Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 150,829 G→T 5.9% L41M (CTG→ATG) ‡ PP_0142 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947150,8290GT 5.9% 249.1 / 3.8 77L41M (CTG→ATG) ‡PP_0142ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (33/39);  new base T (2/3);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AGCGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTA  >  NC_002947/150681‑150974
                                                                                                                                                    |                                                                                                                                                 
agcGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAg                                                                                                                                                   >  5:128078/1‑149 (MQ=255)
  cgcCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCACAGgc                                                                                                                                                 <  4:18439/149‑1 (MQ=255)
    ccTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGgcgc                                                                                                                                               >  5:143015/1‑149 (MQ=255)
     ctggctggCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGcgct                                                                                                                                              <  8:229206/149‑1 (MQ=255)
        gctggctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGcgctcgc                                                                                                                                           <  8:194577/149‑1 (MQ=255)
          tggctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCgctcgctc                                                                                                                                         >  5:347501/1‑149 (MQ=255)
          tggctTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCgctcgctc                                                                                                                                         >  6:6274/1‑149 (MQ=255)
                 aGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCt                                                                                                                                  >  2:290806/1‑149 (MQ=255)
                   gCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGGTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCACGCGCtt                                                                                                                                 >  6:376511/1‑148 (MQ=255)
                    cAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGa                                                                                                                               <  2:232259/149‑1 (MQ=255)
                     aGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAg                                                                                                                              <  3:343312/149‑1 (MQ=255)
                     aGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAg                                                                                                                              >  2:114449/1‑149 (MQ=255)
                     aGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTACGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAg                                                                                                                              >  7:339764/1‑149 (MQ=255)
                       cgcgTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGt                                                                                                                            >  4:267422/1‑149 (MQ=255)
                        gcgTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGtt                                                                                                                           <  4:108418/149‑1 (MQ=255)
                        gcgTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGtt                                                                                                                           <  4:113903/149‑1 (MQ=255)
                        gcgTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTACGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGtt                                                                                                                           <  8:339764/149‑1 (MQ=255)
                              gTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTa                                                                                                                     <  7:275443/149‑1 (MQ=255)
                                        aCAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAgg                                                                                                           <  6:143015/149‑1 (MQ=255)
                                        aCAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAgg                                                                                                           <  3:267422/149‑1 (MQ=255)
                                        aCAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAgg                                                                                                           <  8:89517/149‑1 (MQ=255)
                                         cAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAggg                                                                                                          <  1:290806/149‑1 (MQ=255)
                                             cgcTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCa                                                                                                      <  5:27010/149‑1 (MQ=255)
                                                   gAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAcc                                                                                                <  6:128078/149‑1 (MQ=255)
                                                   gAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAcc                                                                                                <  7:325705/149‑1 (MQ=255)
                                                     gCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCgg                                                                                              <  6:326327/149‑1 (MQ=255)
                                                      ccTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCgg                                                                                              >  2:224151/1‑148 (MQ=255)
                                                          gCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAAt                                                                                          <  1:114449/148‑1 (MQ=255)
                                                              ttCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgca                                                                                     <  5:6274/149‑1 (MQ=255)
                                                               tCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGcgct                                                                                                                                              <  1:132587/91‑1 (MQ=255)
                                                               tCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGcgct                                                                                                                                              >  2:132587/1‑91 (MQ=255)
                                                                cGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcag                                                                                    <  7:78257/148‑1 (MQ=255)
                                                                      gcagcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGcc                                                                             >  7:32678/1‑149 (MQ=255)
                                                                       cagcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCg                                                                            <  1:224151/149‑1 (MQ=255)
                                                                        agcGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCgg                                                                           >  8:354854/1‑149 (MQ=255)
                                                                            aaGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                              >  2:199480/1‑142 (MQ=255)
                                                                            aaGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                              <  1:199480/142‑1 (MQ=255)
                                                                            aaGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTgg                                                                       >  2:86859/1‑149 (MQ=255)
                                                                                 ccGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcaggcag                                                                                <  8:322903/135‑1 (MQ=255)
                                                                                 ccGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACgcaggcag                                                                                >  7:322903/1‑135 (MQ=255)
                                                                                 ccGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgctgct                                                                  >  6:245884/1‑149 (MQ=255)
                                                                                 ccGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgctgct                                                                  >  3:60620/1‑149 (MQ=255)
                                                                                 ccGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGgctgct                                                                  >  3:90609/1‑149 (MQ=255)
                                                                                 ccGGCAGTGTCAAGGCGGACCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGAGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGGTGgctgat                                                                  >  3:85482/1‑147 (MQ=255)
                                                                                  cGGCAGTGTCCAGGCGGGCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCGCGCTCTCGCGCTTGAGTTTGGCGTAGGGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGcttctg                                                                 <  6:305499/149‑1 (MQ=255)
                                                                                  cGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCg                                                                                                                       <  4:71079/95‑1 (MQ=255)
                                                                                  cGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCg                                                                                                                       >  3:71079/1‑95 (MQ=255)
                                                                                          tCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                              <  8:294277/128‑1 (MQ=255)
                                                                                          tCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCaggcaggc                                                                              >  7:294277/1‑128 (MQ=255)
                                                                                              ggcggcCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTg                                                     >  7:193352/1‑149 (MQ=255)
                                                                                              ggcggcCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTg                                                     >  1:170261/1‑149 (MQ=255)
                                                                                                  gcCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGgcgc                                                 >  1:285810/1‑149 (MQ=255)
                                                                                                  gcCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCACGCTCTAGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTAACCGGTAATACGCAGGCAGGCCGGTTGGCTGCAGGTGTCCAGGGTGgccc                                                 >  7:58184/1‑147 (MQ=255)
                                                                                                              gAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCa                                     >  2:274866/1‑149 (MQ=255)
                                                                                                               aGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCAt                                    <  5:245884/149‑1 (MQ=255)
                                                                                                                ggTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATa                                   <  1:86859/149‑1 (MQ=255)
                                                                                                                 gTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAc                                                                                                 <  2:228629/86‑1 (MQ=255)
                                                                                                                 gTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCAc                                                                                                 >  1:228629/1‑86 (MQ=255)
                                                                                                                    ggCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCt                               >  4:321595/1‑149 (MQ=255)
                                                                                                                     gCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTc                              <  8:32678/149‑1 (MQ=255)
                                                                                                                      cGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCATGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTc                              >  8:349033/1‑148 (MQ=255)
                                                                                                                        aCGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCAt                           <  6:347501/149‑1 (MQ=255)
                                                                                                                          ggTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGGAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCTCTGGGGTTGGGCATAGGCTCCATGc                         <  2:285810/149‑1 (MQ=255)
                                                                                                                           gtgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCa                                                                                                      <  1:333622/71‑1 (MQ=255)
                                                                                                                           gtgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCa                                                                                                      >  2:333622/1‑71 (MQ=255)
                                                                                                                            tgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAAt                                                                                          >  8:173541/1‑82 (MQ=255)
                                                                                                                            tgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAAt                                                                                          <  7:173541/82‑1 (MQ=255)
                                                                                                                            tgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCaa                       <  7:354854/149‑1 (MQ=255)
                                                                                                                            tgtCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCaa                       <  3:354014/149‑1 (MQ=255)
                                                                                                                              tCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAAtt                     <  4:60620/149‑1 (MQ=255)
                                                                                                                                ggCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTg                    >  7:257752/1‑148 (MQ=255)
                                                                                                                                 gCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTACAGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGAc                  >  1:94534/1‑149 (MQ=255)
                                                                                                                                       gagagCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCt                                                <  6:299333/109‑1 (MQ=255)
                                                                                                                                       gagagCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCt                                                >  5:299333/5‑113 (MQ=255)
                                                                                                                                           gCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTg                                         >  2:300/1‑116 (MQ=255)
                                                                                                                                           gCGAGCGCCTGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTg                                         <  1:300/116‑1 (MQ=255)
                                                                                                                                                 gcATGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTTCAAGTGTCCAGTCACAGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCAAGGTTGGCGCTGTGGTTTGTCATAGGCTCCATGCAATTGACTCGGCGCTAATGCTTa  <  4:85482/149‑1 (MQ=255)
                                                                                                                                                 gcAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTa  <  4:90609/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGCGCCTGGCTGGCTTCAGGCAGCGCGTGGGTGCAGTGCGACAGGCGCTCGGAGCCTAGCAGTTCGGCCAGCAGCGAAGCGCCGGCAGTGTCCAGGCGGCCCAGCTGGCTGAGGTCGGCGACGGTGTCGGCAGGGCAATGCGAGCGCAGGCGCTCGCTCTCGCGCTTGAGGTTGGCGTAGTGTGCCAGGGTCCAGTCACCGGTAATACGCAGGCAGGCCGGTTGGCTGCTGGTGTCCAGGGTGGCGCTGGGGTTGGTCATAGGCTCCATGCAATTGACTCGGCGCTAAGGCTTA  >  NC_002947/150681‑150974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑