Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,376	A→C	5.2%	L819R (CTG→CGG) ‡	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,376	0	A	C	5.2%	250.3 / 3.5	77	L819R (CTG→CGG) ‡	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (35/38);  new base C (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCC  >  NC_002947/1115230‑1115524                                                                                                                                                    |                                                                                                                                                      cGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcggcagc                                                                                                                                                     <  3:249435/149‑1 (MQ=255)  gACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcggcagcg                                                                                                                                                    <  5:82745/149‑1 (MQ=255)   aCCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcggcagcgg                                                                                                                                                   <  8:291271/149‑1 (MQ=255)         gCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGcc                                                                                                                                             <  1:211041/149‑1 (MQ=255)                 ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                     <  4:32868/149‑1 (MQ=255)                   aCAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGccgctgccgctg                                                                                                                                   <  5:352804/149‑1 (MQ=255)                    cAGCGGCGCTTCGTCGGCATCACCCCGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGt                                                                                                                                  <  5:99784/149‑1 (MQ=255)                       cggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCa                                                                                                                               >  6:69847/1‑149 (MQ=255)                        ggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAt                                                                                                                              <  2:104371/149‑1 (MQ=255)                        ggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑ACTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAt                                                                                                                              <  2:87261/149‑1 (MQ=255)                            cTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCg                                                                                                                          <  6:86616/149‑1 (MQ=255)                             ttCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGACAGCGGCTTGCCGGGGCCGATGTTCATTTCg‑g                                                                                                                        >  5:210424/1‑149 (MQ=255)                             atccgccgCATCCCCCACCACGCTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCg‑g                                                                                                                        <  8:318213/142‑1 (MQ=255)                                 tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACGGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑Gcgcg                                                                                                                    >  1:377269/1‑149 (MQ=255)                                 tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGt                                                                                                                                  >  2:267829/1‑136 (MQ=255)                                 tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGATGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGt                                                                                                                                  <  1:267829/136‑1 (MQ=255)                                    gCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGa                                                                                                                  >  3:332863/1‑148 (MQ=255)                                        cACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATAtt                                                                                                              >  1:274379/1‑149 (MQ=255)                                         aCCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGGCGGGGCCGATGTTCATTTCG‑GCGCGGATATTgc                                                                                                            >  2:23818/1‑149 (MQ=255)                                           ccAGTACGGTGAACGACTCGGCCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                                    >  1:252062/1‑124 (MQ=255)                                           ccAGTACGGTGAACGACTCGGCCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                                    <  2:252062/124‑1 (MQ=255)                                              gTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAgg                                                                                                       >  1:296390/1‑149 (MQ=255)                                                  ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGa                                                                                                   >  2:68181/1‑149 (MQ=255)                                                  ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGa                                                                                                   <  2:227001/149‑1 (MQ=255)                                                  ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGa                                                                                                   <  5:69847/149‑1 (MQ=255)                                                    tGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                >  8:37135/1‑119 (MQ=38)                                                    tGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGAc                                                                                                  >  6:345282/1‑148 (MQ=255)                                                        cGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATc                                                                                              >  1:28242/1‑148 (MQ=255)                                                          aCTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAAc                                                                                           >  2:230835/1‑149 (MQ=255)                                                           cTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑gcgc                                                                                                                     <  6:236997/122‑1 (MQ=255)                                                           cTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGACGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGCCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAAca                                                                                          >  5:30495/1‑148 (MQ=255)                                                             cGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCAt                                                                                               <  6:382461/142‑1 (MQ=255)                                                             cGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCAt                                                                                               >  5:382461/1‑142 (MQ=255)                                                              gACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAActct                                                                                        <  4:171421/148‑1 (MQ=255)                                                              gACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCtt                                                                                       >  3:201697/1‑149 (MQ=255)                                                                ccTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                     <  2:274379/149‑1 (MQ=255)                                                                ccTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                     <  1:230835/149‑1 (MQ=255)                                                                  ttGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAg                                                                                   >  4:192059/1‑149 (MQ=255)                                                                    ggCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGCGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                    <  1:68181/147‑1 (MQ=255)                                                                    ggCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATNTTGCGCAGGCCGACCATCAACTCTTTCAGc                                                                                  <  1:35384/148‑1 (MQ=255)                                                                    ggCCAGCTTATTCTGCAGC‑GCTTCGTTTTCCTGCTGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGTAACAGGGGCAGCGGCTTGCCGGGTCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCATCTCTTTCAGc                                                                                  <  6:30495/148‑1 (MQ=255)                                                                    cccgatcttcttCAGCAGC‑GCTTCGTTTTCCTACAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAg                                                                                   <  5:87373/141‑1 (MQ=255)                                                                      ccAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                     <  3:280902/143‑1 (MQ=255)                                                                      ccAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                     >  4:280902/1‑143 (MQ=255)                                                                       cAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACt                                                                              >  4:194718/1‑149 (MQ=255)                                                                        aGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTc                                                                             <  2:363895/149‑1 (MQ=255)                                                                            tcttcAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGTAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCg                                                                                                                          >  6:317977/1‑101 (MQ=255)                                                                            tcttcAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCg                                                                                                                          <  5:317977/101‑1 (MQ=255)                                                                             cttcAGCAGC‑GCTTCGTTTTCCTACAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAg                                                                                   >  6:87373/1‑138 (MQ=255)                                                                                  gcagc‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCt                                                                   >  2:368805/1‑149 (MQ=255)                                                                                     gc‑gcTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                <  4:332863/149‑1 (MQ=255)                                                                                            cGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgc                                                           <  1:190827/148‑1 (MQ=255)                                                                                            cGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgc                                                           <  2:28242/148‑1 (MQ=255)                                                                                               tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGCCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACACGATGTCGCCTTCGGCAgcggcg                                                       >  2:111077/1‑149 (MQ=255)                                                                                                 tCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGt                                                      <  4:201697/148‑1 (MQ=255)                                                                                                    tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGc                                                              <  1:80329/137‑1 (MQ=255)                                                                                                    tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGc                                                              >  2:80329/1‑137 (MQ=255)                                                                                                      cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCttt                                                                                      <  1:184230/111‑1 (MQ=255)                                                                                                      cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCttt                                                                                      >  2:184230/1‑111 (MQ=255)                                                                                                         gcggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTg                                             >  1:28312/1‑149 (MQ=255)                                                                                                                  gtcgtcGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGtt                                      <  5:345282/147‑1 (MQ=255)                                                                                                                   tcgtcgGCGTTGGCGTTCTTCAGGAACGGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGc                                   <  2:377269/149‑1 (MQ=255)                                                                                                                     gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGc                                   <  3:366257/147‑1 (MQ=255)                                                                                                                     gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGc                                   >  4:366257/1‑147 (MQ=255)                                                                                                                      tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCAc                                >  1:217543/1‑149 (MQ=255)                                                                                                                       cgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACt                               >  1:192743/1‑149 (MQ=255)                                                                                                                          cgttggcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCCCCTTCGGCAGCGCCGGCAATACGTGCTTCGTTGGCAACTgg                             >  6:204204/1‑148 (MQ=255)                                                                                                                             tggcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCAt                         >  4:26283/1‑149 (MQ=255)                                                                                                                               gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                       <  3:194718/149‑1 (MQ=255)                                                                                                                               gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                       >  3:105264/1‑149 (MQ=255)                                                                                                                               gcgttCTTCAGGAACAGCGACAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                       <  6:210424/149‑1 (MQ=255)                                                                                                                                cgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCAGTGGCCaacga                      <  1:5553/149‑5 (MQ=255)                                                                                                                                 gttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCt                     <  4:105264/149‑1 (MQ=255)                                                                                                                                      tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTc                                                     >  5:166294/1‑112 (MQ=255)                                                                                                                                      tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTc                                                     <  6:166294/112‑1 (MQ=255)                                                                                                                                       cAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcagca               >  6:263876/1‑149 (MQ=255)                                                                                                                                       cAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcagca               >  4:169262/1‑149 (MQ=255)                                                                                                                                         ggAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCatg             <  2:217543/149‑1 (MQ=255)                                                                                                                                          gAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCatga            >  8:280226/1‑149 (MQ=255)                                                                                                                                           aaCAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCatgat           <  3:169262/149‑1 (MQ=255)                                                                                                                                              agcggcagcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGt        <  1:23818/149‑1 (MQ=255)                                                                                                                                               gcggcagcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgca                  <  7:149767/138‑1 (MQ=255)                                                                                                                                               gcggcagcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgca                  >  8:149767/1‑138 (MQ=255)                                                                                                                                                cggcagcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATAc                                                >  6:65286/1‑107 (MQ=255)                                                                                                                                                cggcagcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATAc                                                <  5:65286/107‑1 (MQ=255)                                                                                                                                                cggcagcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCt      >  2:48698/1‑149 (MQ=255)                                                                                                                                                    agcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGcc  <  5:263876/149‑1 (MQ=255)                                                                                                                                                    agcggcTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGcc  >  7:114924/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                      CGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGC‑GCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCG‑GCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCC  >  NC_002947/1115230‑1115524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑