Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,135,615 C→A 8.5% E454* (GAA→TAA) ‡ PP_0997 ← sigma‑54 dependent transcriptional regulator/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,135,6150CA 8.5% 185.9 / 6.9 59E454* (GAA→TAA) ‡PP_0997sigma‑54 dependent transcriptional regulator/sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base A (2/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

CCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGGTT  >  NC_002947/1135475‑1135762
                                                                                                                                            |                                                                                                                                                   
cccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                              <  1:213076/148‑1 (MQ=255)
 ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTgg                                                                                                                                             <  1:265313/148‑1 (MQ=255)
  cGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                           <  2:103100/149‑4 (MQ=255)
   gggTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCACGGCCAGTTGCCGTGTTGACGGCTAGCTGGCGTATAAGCGCTGTAACAGTTCGAGCGCGAACTCACCTACAGCCTGTTCCAGGCTGGcc                                                                                                                                           >  6:204727/1‑148 (MQ=255)
    ggTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtaggt                                                                                                                                         <  3:318846/149‑6 (MQ=255)
        ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                              <  6:342789/140‑1 (MQ=255)
        ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTg                                                                                                                                              >  5:342789/1‑140 (MQ=255)
          ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGcc                                                                                                                                   >  4:341826/1‑149 (MQ=255)
            atatTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGAGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGt                                                                                                                                 >  1:139902/1‑149 (MQ=255)
                    gCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcg                                                                                                                         >  3:345438/1‑149 (MQ=255)
                     cAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcgc                                                                                                                        <  3:86200/149‑1 (MQ=255)
                       ggctctGCGCGATATCCGTGTGCGAAGGTTGCAAGCGCCCGGCCTTTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCATGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                      <  5:204727/147‑1 (MQ=255)
                       gCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGt                                                                                                                      <  1:234910/149‑1 (MQ=255)
                           ctGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCa                                                                                                                  >  1:325963/1‑149 (MQ=255)
                            tGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                            >  6:315534/1‑122 (MQ=255)
                            tGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                            <  5:315534/122‑1 (MQ=255)
                               gcgATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGa                                                                                                              <  6:128412/149‑1 (MQ=255)
                               gcgATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGa                                                                                                              <  3:341826/149‑1 (MQ=255)
                                           tgCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGat                                                                                                  <  6:121951/149‑1 (MQ=255)
                                              gAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTGCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCTATATc                                                                                               <  5:239507/149‑1 (MQ=255)
                                                aGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGGGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCGGTCTTGCCCTCGTGCCACCGAGGTGCCGGCGATATCCa                                                                                             <  8:360187/149‑1 (MQ=255)
                                                    tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGG‑CACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCg                                                                                                       <  4:375071/134‑1 (MQ=255)
                                                    tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGG‑CACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCg                                                                                                       >  3:375071/1‑134 (MQ=255)
                                                       aaGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGcc                                                                                      <  5:384185/149‑1 (MQ=255)
                                                       aaGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGcc                                                                                      <  2:10666/149‑1 (MQ=255)
                                                        aGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCtt                                                                                                                              >  3:234843/1‑108 (MQ=255)
                                                        aGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCtt                                                                                                                              <  4:234843/108‑1 (MQ=255)
                                                           gcCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCTAGGTGCCGGCGATATCCAGGTCGCCGata                                                                                  >  7:62371/1‑149 (MQ=255)
                                                                gCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCTTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATccac                                                                             <  5:1314/149‑1 (MQ=255)
                                                                         cGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGcc                                                                    >  2:159068/1‑149 (MQ=255)
                                                                               gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCg                                                                >  3:166604/1‑147 (MQ=255)
                                                                                   ggTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATa                                                          <  2:325963/149‑1 (MQ=255)
                                                                                        cTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgc                                                     >  4:361857/1‑149 (MQ=255)
                                                                                         tGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcg                                                    >  3:78484/1‑149 (MQ=255)
                                                                                             gTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgc                                                <  4:345438/149‑1 (MQ=255)
                                                                                               ataAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcg                                               >  4:269825/1‑148 (MQ=255)
                                                                                               ataAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcg                                               >  5:10763/1‑148 (MQ=255)
                                                                                                taAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcgcgg                                              <  6:10763/148‑1 (MQ=255)
                                                                                                taAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGa                                             <  1:111593/149‑1 (MQ=255)
                                                                                                     gcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGAt                                         >  4:317152/1‑148 (MQ=255)
                                                                                                         tAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGt                                    >  2:378255/1‑149 (MQ=255)
                                                                                                         tAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCATGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGt                                    >  7:181473/1‑149 (MQ=255)
                                                                                                          aaCAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCt                                                                  >  8:39896/1‑118 (MQ=255)
                                                                                                          aaCAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCt                                                                  <  7:39896/118‑1 (MQ=255)
                                                                                                              gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGc                               <  1:159068/149‑1 (MQ=255)
                                                                                                              gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCATGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGc                               <  8:181473/149‑1 (MQ=255)
                                                                                                                 cgcgCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTg                                                                      <  2:169386/107‑1 (MQ=255)
                                                                                                                 cgcgCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTg                                                                      >  1:169386/1‑107 (MQ=255)
                                                                                                                  gcgcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACt                           >  2:216327/1‑149 (MQ=255)
                                                                                                                    gcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTgg                         >  5:257235/1‑149 (MQ=255)
                                                                                                                    gcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTgg                         >  8:255842/1‑149 (MQ=255)
                                                                                                                                 gccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg            >  7:169680/3‑149 (MQ=255)
                                                                                                                                  ccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAg                                                             >  5:106817/2‑99 (MQ=255)
                                                                                                                                  ccAGCCTGGAACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAg                                                             <  6:106817/98‑1 (MQ=255)
                                                                                                                                   cAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGc          >  5:238174/1‑149 (MQ=255)
                                                                                                                                      ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc         >  6:194913/1‑147 (MQ=255)
                                                                                                                                      ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc         >  8:9268/1‑147 (MQ=255)
                                                                                                                                          ttCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg     <  6:257235/147‑1 (MQ=255)
                                                                                                                                          ttCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg     <  7:9268/147‑1 (MQ=255)
                                                                                                                                            ccAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGGtt  <  4:78484/148‑1 (MQ=255)
                                                                                                                                            ccAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGGtt  <  2:139902/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCGCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGGTT  >  NC_002947/1135475‑1135762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑