| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,086,497 | G→A | 10.1% | intergenic (+28/+30) | tldD → / ← PP_0941 | microcin B17 maturation protease/ribosome associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,086,497 | 0 | G | A | 10.1% | 163.1 / 7.3 | 59 | intergenic (+28/+30) | tldD/PP_0941 | microcin B17 maturation protease/ribosome associated protein |
| Reads supporting (aligned to +/- strand): ref base G (27/26); new base A (2/4); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
GACCTGGCACTGGACAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCACCAGCGAGCGT > NC_002947/1086350‑1086644 | gACCTGGCACTGGACAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCggg > 2:103936/1‑148 (MQ=255)gACCTGGCACTGGACAGCGGGGTAGGAACGGGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGc < 3:132090/149‑1 (MQ=255) gCACTGGACAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTcc > 8:99819/1‑149 (MQ=255) gACAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTccc < 7:99819/149‑1 (MQ=255) gACAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAACCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGACGTGCCCGCTcct < 2:202524/149‑2 (MQ=255) aCATCGGGGTAGCATCGTGCGGAAAGGACGGGCAGGCGGTTCCGGGCGGGGTGGGTCATGATACCTTGAACCTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATTGCTTCGGGGACCCTTGCAGGAGCGGGCACTTCCGCTCCca < 6:25562/149‑1 (MQ=255) aCAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCca < 3:273626/149‑1 (MQ=255) gCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCGGGAGCGGACGTGCCCGCTcctgcaa < 8:51203/149‑6 (MQ=255) gTAGGAACGTGCGGAAAGGACGGGCAGTCGGGGCCGGGCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGccc < 6:330084/149‑1 (MQ=255) tAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGATTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGcccc > 6:51266/1‑149 (MQ=255) tAGGAACGTGCGGAAAGGACGGGCAGGCGGTGCCGGGCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGGCCTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGccc < 1:103936/149‑1 (MQ=255) tCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGc > 6:320492/1‑148 (MQ=255) gTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACtcgtc > 7:44370/1‑147 (MQ=255) cGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAgcg > 8:30608/1‑124 (MQ=255) cGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAgcg < 7:30608/124‑1 (MQ=255) ggTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGAGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTTCAGGAGCGGGCACGTCCGCTCCCACAGCGCCCCAGTCCGTCAGCCCAACCCCCGCTGCAACTCGTCGAGTTcg > 7:339395/1‑149 (MQ=255) ggCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCaa < 4:198130/119‑1 (MQ=255) ggCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCaa > 3:198130/1‑119 (MQ=255) ggCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGAt > 1:44914/1‑149 (MQ=255) cTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACtt < 3:182360/149‑1 (MQ=255) cTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAac < 3:159081/149‑1 (MQ=255) ttGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAaca > 4:147271/1‑149 (MQ=255) gAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACAcc > 6:229919/1‑149 (MQ=255) aaaCTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACAcc < 1:94838/148‑1 (MQ=255) aaCTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCtt > 4:316768/1‑149 (MQ=255) aCTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCAc < 2:1422/68‑1 (MQ=255) gATGGGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCa < 6:272311/93‑1 (MQ=255) gATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTcgcg > 7:132769/1‑126 (MQ=255) gATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTcgcg < 8:132769/126‑1 (MQ=255) gATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTgcgcg > 2:124317/1‑149 (MQ=255) gATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCACACAGGGCCCCAGTCCGTCa > 5:272311/1‑93 (MQ=255) aTTAGCGTGTGCGGTACCGGGG‑ATTGTTGGATTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGAGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCgcggctg < 8:339395/149‑3 (MQ=255) aTTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTgc > 1:65938/1‑140 (MQ=255) aTTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTgc < 2:65938/140‑1 (MQ=255) cGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACtt < 8:282407/124‑1 (MQ=255) cGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACtt > 7:282407/1‑124 (MQ=255) gTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAggcg > 5:373840/1‑149 (MQ=255) cGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTg > 1:134217/1‑149 (MQ=255) cGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTcgc < 5:320492/149‑1 (MQ=255) cGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTcgc < 8:44370/149‑1 (MQ=255) cGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTcgc > 1:344127/1‑149 (MQ=255) ggg‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTgc < 1:145433/121‑1 (MQ=255) ggg‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTgc > 2:145433/1‑121 (MQ=255) ttCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTg > 4:15161/1‑149 (MQ=255) ggggACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGaa < 7:40675/98‑1 (MQ=255) ggggACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGaa > 8:40675/1‑98 (MQ=255) ggggACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTcgc > 8:5969/1‑132 (MQ=255) ggggACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTcgc < 7:5969/132‑1 (MQ=255) ggggACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGc < 6:32890/149‑1 (MQ=255) ttGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCa > 6:18211/1‑149 (MQ=255) ttGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCa > 7:93320/1‑149 (MQ=255) gCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCAc < 8:93320/148‑1 (MQ=255) tgggAGCGGACGTGCCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTgg > 2:198070/3‑136 (MQ=255) tgggAGCGGACGTGCCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTgg > 2:204191/3‑136 (MQ=255) tgggAGCGGACGTGCCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTgg < 1:204191/134‑1 (MQ=255) tgggAGCGGACGTGCCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCCGCTTGTTTCGCGCCTTCTCGTGCTgg < 1:198070/134‑1 (MQ=255) gAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTtc < 8:160657/124‑1 (MQ=255) gAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTtc > 7:160657/1‑124 (MQ=255) gAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCACCagc > 3:214191/1‑148 (MQ=255) cGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCACCagcgagc < 3:15161/149‑1 (MQ=255) ggCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCACCAGCGAGCGt < 5:229919/149‑1 (MQ=255) | GACCTGGCACTGGACAGCGGGGTAGGAACGTGCGGAAAGGACGGGCAGTCGGTGCCGGTCGGGGTGGGGCAGCCTACCTTGAAACTGGATGCGATTACCGTGGGCGGTACCGGGG‑CTTGATGGCTTCGGGGACCCTTGCAGGAGCGGGCACGTCCGCTCCCACAGGGCCCCAGTCCGTCAGCGCAACCCGCGCTGCAACTCGTCGAGGTCGCGGATGTACTTGAACACCTTGCGCGCGGCGGCAGGCGGCTTGTTTCGCGCCTTCTCGTGCTGGGCATGGCGCACCAGCGAGCGT > NC_002947/1086350‑1086644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |