Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,155,453	G→T	8.0%	R204L (CGC→CTC) ‡	PP_1013 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,155,453	0	G	T	8.0%	241.6 / 8.6	75	R204L (CGC→CTC) ‡	PP_1013	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (37/32);  new base T (3/3);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCCGCGCTCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGC  >  NC_002947/1155310‑1155597                                                                                                                                                |                                                                                                                                                 gCCGCGCTCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGc                                                                                                                                             >  6:303397/1‑149 (MQ=255)  ccGCGCTCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGc                                                                                                                                             <  6:36173/148‑1 (MQ=255)        tCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGc                                                                                                                                             >  2:179655/1‑142 (MQ=255)        tCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGc                                                                                                                                             <  1:179655/142‑1 (MQ=255)                ccATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCg                                                                                                                              <  3:361390/149‑1 (MQ=255)                   ttattCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCt                                                                                                                           >  7:297711/1‑149 (MQ=255)                     attCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCtt                                                                                                                          <  8:297711/148‑1 (MQ=38)                      ttCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTc                                                                                                                         <  1:36521/148‑1 (MQ=38)                       tCGCTGACGATGGATCCGCTGATACCCCCGGAACTGGTCACTCTGATCCGCTTTGGCGAGGTCGAATGGTTGTACCTCGCCTCTCTGTTGCCCGAACCTTTCACGGGGCTGGTAGCCGAACGCCTGCCGCGCCAGCTGATCGGCttga                                                                                                                        <  6:373737/148‑3 (MQ=255)                         gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCg                                                                                                                     >  6:40697/1‑149 (MQ=255)                         gCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCg                                                                                                                     >  4:372858/1‑149 (MQ=255)                             aCCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCt                                                                                                                 >  6:314708/1‑149 (MQ=255)                                    aGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTGGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACg                                                                                                          <  1:261361/149‑1 (MQ=255)                                      ccGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGc                                                                                                        >  3:45979/1‑149 (MQ=255)                                        gCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCt                                                                                                      >  8:56662/1‑149 (MQ=255)                                        gCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCt                                                                                                      >  8:230817/1‑149 (MQ=255)                                          tGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGAt                                                                                                                                <  2:355335/121‑1 (MQ=255)                                          tGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGAt                                                                                                                                >  1:355335/1‑121 (MQ=255)                                               cccccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgtt                                                                                               >  3:224234/1‑149 (MQ=255)                                                ccccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgttg                                                                                              <  8:83752/149‑1 (MQ=255)                                                ccccGGTACTGGGCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCGGTTGCCCGAACCGTACACGGGGCGGGAAGCCGAACGCCTGCCGCGCCAGCAGAGCGGCTTCATCGTGCTGACCACGGCCttgctgttg                                                                                              <  3:42831/149‑1 (MQ=255)                                                 cccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgttgc                                                                                             >  8:231800/1‑149 (MQ=255)                                                  ccGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgttgct                                                                                            <  5:303397/149‑1 (MQ=255)                                                   cGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgttgctg                                                                                           <  7:230817/149‑1 (MQ=255)                                                         tGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATc                                                                                     >  8:267671/1‑149 (MQ=255)                                                           gTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCgg                                                                                   >  7:36529/1‑149 (MQ=255)                                                           gTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCgg                                                                                   >  3:170024/1‑149 (MQ=255)                                                           gTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCg                                                                                    <  5:40697/148‑1 (MQ=255)                                                            tCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGc                                                                                  <  3:216009/149‑1 (MQ=255)                                                                gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGcc                                                                                                       <  8:321360/124‑1 (MQ=255)                                                                gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGcc                                                                                                       >  7:321360/1‑124 (MQ=255)                                                                gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgttg                                                                                              <  6:12965/133‑1 (MQ=255)                                                                gCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttgctgttg                                                                                              >  5:12965/1‑133 (MQ=255)                                                                  aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGcc                                                                                                                                       >  3:124004/1‑90 (MQ=255)                                                                  aGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGcc                                                                                                                                       <  4:124004/90‑1 (MQ=255)                                                                        gCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTTCAGTTCATCGGCTTGCTGGTGCAc                                                                      <  7:56662/149‑1 (MQ=255)                                                                        gCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTg                                                                         <  7:191915/146‑1 (MQ=255)                                                                        ccTTGGGCGAGGGCGAATCGATGTACATCGACTCGCTGTTGCCAGAACCGTACACGCGGCTCGAAGCCTAAAGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGATGACAACGGCCTTGATGTTGATGTTCATCGCCTTGCTGGTg                                                                         >  8:191915/2‑146 (MQ=255)                                                                             ggcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCa                                                                 <  7:267671/149‑1 (MQ=255)                                                                             ggcgagggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCa                                                                 <  4:45979/149‑1 (MQ=255)                                                                               cgagggcgaATGCTTGTACATCGCCTCCCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCATGCCGCGCCAGCAGATCGGCTTCATCGAGCTGACCACGGCCTTGCTGTTGCTGTTCAACGGCTTGCTGGTGCACTGGCaaa                                                               >  3:108030/1‑149 (MQ=255)                                                                                   ggcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTg                                                           <  4:170024/149‑1 (MQ=255)                                                                                    gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttg                                                                                                    <  8:343320/107‑1 (MQ=255)                                                                                    gcgaATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCttg                                                                                                    >  7:343320/1‑107 (MQ=255)                                                                                               tACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTg                                                                               >  7:245149/1‑117 (MQ=255)                                                                                               tACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTg                                                                               <  8:245149/117‑1 (MQ=255)                                                                                               tACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAgc                                                <  7:231800/148‑1 (MQ=255)                                                                                                 cATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                      <  3:21601/76‑1 (MQ=255)                                                                                                 cATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATc                                                                                                                      >  4:21601/1‑76 (MQ=255)                                                                                                  aTCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCATGGCCTTGCTGTTGCTGTTCATCGGCTTGCTg                                                                            >  6:94387/1‑117 (MQ=255)                                                                                                  aTCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCATGGCCTTGCTGTTGCTGTTCATCGGCTTGCTg                                                                            <  5:94387/117‑1 (MQ=255)                                                                                                  aTCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCt                                            >  2:256132/1‑149 (MQ=255)                                                                                                  aTCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCt                                            >  4:317159/1‑149 (MQ=255)                                                                                                    cGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGGTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTgg                                          >  6:342223/1‑149 (MQ=255)                                                                                                    cGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                         >  7:12712/1‑134 (MQ=255)                                                                                                    cGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                         <  8:12712/134‑1 (MQ=255)                                                                                                                gCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTg                                           <  4:217976/136‑1 (MQ=255)                                                                                                                gCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTg                                           >  3:217976/1‑136 (MQ=255)                                                                                                                 cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCgg                                                                                   <  5:373540/95‑1 (MQ=255)                                                                                                                 cccGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCgg                                                                                   >  6:373540/1‑95 (MQ=255)                                                                                                                   cGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCgag                           <  3:317159/149‑1 (MQ=255)                                                                                                                    gAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCgaga                          <  5:314708/149‑1 (MQ=255)                                                                                                                     aaCCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGc                                                         >  4:166295/1‑117 (MQ=255)                                                                                                                             acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCt                 >  6:329156/1‑149 (MQ=255)                                                                                                                             acGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCt                 >  4:352904/1‑149 (MQ=255)                                                                                                                               ggggCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTgg               >  4:300806/1‑149 (MQ=255)                                                                                                                                  cgatgAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTg            >  3:367442/5‑149 (MQ=38)                                                                                                                                   cTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTAGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGc           <  8:36529/149‑1 (MQ=255)                                                                                                                                    tGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGcc          >  5:345677/1‑149 (MQ=255)                                                                                                                                    atgAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCt                 <  6:44469/140‑1 (MQ=38)                                                                                                                                    atgAAGCCGATCTGCTGGCGCGGCAGGCGTTCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCt                 >  5:44469/3‑142 (MQ=38)                                                                                                                                      gAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGCCGa        >  4:301402/1‑149 (MQ=255)                                                                                                                                      gAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGCCGa        <  4:224234/149‑1 (MQ=255)                                                                                                                                      gAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGCCGa        >  1:193765/1‑149 (MQ=255)                                                                                                                                            gAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGgc  >  4:363892/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                 GCCGCGCTCATGGGCCCATTATTCGCTGACCTTGGAGCCGCTGAAACCCCCGGTACTGGTCACGCAGATCCGCTTGGGCGAGGGCGAATGGTTGTACATCGCCTCGCTGTTGCCCGAACCGTACACGGGGCTGGAAGCCGAACGCCTGCCGCGCCAGCAGATCGGCTTCATCGTGCTGACCACGGCCTTGCTGTTGCTGTTCATCGGCTTGCTGGTGCACTGGCAAAGCTGGCCACTGAAGCGCCTGGCGCGGGCCGCCCGAGAGATGTCGCTGGGTGCCGATGTGGC  >  NC_002947/1155310‑1155597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑