Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,135,613 T→G 8.5% E454D (GAA→GAC) ‡ PP_0997 ← sigma‑54 dependent transcriptional regulator/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,135,6130TG 8.5% 168.7 / 5.4 57E454D (GAA→GAC) ‡PP_0997sigma‑54 dependent transcriptional regulator/sensory box protein
Reads supporting (aligned to +/- strand):  ref base T (27/25);  new base G (2/3);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GTTATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGG  >  NC_002947/1135465‑1135759
                                                                                                                                                    |                                                                                                                                                  
gTTATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGt                                                                                                                                                    >  5:121951/1‑149 (MQ=255)
          cccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                           <  1:213076/148‑1 (MQ=255)
           ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTgg                                                                                                                                          <  1:265313/148‑1 (MQ=255)
            cGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                        <  2:103100/149‑4 (MQ=255)
             gggTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCACGGCCAGTTGCCGTGTTGACGGCTAGCTGGCGTATAAGCGCTGTAACAGTTCGAGCGCGAACTCACCTACAGCCTGTTCCAGGCTGGcc                                                                                                                                        >  6:204727/1‑148 (MQ=255)
              ggTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtaggt                                                                                                                                      <  3:318846/149‑6 (MQ=255)
                  ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                           <  6:342789/140‑1 (MQ=255)
                  ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                           >  5:342789/1‑140 (MQ=255)
                    ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGcc                                                                                                                                >  4:341826/1‑149 (MQ=255)
                      atatTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGAGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGt                                                                                                                              >  1:139902/1‑149 (MQ=255)
                              gCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcg                                                                                                                      >  3:345438/1‑149 (MQ=255)
                               cAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcgc                                                                                                                     <  3:86200/149‑1 (MQ=255)
                                 ggctctGCGCGATATCCGTGTGCGAAGGTTGCAAGCGCCCGGCCTTTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCATGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                   <  5:204727/147‑1 (MQ=255)
                                 gCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                   <  1:234910/149‑1 (MQ=255)
                                     ctGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCa                                                                                                               >  1:325963/1‑149 (MQ=255)
                                      tGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                         >  6:315534/1‑122 (MQ=255)
                                      tGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                         <  5:315534/122‑1 (MQ=255)
                                         gcgATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGa                                                                                                           <  6:128412/149‑1 (MQ=255)
                                         gcgATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGa                                                                                                           <  3:341826/149‑1 (MQ=255)
                                                     tgCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGat                                                                                               <  6:121951/149‑1 (MQ=255)
                                                        gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTGCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCTATATc                                                                                            <  5:239507/149‑1 (MQ=255)
                                                          aGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGGGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCGGTCTTGCCCTCGTGCCACCGAGGTGCCGGCGATATCCa                                                                                          <  8:360187/149‑1 (MQ=255)
                                                              tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGG‑CACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCg                                                                                                    <  4:375071/134‑1 (MQ=255)
                                                              tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGG‑CACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCg                                                                                                    >  3:375071/1‑134 (MQ=255)
                                                                 aaGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGcc                                                                                   <  5:384185/149‑1 (MQ=255)
                                                                 aaGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGcc                                                                                   <  2:10666/149‑1 (MQ=255)
                                                                  aGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCtt                                                                                                                           <  4:234843/108‑1 (MQ=255)
                                                                  aGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCtt                                                                                                                           >  3:234843/1‑108 (MQ=255)
                                                                     gcCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCTAGGTGCCGGCGATATCCAGGTCGCCGata                                                                               >  7:62371/1‑149 (MQ=255)
                                                                          gCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCTTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATccac                                                                          <  5:1314/149‑1 (MQ=255)
                                                                                   cGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGcc                                                                 >  2:159068/1‑149 (MQ=255)
                                                                                         gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCg                                                             >  3:166604/1‑147 (MQ=255)
                                                                                             ggTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATa                                                       <  2:325963/149‑1 (MQ=255)
                                                                                                  cTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgc                                                  >  4:361857/1‑149 (MQ=255)
                                                                                                   tGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcg                                                 >  3:78484/1‑149 (MQ=255)
                                                                                                       gTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgc                                             <  4:345438/149‑1 (MQ=255)
                                                                                                         ataAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcg                                            >  5:10763/1‑148 (MQ=255)
                                                                                                         ataAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcg                                            >  4:269825/1‑148 (MQ=255)
                                                                                                          taAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcgg                                           <  6:10763/148‑1 (MQ=255)
                                                                                                          taAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGa                                          <  1:111593/149‑1 (MQ=255)
                                                                                                               gcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGAt                                      >  4:317152/1‑148 (MQ=255)
                                                                                                                   tAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGt                                 >  2:378255/1‑149 (MQ=255)
                                                                                                                   tAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCATGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGt                                 >  7:181473/1‑149 (MQ=255)
                                                                                                                    aaCAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCt                                                               >  8:39896/1‑118 (MQ=255)
                                                                                                                    aaCAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCt                                                               <  7:39896/118‑1 (MQ=255)
                                                                                                                        gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGc                            <  1:159068/149‑1 (MQ=255)
                                                                                                                        gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCATGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGc                            <  8:181473/149‑1 (MQ=255)
                                                                                                                           cgcgCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTg                                                                   <  2:169386/107‑1 (MQ=255)
                                                                                                                           cgcgCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTg                                                                   >  1:169386/1‑107 (MQ=255)
                                                                                                                            gcgcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACt                        >  2:216327/1‑149 (MQ=255)
                                                                                                                              gcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTgg                      >  5:257235/1‑149 (MQ=255)
                                                                                                                              gcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTgg                      >  8:255842/1‑149 (MQ=255)
                                                                                                                                           gccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg         >  7:169680/3‑149 (MQ=255)
                                                                                                                                            ccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAg                                                          >  5:106817/2‑99 (MQ=255)
                                                                                                                                            ccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAg                                                          <  6:106817/98‑1 (MQ=255)
                                                                                                                                             cAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGc       >  5:238174/1‑149 (MQ=255)
                                                                                                                                                ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc      >  6:194913/1‑147 (MQ=255)
                                                                                                                                                ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc      >  8:9268/1‑147 (MQ=255)
                                                                                                                                                    ttCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg  <  7:9268/147‑1 (MQ=255)
                                                                                                                                                    ttCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg  <  6:257235/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGG  >  NC_002947/1135465‑1135759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑