Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,173,015 C→A 10.7% R13L (CGC→CTC)  xseA ← exodeoxyribonuclease 7 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,173,0150CA10.7% 154.5 / 7.3 55R13L (CGC→CTC) xseAexodeoxyribonuclease 7 large subunit
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base A (3/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGCCCTGCATC  >  NC_002947/1172877‑1173159
                                                                                                                                          |                                                                                                                                                
tCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGccc                                                                                                                                        <  2:272281/149‑1 (MQ=255)
tCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGc                                                                                                                                          <  3:193487/147‑1 (MQ=255)
  cTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGa                                                                                                                                      <  2:372433/149‑1 (MQ=255)
    tGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGt                                                                                                                                    >  2:209989/1‑149 (MQ=255)
        ggTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGtt                                                                                                                                <  2:340432/149‑1 (MQ=255)
              gTACATGTGGCCGGACGCCGGGCGGGGGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAg                                                                                                                          <  6:299934/149‑1 (MQ=255)
                  aTGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTc                                                                                                                      >  3:136994/1‑149 (MQ=255)
                  aTGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTc                                                                                                                      >  3:367756/1‑149 (MQ=255)
                  aTGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTc                                                                                                                      >  1:264632/1‑149 (MQ=255)
                   tgtgGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCt                                                                                                                     <  5:193241/149‑1 (MQ=255)
                       gCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTcc                                                                                                                                             <  2:141300/121‑1 (MQ=255)
                       gCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTcc                                                                                                                                             >  1:141300/1‑121 (MQ=255)
                         cGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGGTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGAt                                                                                                                >  5:95452/1‑148 (MQ=255)
                                  ggcgggcgAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCACGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCAtggctgg                                                                                                       >  4:220230/1‑148 (MQ=255)
                                       gcgAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGTCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGcatcat                                                                                                 <  7:58211/149‑1 (MQ=255)
                                        cgAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGcatcat                                                                                                 >  5:294910/1‑148 (MQ=255)
                                         gAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATaa                                                                                               <  4:243114/149‑1 (MQ=255)
                                             ttGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCAGGCGCGGCCGTTGAGTTGGCCGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGca                                                                                                     >  1:205459/1‑139 (MQ=255)
                                             ttGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCAGGCGCGGCCGTTGAGTTGGCCGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGca                                                                                                     <  2:205459/139‑1 (MQ=255)
                                               ggAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATaa                                                                                               >  8:190626/1‑143 (MQ=255)
                                               ggAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATaa                                                                                               <  7:190626/143‑1 (MQ=255)
                                                 agaTTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATc                                                                                       >  6:335988/1‑149 (MQ=255)
                                                   aTTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCttt                                                                                                                   >  3:127693/1‑119 (MQ=255)
                                                   aTTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCttt                                                                                                                   <  4:127693/119‑1 (MQ=255)
                                                    tttCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGa                                                                                                                 <  5:368502/120‑1 (MQ=255)
                                                    tttCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTATTTGa                                                                                                                 >  6:368502/1‑120 (MQ=255)
                                                         ccTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATc                                                                                                               >  3:361761/1‑117 (MQ=255)
                                                         ccTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATc                                                                                                               <  4:361761/117‑1 (MQ=255)
                                                                 ccacACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTtgtctgt                                                                       <  2:264632/149‑1 (MQ=255)
                                                                   acacGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTgtctgtct                                                                     >  4:312224/1‑149 (MQ=255)
                                                                           cGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGCCGGTAAGGACCACGCGGTCCCGCCCGAGTCTTTCGAAAGGGTATTTGATCATGGGTGGCATCATAAATGACATCGCCCCCGGTTGTATGTATTGCCCCTa                                                             >  6:364334/1‑149 (MQ=255)
                                                                                           gcagcaCGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGAcc                                              >  6:174796/1‑148 (MQ=255)
                                                                                                  gcgGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATATATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCtt                                      >  4:13051/1‑149 (MQ=255)
                                                                                                     ggCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATATATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTtgtg                                   <  3:13051/149‑1 (MQ=255)
                                                                                                      gcgcGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTgg                                  <  3:312224/149‑1 (MQ=255)
                                                                                                        gcgGCCGTTGAGTTGGCTGACTGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGga                                <  5:174796/149‑1 (MQ=255)
                                                                                                                      ggCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGt                   <  1:209989/148‑1 (MQ=255)
                                                                                                                      ggCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGt                   <  5:364334/148‑1 (MQ=255)
                                                                                                                      ggCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGt                   <  6:294910/148‑1 (MQ=255)
                                                                                                                           aCGGTAAGGACCCCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCTGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCtt              <  3:220230/148‑1 (MQ=255)
                                                                                                                               tAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAAc                       >  6:312025/1‑135 (MQ=255)
                                                                                                                               tAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAAc                       <  5:312025/135‑1 (MQ=255)
                                                                                                                               tAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGcc         >  4:104502/1‑149 (MQ=255)
                                                                                                                               tAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGcc         >  8:214042/1‑149 (MQ=255)
                                                                                                                               gctggACCGCGAGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCa                                                       >  2:208718/4‑103 (MQ=255)
                                                                                                                               gctggACCGCGAGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCa                                                       <  1:208718/100‑1 (MQ=255)
                                                                                                                               gctggACCGCGAGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAg           <  1:94835/144‑1 (MQ=255)
                                                                                                                               gctggACCGCGAGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAg           >  2:94835/4‑147 (MQ=255)
                                                                                                                                ctggACCGCGAGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTtg                                     <  7:9413/118‑1 (MQ=255)
                                                                                                                                ctggACCGCGAGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTtg                                     >  8:9413/3‑120 (MQ=255)
                                                                                                                                  ggACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTc             <  5:332673/142‑1 (MQ=255)
                                                                                                                                  ggACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTc             >  6:332673/1‑142 (MQ=255)
                                                                                                                                   gACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGCCCTg      >  8:37141/1‑148 (MQ=255)
                                                                                                                                    aCCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGCCCTGc     >  6:204454/1‑148 (MQ=255)
                                                                                                                                      cTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGCCCTGCAtc  >  3:64410/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TCCTTGAGGGTGAAGTACATGTGGCCGGACGCCGGGCGGGCGAGGTTGGAGATTTCGCCTTCCACCCACACGCTGCGGAAAACGTCTTCCAGCAGCACGCGGGCGCGGCCGTTGAGTTGGCTGACGGTAAGGACCTCGCGGTCCAGCCCGAGTCGTTCGAAAGGGTCTTTGATCATGGCTGGCATCATAAATGACATCGGCCCCGGTTGTCTGTCTTGCCCCTAGTGTCAGCCTTGACCCAATCCTTGTGGGAGACCGCAACCGGTGCCTTCAGCCCTGCATC  >  NC_002947/1172877‑1173159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑