| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,209,720 | G→C | 6.9% | W110S (TGG→TCG) | ytnA → | amino acid permease YtnA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,209,720 | 0 | G | C | 6.9% | 186.8 / 3.9 | 58 | W110S (TGG→TCG) | ytnA | amino acid permease YtnA |
| Reads supporting (aligned to +/- strand): ref base G (27/27); new base C (2/2); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01 | |||||||||||
TCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCGGTGAAGGCTTTTGGT > NC_002947/1209573‑1209862 | tcGGCGTCCTGTCCATTCTGGTTATCATGCGCTACCTTGTTGAAATGGCCGTTCACAAAACTGTCGCCGGCTCGTTCATCCTTTATGCCCATGATTACCTCGTCCCATTGGCGTGATTACTGACCGGCTGGAACTACTGGTTCCtgtgt < 2:337890/149‑2 (MQ=255)tcGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTgg < 6:255052/149‑1 (MQ=255) ggcggcCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGc < 2:168192/148‑1 (MQ=255) ggcggcCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCt < 7:253995/149‑1 (MQ=255) gcggcCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTg > 1:43112/1‑149 (MQ=255) gcggcCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTg > 2:370511/1‑149 (MQ=255) ccTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGt < 8:46682/147‑1 (MQ=255) ccTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGt > 7:46682/1‑147 (MQ=255) cTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGCCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGAc > 3:51375/1‑149 (MQ=255) tGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGAcc > 4:332828/1‑149 (MQ=255) gTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGc < 5:251808/146‑1 (MQ=255) gATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGaaa > 6:270166/1‑149 (MQ=255) tCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATc > 8:301473/1‑149 (MQ=255) aTGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCAc < 2:211727/149‑1 (MQ=255) tGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACg < 1:370511/149‑1 (MQ=255) cccTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTg > 8:248892/1‑149 (MQ=255) cccTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTg < 8:179478/149‑1 (MQ=255) ccGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGACGAAATCACGGCAGTGG‑CCATCTACATggg > 3:346029/1‑148 (MQ=255) cGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCa > 1:9495/1‑149 (MQ=255) tGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATc < 7:248892/149‑1 (MQ=255) cacaACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCt < 3:332828/148‑1 (MQ=255) caACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCAc < 3:246893/120‑1 (MQ=255) caACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCAc > 4:246893/1‑120 (MQ=255) ccTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCtgtg > 3:123452/1‑89 (MQ=255) ccTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCtgtg < 4:123452/89‑1 (MQ=255) cTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTcccc > 8:195723/1‑149 (MQ=255) tGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTg > 5:18252/1‑91 (MQ=255) tGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTg < 6:18252/91‑1 (MQ=255) gCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGt > 6:243664/1‑149 (MQ=255) ggtCAGCCGATATGGCCAGGATTAGCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCt < 6:265623/147‑1 (MQ=255) gTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCt < 2:43112/149‑1 (MQ=255) aTGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGGCCCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTggg < 1:279861/148‑1 (MQ=255) gCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCt > 6:88809/1‑149 (MQ=255) gCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTAATGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGTCGTTCCCGGCTGGATCTGGGccat > 6:278602/1‑147 (MQ=255) ccAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTgg > 6:130222/1‑149 (MQ=255) ccAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTgg < 5:140824/149‑1 (MQ=255) ttACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGgcgc < 5:88809/149‑1 (MQ=255) cTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATgg > 6:227484/1‑97 (MQ=255) cTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATgg < 5:227484/97‑1 (MQ=255) ccATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGATCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTCG‑CCATCTACATGCGAATCTGGTTCCACGACGTTCCGCGCTCGATCTGGGCCCTGGCGGCGCTGGGCAGCAt > 1:61943/1‑149 (MQ=255) gCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGgc > 6:290816/1‑138 (MQ=255) gCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGgc < 5:290816/138‑1 (MQ=255) cGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGcgcg < 7:195723/149‑1 (MQ=255) tGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCtg > 8:57266/1‑149 (MQ=255) tGACCGGCTGGAACTACTGGTTCCTGTCGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTgg < 5:18861/126‑1 (MQ=255) tGACCGGCTGGAACTACTGGTTCCTGTCGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTgg < 5:13326/126‑1 (MQ=255) tGACCGGCTGGAACTACTGGTTCCTGTCGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTgg > 6:18861/1‑126 (MQ=255) tGACCGGCTGGAACTACTGGTTCCTGTCGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTgg > 6:13326/1‑126 (MQ=255) ccGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCtggtg < 7:301473/149‑1 (MQ=255) gCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTgg < 7:280580/120‑1 (MQ=255) gCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTgg > 8:280580/1‑120 (MQ=255) gAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCtg > 4:150635/1‑139 (MQ=255) gAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCtg < 3:150635/139‑1 (MQ=255) gAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCGGTGa < 5:270166/149‑1 (MQ=255) aaCTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCTGTGaa < 4:346029/149‑1 (MQ=255) aaCTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCGGTGaa < 2:9495/149‑1 (MQ=255) tactGGTTCCTGTGGCTGGTGACGTGTGTTGCCGAAATCACGGCAGTGG‑CAATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCGGGTGGCGGGGAAGGc > 6:28179/1‑149 (MQ=255) tactGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCg < 7:125838/76‑1 (MQ=255) tactGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCg > 8:125838/1‑76 (MQ=255) tGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCGGTGAAGGCttt > 5:244825/1‑149 (MQ=255) gTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCACCGACGTTCCGCTCTGGATCTGTCCCCTGGCGGCGCTGGGCAGCATGCGCCCGGTCAACCTGGTGGCGGTGAAGGCTGTTg > 8:298415/1‑149 (MQ=255) ccTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCGGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCGGTGAAGGCTTTTGGt < 5:243664/148‑1 (MQ=255) | TCGGCGGCCTGGCCATTCTGGTGATCATGCGCGCCCTTGGTGAAATGGCCGTGCACAACCCTGTCGCCGGCTCGTTCAGCCGTTATGCCCAGGATTACCTCGGCCCATTGGCGGGCTTCCTGACCGGCTGGAACTACTGGTTCCTGTGGCTGGTGACCTGTGTTGCCGAAATCACGGCAGTGG‑CCATCTACATGGGCATCTGGTTCCCCGACGTTCCGCGCTGGATCTGGGCCCTGGCGGCGCTGGGCAGCATGGGCGCGGTCAACCTGGTGGCGGTGAAGGCTTTTGGT > NC_002947/1209573‑1209862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |