Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,194,641 G→A 5.8% T260T (ACG→ACA)  uxpA → lipoprotein UxpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,194,6410GA 5.8% 213.1 / 2.0 69T260T (ACG→ACA) uxpAlipoprotein UxpA
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base A (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGCCCGTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCG  >  NC_002947/1194494‑1194789
                                                                                                                                                   |                                                                                                                                                    
tGCCCGTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACgg                                                                                                                                                     <  2:297703/149‑1 (MQ=255)
     gTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGcccc                                                                                                                                                <  4:132590/149‑1 (MQ=255)
          gCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCGTGGCCGATGCAGGCACGGGCCCCGGCCt                                                                                                                                           >  1:281142/1‑149 (MQ=255)
            aGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCtgt                                                                                                                                         <  2:6791/149‑1 (MQ=255)
            aGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCtgt                                                                                                                                         <  6:107856/149‑1 (MQ=255)
                    gCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGtggctggc                                                                                                                                  <  2:248668/148‑1 (MQ=255)
                    gCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTggctggct                                                                                                                                 >  7:224127/1‑149 (MQ=255)
                      gTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGa                                                                                                                               >  1:193534/1‑149 (MQ=255)
                            gaccCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGt                                                                                                                         <  2:189129/149‑1 (MQ=255)
                             accCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTg                                                                                                                        <  3:344183/149‑1 (MQ=255)
                              cccGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGc                                                                                                                       <  7:362204/149‑1 (MQ=255)
                               ccGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGcc                                                                                                                      >  3:160199/1‑149 (MQ=255)
                                cGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCg                                                                                                                     <  8:224127/149‑1 (MQ=255)
                                     gCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAAt                                                                                                                >  1:56771/1‑149 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGc                                                                                                                       <  6:220691/140‑1 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGc                                                                                                                       >  5:220691/1‑140 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATc                                                                                                               >  1:282659/1‑148 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACAGGCCGGGGCCCGtgcct                                                                                                                                     <  4:61636/126‑1 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACAGGCCGGGGCCCGtgcct                                                                                                                                     >  3:61636/1‑126 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGCCACAGGCCGGGGCCCGtgcct                                                                                                                                     >  5:77151/1‑126 (MQ=255)
                                       ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGCCACAGGCCGGGGCCCGtgcct                                                                                                                                     <  6:77151/126‑1 (MQ=255)
                                          ggATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGtggc                                                                                                                                      >  3:106712/1‑122 (MQ=255)
                                          ggATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGtggc                                                                                                                                      <  4:106712/122‑1 (MQ=255)
                                             tCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGc                                                                                                                                             <  8:79896/112‑1 (MQ=255)
                                             tCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGc                                                                                                        >  5:81481/1‑149 (MQ=255)
                                               aGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtg                                                                                                      <  2:56771/149‑1 (MQ=255)
                                                gAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgt                                                                                                     >  2:78267/1‑149 (MQ=255)
                                                gAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgt                                                                                                     >  4:155595/1‑149 (MQ=255)
                                                gAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgt                                                                                                     >  6:185468/1‑149 (MQ=255)
                                                      ctcactcaAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTgcg                                                                                               >  8:342340/1‑149 (MQ=255)
                                                          ctcaAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCg                                                                                           <  4:160199/149‑1 (MQ=255)
                                                             aaaCAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTgg                                                                                        >  4:206960/1‑149 (MQ=255)
                                                                cAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCa                                                                                     <  7:318720/149‑1 (MQ=255)
                                                                     gTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGAcc                                                                                >  6:279776/1‑149 (MQ=255)
                                                                     gTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCACGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGACCGTGGTCAGGAcc                                                                                >  7:302528/1‑149 (MQ=255)
                                                                        ccAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCt                                                                               <  4:160665/147‑1 (MQ=255)
                                                                        ccAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCt                                                                               >  3:160665/1‑147 (MQ=255)
                                                                         ctgTCTGTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGGGCTGTTGTGCGCCCGTGGTCAGGACCtgtg                                                                            <  7:141098/147‑1 (MQ=255)
                                                                         cAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCtgtg                                                                            <  8:14120/149‑1 (MQ=255)
                                                                              tctGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCg                                                                       >  3:286119/1‑149 (MQ=255)
                                                                                  ctgcCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCAc                                                                    <  7:170206/148‑1 (MQ=255)
                                                                                     ccGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGcctcgaccg                                                                  <  2:282659/147‑8 (MQ=255)
                                                                                        gTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCGTGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGa                                                             <  2:281142/149‑1 (MQ=255)
                                                                                           ttCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGa                                                                                  >  7:106538/1‑125 (MQ=255)
                                                                                           ttCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGa                                                                                  <  8:106538/125‑1 (MQ=255)
                                                                                           ttCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAgg                                                           <  7:342340/148‑1 (MQ=255)
                                                                                              gAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCAc                                                       >  3:93148/1‑149 (MQ=255)
                                                                                                  aaGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGATCGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAg                                                   <  2:193534/149‑1 (MQ=255)
                                                                                                    gCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGc                                                 >  1:149615/1‑149 (MQ=255)
                                                                                                      ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTc                                                                                      >  8:104345/1‑110 (MQ=255)
                                                                                                      ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTc                                                                                      <  7:104345/110‑1 (MQ=255)
                                                                                                      ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGc                                                 >  1:37585/1‑147 (MQ=255)
                                                                                                      ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCa                                               >  8:84329/1‑149 (MQ=255)
                                                                                                       cGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAg                                              >  5:154471/1‑149 (MQ=255)
                                                                                                         gCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCg                                            <  6:81481/149‑1 (MQ=255)
                                                                                                          cACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCgg                                           <  1:78267/149‑1 (MQ=255)
                                                                                                             gAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgttgt                                                                                                  >  4:286857/1‑91 (MQ=255)
                                                                                                             gAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgttgt                                                                                                  <  3:286857/91‑1 (MQ=255)
                                                                                                             gAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCg                                        >  4:241613/1‑149 (MQ=255)
                                                                                                                      cTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGcc                               >  6:106991/1‑149 (MQ=255)
                                                                                                                       tggtggtggCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGCCCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGAGTGCCg                              >  4:255567/1‑149 (MQ=255)
                                                                                                                         gtggtggCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCg                              <  5:279776/147‑1 (MQ=255)
                                                                                                                         gtggtggCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCg                              <  5:185468/147‑1 (MQ=255)
                                                                                                                               gCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGtt                      <  3:155595/149‑1 (MQ=255)
                                                                                                                                 cATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTg                    >  7:211364/1‑149 (MQ=255)
                                                                                                                                  aTGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGt                       >  6:250299/1‑145 (MQ=255)
                                                                                                                                  aTGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGt                       <  5:250299/145‑1 (MQ=255)
                                                                                                                                     gCCGACGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCgg                <  4:2724/149‑1 (MQ=255)
                                                                                                                                      ccGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGc               <  7:287024/149‑1 (MQ=255)
                                                                                                                                      ccGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGc               >  8:287024/1‑149 (MQ=255)
                                                                                                                                                   gggCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCg  >  5:113072/1‑149 (MQ=255)
                                                                                                                                                   gggCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCg  >  3:235969/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCCCGTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCG  >  NC_002947/1194494‑1194789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑