Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,195,095	G→C	5.1%	A412P (GCA→CCA)	uxpA →	lipoprotein UxpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,195,095	0	G	C	5.1%	262.9 / 3.5	79	A412P (GCA→CCA)	uxpA	lipoprotein UxpA
Reads supporting (aligned to +/- strand):  ref base G (35/40);  new base C (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGCTGCTGCTGGACAA  >  NC_002947/1194951‑1195243                                                                                                                                                 |                                                                                                                                                     gACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGc                                                                                                                                                  >  4:232592/1‑149 (MQ=255)   ccAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgg                                                                                                                                                >  6:21075/1‑149 (MQ=255)   ccAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgg                                                                                                                                                >  1:310237/1‑149 (MQ=255)     aGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgggg                                                                                                                                              >  1:50146/1‑149 (MQ=255)      gCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCggggg                                                                                                                                             <  3:236719/149‑1 (MQ=255)      gCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCggggg                                                                                                                                             >  5:66443/1‑149 (MQ=255)      gCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCggggg                                                                                                                                             <  2:310237/149‑1 (MQ=255)         gCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcgg                                                                                                                                          <  6:216774/149‑1 (MQ=255)          cTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcggc                                                                                                                                         <  5:21075/149‑1 (MQ=255)          cTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcggc                                                                                                                                         >  6:157798/1‑149 (MQ=255)          cTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggcggc                                                                                                                                         <  1:67892/149‑1 (MQ=255)             gCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGCCTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGcc                                                                                                                                        <  2:300201/147‑1 (MQ=255)               ccGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTa                                                                                                                                    <  6:283261/149‑1 (MQ=255)                cgcgcgCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTAc                                                                                                                                   <  6:66443/149‑1 (MQ=255)                       cccAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGaa                                                                                                                            >  5:108837/1‑149 (MQ=255)                         cAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggc                                                                                                                                            <  1:364638/131‑1 (MQ=255)                         cAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGggc                                                                                                                                            >  2:364638/1‑131 (MQ=255)                           ggCCGTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATc                                                                                                                        <  4:201941/149‑1 (MQ=255)                              cTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGa                                                                                                                     >  3:107947/1‑149 (MQ=255)                                 gtgGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGAt                                                                                                                    >  6:81717/1‑147 (MQ=255)                                       ccgcGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTa                                                                                                            <  6:319350/149‑1 (MQ=255)                                               cccGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAgg                                                                                                     <  2:50146/148‑1 (MQ=255)                                                ccGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGc                                                                                                    >  6:334755/1‑148 (MQ=255)                                                ccGAGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGc                                                                                                    >  2:225345/1‑148 (MQ=255)                                                 cGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTa                                                                                                  >  1:249412/1‑149 (MQ=255)                                                   tGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTAcc                                                                                                <  8:67989/149‑1 (MQ=255)                                                     gCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTAcccc                                                                                              >  1:141023/1‑149 (MQ=255)                                                         tAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCgcgc                                                                                          <  5:100723/149‑1 (MQ=255)                                                              gggACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTg                                                                                     >  4:175732/1‑149 (MQ=255)                                                              gggACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCACTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTg                                                                                     >  1:210213/1‑149 (MQ=255)                                                               ggACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTgg                                                                                    <  5:334755/149‑1 (MQ=255)                                                               ggACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTg                                                                                     <  2:249412/148‑1 (MQ=255)                                                                   tgcctgcTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTc                                                                                  >  8:8864/1‑147 (MQ=255)                                                                     cctgcTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGa                                                                                <  3:175732/147‑1 (MQ=255)                                                                     cctgcTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAgg                                                                              >  6:22227/1‑149 (MQ=255)                                                                     cctgcTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAg                                                                               <  6:85963/148‑1 (MQ=255)                                                                     cctgcTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAg                                                                               <  4:182931/148‑1 (MQ=255)                                                                               cAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAccggcccg                                                                    >  1:293489/1‑149 (MQ=255)                                                                               cAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAc                                                                           <  1:166468/142‑1 (MQ=255)                                                                               cAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAc                                                                           >  2:166468/1‑142 (MQ=255)                                                                                 aGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCAccggcccgg                                                                   >  1:102238/1‑148 (MQ=255)                                                                                    cTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAgg                                                               <  5:22227/149‑1 (MQ=255)                                                                                       ggCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGtt                                                             <  2:141023/148‑1 (MQ=255)                                                                                        gCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGa                                                           <  6:108837/149‑1 (MQ=255)                                                                                         ccGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAg                                                          <  4:107947/149‑1 (MQ=255)                                                                                              gaTAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGt                                                     <  3:277107/149‑1 (MQ=255)                                                                                              gaTAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGt                                                     >  4:308964/1‑149 (MQ=255)                                                                                                   cagcaTGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTg                                                 >  1:251763/1‑148 (MQ=255)                                                                                                   cagcaTGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTg                                                 >  1:257094/1‑148 (MQ=255)                                                                                                     gcaTGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGaa                                              <  5:81717/149‑1 (MQ=255)                                                                                                     gcaTGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGa                                               >  2:102087/1‑148 (MQ=255)                                                                                                         gCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAAcg                                          >  3:121459/1‑149 (MQ=255)                                                                                                            cGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgggg                                                                                                                                              >  4:157976/1‑46 (MQ=255)                                                                                                            cGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCgggg                                                                                                                                              <  3:157976/46‑1 (MQ=255)                                                                                                            cGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAAcgccg                                       <  2:257094/149‑1 (MQ=255)                                                                                                            cGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAAcgccg                                       <  2:251763/149‑1 (MQ=255)                                                                                                                  gctgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAg                                  >  6:15998/1‑148 (MQ=255)                                                                                                                  gctgcCGGGCTTGCGTTATGACTACCCACTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAg                                  <  2:210213/148‑1 (MQ=255)                                                                                                                   ctgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAgg                                                               >  8:322820/1‑118 (MQ=255)                                                                                                                   ctgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAgg                                                               <  7:322820/118‑1 (MQ=255)                                                                                                                   ctgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGc                                 <  5:157798/148‑1 (MQ=255)                                                                                                                    tgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAAc                                           <  5:112007/137‑1 (MQ=255)                                                                                                                    tgcCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAAc                                           >  6:112007/1‑137 (MQ=255)                                                                                                                      ccGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAg                                                                               >  2:18299/1‑99 (MQ=255)                                                                                                                      ccGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAg                                                                               <  1:18299/99‑1 (MQ=255)                                                                                                                      ccGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAAtt                             <  2:293489/149‑1 (MQ=255)                                                                                                                          gCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTc                         >  2:85702/1‑149 (MQ=255)                                                                                                                              gCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGt                      >  8:29871/1‑148 (MQ=255)                                                                                                                              gCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGGGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGt                      <  3:237925/148‑1 (MQ=255)                                                                                                                               cGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGa                    <  3:308964/149‑1 (MQ=255)                                                                                                                                gTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTg                                                    <  2:192040/116‑1 (MQ=255)                                                                                                                                     gACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCt                                                                                                   >  5:271796/1‑64 (MQ=255)                                                                                                                                     gACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCt                                                                                                   <  6:271796/64‑1 (MQ=255)                                                                                                                                      cgcccccGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCaa                               >  1:173534/5‑131 (MQ=255)                                                                                                                                      cgcccccGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCaa                               <  2:173534/127‑1 (MQ=255)                                                                                                                                        cccccGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCgctgct           >  8:22606/3‑149 (MQ=255)                                                                                                                                          cccGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCgctgctg          <  1:225345/148‑1 (MQ=255)                                                                                                                                           ccGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCgctgctgct        >  5:275804/1‑149 (MQ=255)                                                                                                                                            cGCTGCCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGctgctgctg       <  7:22606/149‑1 (MQ=255)                                                                                                                                                 gCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGCCCCGCTGCTGCTGGacaa  >  7:242438/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGGGCTTGCGTTATGACTACCCGCTGGCAGCGGGGGCGGCCATTACCCGCGAACATCTGATCAGCCTTACCGGAGGCTACCCCGCGCCAGTGGTCGAGGCACCGGCCCGGCAGGTTGAGCAAGTGCTGGAAAACGCCGCTGAGCAATTGTTCGGTGACCCGCTGCTGCTGGACAA  >  NC_002947/1194951‑1195243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑