    <  1:120533/149‑1 (MQ=255)
                           tCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAg                                                                                                                   <  1:372026/149‑1 (MQ=255)
                              aTACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAgtcg                                                                                                                >  5:292132/1‑149 (MQ=255)
                               tACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAgtcgt                                                                                                               <  3:219799/149‑1 (MQ=255)
                                  gCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCt                                                                                                            >  6:326334/1‑149 (MQ=255)
                                     cAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCgg                                                                                                         >  5:267743/1‑149 (MQ=255)
                                         gcCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCAt                                                                                                     <  6:292132/149‑1 (MQ=255)
                                         gacTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCAt                                                                                                     <  6:267743/147‑1 (MQ=255)
                                           cTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAgct                                                                                                                                           >  8:132395/1‑110 (MQ=255)
                                            tCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCgg                                                                                                                      >  4:204440/1‑129 (MQ=255)
                                            tCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCgg                                                                                                                      <  3:204440/129‑1 (MQ=255)
                                                   cccGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTCAAGCCAGCGGTAGTCGTCCTCGGTCATGCCTTgg                                                                                              <  1:236946/147‑1 (MQ=255)
                                                   cccGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTgg                                                                                              <  4:124888/146‑1 (MQ=255)
                                                   cccGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTgg                                                                                              >  3:124888/1‑146 (MQ=255)
                                                    ccGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGgaga                                                                                           >  4:129256/1‑148 (MQ=255)
                                                                    aCCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCCGGGCTC‑AGCCAGACGTAGAAGTCCTCGGTCATGCCTTGGCCATACAAGTCGTCGAgg                                                                            >  5:305844/1‑147 (MQ=255)
                                                                                 gctggATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCt                                                              <  5:326334/148‑1 (MQ=255)
                                                                                  ctggATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGc                                                            <  4:333568/149‑1 (MQ=255)
                                                                                  ctggATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGc                                                            >  7:96298/1‑149 (MQ=255)
                                                                                      aTGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTc                                                        <  5:271159/149‑1 (MQ=255)
                                                                                       tGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTcc                                                       <  5:191371/149‑1 (MQ=255)
                                                                                           cGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCt                                                   >  3:269650/1‑149 (MQ=255)
                                                                                            gCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTc                                                  >  2:264983/1‑149 (MQ=255)
                                                                                                        cTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCggg                                              >  3:151865/1‑141 (MQ=255)
                                                                                                        cTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCggg                                              <  4:151865/141‑1 (MQ=255)
                                                                                                         tGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGcgcgcc                                     >  5:274308/1‑149 (MQ=255)
                                                                                                           tAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAg                                                                         >  6:19730/1‑111 (MQ=255)
                                                                                                                 gCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAAt                              <  8:96298/148‑1 (MQ=255)
                                                                                                                   tCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATa                             >  7:98620/1‑147 (MQ=255)
                                                                                                                          gCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGAcgcc                    <  2:195256/149‑1 (MQ=255)
                                                                                                                           cATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGAcgccg                   >  5:77459/1‑149 (MQ=255)
                                                                                                                            aTTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAg                                                                                     >  4:369399/1‑82 (MQ=255)
                                                                                                                            aTTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAg                                                                                     <  3:369399/82‑1 (MQ=255)
                                                                                                                            aTTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGAcgccgc                  >  3:163756/1‑149 (MQ=255)
                                                                                                                                        cAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCTTTGCGGTAGt      <  6:77459/149‑1 (MQ=255)
                                                                                                                                          gcgggCTCCCCCGCTGCGCGGGGCGC‑CCCCAGCGGGAGGCGTCCTCGGGCCTGCCCGGGCGATACACGTCGGCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCTTTGCGGTAGTCg    <  8:55523/146‑1 (MQ=255)
                                                                                                                                            tGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCt                >  6:250872/1‑135 (MQ=255)
                                                                                                                                            tGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCt                <  5:250872/135‑1 (MQ=255)
                                                                                                                                              gCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGAcgcc                    >  2:107352/1‑129 (MQ=255)
                                                                                                                                              gCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGAcgcc                    <  1:107352/129‑1 (MQ=255)
                                                                                                                                              gCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCTTTGCGGTAGTCGCa  <  6:274308/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
AGTCGATGGCCGGGGTATTTTCGTAGATCGATACGCCCAGCGCCTCCACTGCCCGGGCAAGGCCGCGCACCAGCTTGGCTGGCTGGATGGTCGCCGTGTGCGGGCTGTAAATGGCTCCAAACGCATTGCTGACCCGCAGCTGGCTCCCCAGCTGCTCGGGGCTC‑AGCCAGCGGTAGTCGTCCTCGGTCATGCCTTGGCGATACAAGTCGTCGAGGTAGGCGCGCAGGCTGCGTTCCTGCTCCGGGTAGCGCGCCGCGCAATAAAGGACGCCGCCTTTGCGGTAGTCGCA  >  NC_002947/1015568‑1015856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑