| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 170,839 | T→G | 16.1% | intergenic (+20/‑550) | PP_0159 → / → PP_16SA | family III CoA‑transferase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 170,839 | 0 | T | G | 16.1% | 67.2 / 8.1 | 37 | intergenic (+20/‑550) | PP_0159/PP_16SA | family III CoA‑transferase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (17/14); new base G (4/2); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
GCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGG > NC_002947/170699‑170986 | gCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTAAAGTGGGGATGGATGATGGCGAGGTAAAGCGTATGGGTGATGATGGGGTGATATAGATGAAAAGCGGGGAGGCCGTCAGGcg > 1:307343/1‑148 (MQ=255) tGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGc > 1:67039/1‑149 (MQ=255) tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTccc > 7:151923/1‑141 (MQ=255) tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTccc < 8:151923/141‑1 (MQ=255) tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGGCACGCCTCCCCCCGTTGt > 2:162438/1‑149 (MQ=255) caccacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAATAGCGGGGAGGACGTCAGGCCTGCCCGCTTTTTTTTTAAGGGCTa > 7:355816/1‑148 (MQ=255) agaaTACCGATGTGGTGGTGGGGGATGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGaaa < 2:220030/146‑1 (MQ=255) aCCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTGAGGGCTCCCCGCGTTGTTTTTGAGCGCGTTCAAATGTTATTGATATTa > 8:332352/1‑147 (MQ=255) aCCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCg > 1:209707/1‑110 (MQ=255) aCCGAAGGGGTGCGGGGGGAAGT‑‑GTGGGGGCTGGATGCTGGCGAGGTACAGCGTCGGCGTGATGCGGGGGGGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCg < 2:209707/110‑1 (MQ=255) cGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAgg > 6:85273/1‑149 (MQ=255) gggtgCGGGGGCATGG‑‑GTTGGGGCTGGATGCTGGCGCGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGAc < 7:290063/148‑1 (MQ=39) ggAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGcc < 1:123735/142‑1 (MQ=255) ggAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGcc > 2:123735/1‑142 (MQ=255) t‑‑gtgGGGGCTGTAGGGTGGCGCGGTACAGCGTCTGCGGGATGCTGGGGTGGTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATccc < 8:355816/149‑1 (MQ=38) t‑‑gtTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATccc > 6:134142/1‑149 (MQ=255) ttGGGGGTGCATGCTGGGGAGTAACAGCGTCTGGGTTATGCGGGGGGGCTCTAGATGAAACGGGGGCAGCCCGTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATcccc < 1:150653/148‑1 (MQ=255) tgggggCTTGATGCTGGCGAGGTACAGCTGCTGCGGGATGCTGGGGTGCTCTATATGAAAAGCGGGTAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATcccc < 2:307343/146‑1 (MQ=255) ggCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTAAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat > 4:116938/1‑148 (MQ=255) cTGGATGCTGGCGAGGTCCTGCGTCTGCGTTATGCTGGGGGGCTATAGATTAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATccc < 5:37720/141‑1 (MQ=39) cTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCAGGCCTCCCCGCGTTTTTTTTAAGCGCGATAAAAGGTAATTTTAATTAAAAGTTTACGCCCACTCGAATaccccaaacg > 6:37720/1‑142 (MQ=255) tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCACGCCTCCCCGCTTTGTTTTTAAGCGCTATCAAAGGTAATTTAAATTAAAAGTTGACGCCCTCTCGAATCCCCTTATAATGCGaccc > 4:143739/1‑148 (MQ=39) cGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATACCCTTATAATGCGCCCCAATTACAGCGACATCGGaa > 5:94487/1‑148 (MQ=255) aTGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaa < 3:116938/149‑1 (MQ=255) gCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaa > 7:180924/1‑148 (MQ=255) cTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAAc > 4:83469/1‑149 (MQ=255) gggcactAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCtt < 5:293450/144‑1 (MQ=255) tGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGACAAACTCCTTg < 5:134142/149‑1 (MQ=255) tAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCGCTCGACTCCCCGTATAATGCGCCCCACTTGCAGCGACATCGGAACGAAAAACTGCTTGAGAtt > 5:207019/1‑149 (MQ=25) ttaaaaGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa < 7:332352/146‑1 (MQ=255) aaGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGt > 4:91691/1‑149 (MQ=255) cGGGGAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGtt > 7:295580/1‑147 (MQ=255) cGGGGAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGtt < 8:295580/147‑1 (MQ=255) ggggAGGCCTGACGGCCTCCCCGCTTTGTTTTTAAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTACCGCGACATAGGAACGAAAAACTCCTTGAGATTAAATGAGTTAag > 2:185976/1‑149 (MQ=18) ggggAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAag < 3:83469/149‑1 (MQ=255) ccGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGtt > 4:348459/1‑147 (MQ=255) gTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAgg < 5:85273/149‑1 (MQ=255) | GCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGT‑‑GTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGG > NC_002947/170699‑170986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |