Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,194,646	C→G	5.6%	P262R (CCC→CGC) ‡	uxpA →	lipoprotein UxpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,194,646	0	C	G	5.6%	230.2 / 4.1	71	P262R (CCC→CGC) ‡	uxpA	lipoprotein UxpA
Reads supporting (aligned to +/- strand):  ref base C (34/33);  new base G (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCGGTGCC  >  NC_002947/1194499‑1194794                                                                                                                                                    |                                                                                                                                                     gTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGcccc                                                                                                                                                     <  4:132590/149‑1 (MQ=255)      gCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCGTGGCCGATGCAGGCACGGGCCCCGGCCt                                                                                                                                                >  1:281142/1‑149 (MQ=255)        aGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCtgt                                                                                                                                              <  2:6791/149‑1 (MQ=255)        aGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCtgt                                                                                                                                              <  6:107856/149‑1 (MQ=255)                gCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGtggctggc                                                                                                                                       <  2:248668/148‑1 (MQ=255)                gCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTggctggct                                                                                                                                      >  7:224127/1‑149 (MQ=255)                  gTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGa                                                                                                                                    >  1:193534/1‑149 (MQ=255)                        gaccCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGt                                                                                                                              <  2:189129/149‑1 (MQ=255)                         accCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTg                                                                                                                             <  3:344183/149‑1 (MQ=255)                          cccGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGc                                                                                                                            <  7:362204/149‑1 (MQ=255)                           ccGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGcc                                                                                                                           >  3:160199/1‑149 (MQ=255)                            cGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCg                                                                                                                          <  8:224127/149‑1 (MQ=255)                                 gCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAAt                                                                                                                     >  1:56771/1‑149 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGc                                                                                                                            >  5:220691/1‑140 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGc                                                                                                                            <  6:220691/140‑1 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATc                                                                                                                    >  1:282659/1‑148 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACAGGCCGGGGCCCGtgcct                                                                                                                                          <  4:61636/126‑1 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACAGGCCGGGGCCCGtgcct                                                                                                                                          >  3:61636/1‑126 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGCCACAGGCCGGGGCCCGtgcct                                                                                                                                          <  6:77151/126‑1 (MQ=255)                                   ccAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGCCACAGGCCGGGGCCCGtgcct                                                                                                                                          >  5:77151/1‑126 (MQ=255)                                      ggATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGtggc                                                                                                                                           >  3:106712/1‑122 (MQ=255)                                      ggATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGtggc                                                                                                                                           <  4:106712/122‑1 (MQ=255)                                         tCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGc                                                                                                                                                  <  8:79896/112‑1 (MQ=255)                                         tCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGc                                                                                                             >  5:81481/1‑149 (MQ=255)                                           aGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtg                                                                                                           <  2:56771/149‑1 (MQ=255)                                            gAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgt                                                                                                          >  6:185468/1‑149 (MQ=255)                                            gAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgt                                                                                                          >  2:78267/1‑149 (MQ=255)                                            gAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgt                                                                                                          >  4:155595/1‑149 (MQ=255)                                                  ctcactcaAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTgcg                                                                                                    >  8:342340/1‑149 (MQ=255)                                                      ctcaAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCg                                                                                                <  4:160199/149‑1 (MQ=255)                                                         aaaCAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTgg                                                                                             >  4:206960/1‑149 (MQ=255)                                                            cAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCa                                                                                          <  7:318720/149‑1 (MQ=255)                                                                 gTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGAcc                                                                                     >  6:279776/1‑149 (MQ=255)                                                                 gTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCACGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGACCGTGGTCAGGAcc                                                                                     >  7:302528/1‑149 (MQ=255)                                                                    ccAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCt                                                                                    >  3:160665/1‑147 (MQ=255)                                                                    ccAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCt                                                                                    <  4:160665/147‑1 (MQ=255)                                                                     ctgTCTGTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGGGCTGTTGTGCGCCCGTGGTCAGGACCtgtg                                                                                 <  7:141098/147‑1 (MQ=255)                                                                     cAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCtgtg                                                                                 <  8:14120/149‑1 (MQ=255)                                                                          tctGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCg                                                                            >  3:286119/1‑149 (MQ=255)                                                                              ctgcCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCAc                                                                         <  7:170206/148‑1 (MQ=255)                                                                                 ccGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGcctcgaccg                                                                       <  2:282659/147‑8 (MQ=255)                                                                                    gTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCGTGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGa                                                                  <  2:281142/149‑1 (MQ=255)                                                                                       ttCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGa                                                                                       >  7:106538/1‑125 (MQ=255)                                                                                       ttCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGa                                                                                       <  8:106538/125‑1 (MQ=255)                                                                                       ttCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAgg                                                                <  7:342340/148‑1 (MQ=255)                                                                                          gAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCAc                                                            >  3:93148/1‑149 (MQ=255)                                                                                              aaGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGATCGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAg                                                        <  2:193534/149‑1 (MQ=255)                                                                                                gCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGc                                                      >  1:149615/1‑149 (MQ=255)                                                                                                  ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTc                                                                                           <  7:104345/110‑1 (MQ=255)                                                                                                  ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTc                                                                                           >  8:104345/1‑110 (MQ=255)                                                                                                  ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGc                                                      >  1:37585/1‑147 (MQ=255)                                                                                                  ccGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCa                                                    >  8:84329/1‑149 (MQ=255)                                                                                                   cGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAg                                                   >  5:154471/1‑149 (MQ=255)                                                                                                     gCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCg                                                 <  6:81481/149‑1 (MQ=255)                                                                                                      cACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCgg                                                <  1:78267/149‑1 (MQ=255)                                                                                                         gAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgttgt                                                                                                       >  4:286857/1‑91 (MQ=255)                                                                                                         gAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCtgttgt                                                                                                       <  3:286857/91‑1 (MQ=255)                                                                                                         gAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCg                                             >  4:241613/1‑149 (MQ=255)                                                                                                                  cTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGcc                                    >  6:106991/1‑149 (MQ=255)                                                                                                                   tggtggtggCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGCCCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGAGTGCCg                                   >  4:255567/1‑149 (MQ=255)                                                                                                                     gtggtggCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCg                                   <  5:279776/147‑1 (MQ=255)                                                                                                                     gtggtggCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCg                                   <  5:185468/147‑1 (MQ=255)                                                                                                                           gCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGtt                           <  3:155595/149‑1 (MQ=255)                                                                                                                             cATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTg                         >  7:211364/1‑149 (MQ=255)                                                                                                                              aTGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGt                            >  6:250299/1‑145 (MQ=255)                                                                                                                              aTGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGt                            <  5:250299/145‑1 (MQ=255)                                                                                                                                 gCCGACGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCgg                     <  4:2724/149‑1 (MQ=255)                                                                                                                                  ccGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGc                    <  7:287024/149‑1 (MQ=255)                                                                                                                                  ccGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGc                    >  8:287024/1‑149 (MQ=255)                                                                                                                                               gggCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCg       >  3:235969/1‑149 (MQ=255)                                                                                                                                               gggCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCg       >  5:113072/1‑149 (MQ=255)                                                                                                                                                 gCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCGGt     <  1:301590/149‑1 (MQ=255)                                                                                                                                                   cccGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCAcc                                                           <  4:144892/93‑1 (MQ=255)                                                                                                                                                   cccGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCAcc                                                           >  3:144892/1‑93 (MQ=255)                                                                                                                                                    ccGGCCTGTGGCTGGCTGAACGGGTGCCGCGAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCGGTGcc  >  5:260517/1‑149 (MQ=255)                                                                                                                                                    ccGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCGGTGcc  >  2:125926/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTATTGCAGTGGTTGGCGTGACCGACCCGTACGCCCAGGATCAGAAAGCCTCACTCAAACAGTGGTACCAGTCTCTGCTGCCGGTATTCGAGCAAGCCCGGCACGAGGCTGACCTGGTGGTGGCCATGGCCGATGCAGGCACGGGCCCCGGCCTGTGGCTGGCTGAACGGGTGCCGCAAATCGACGTGCTGTTGTGCGCCCGTGGTCAGGACCTGTGGCCCGCACCGGTCGAGGCCACCCAGGCCAGCGGTCGGCGCGTGCCGGTGCTGTTTGCCGGCTGCCGGGGCAGCGGTGCC  >  NC_002947/1194499‑1194794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑