Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,350,502	T→A	6.6%	K11I (AAA→ATA) ‡	PP_1176 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,350,502	0	T	A	6.6%	228.7 / 3.9	75	K11I (AAA→ATA) ‡	PP_1176	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/34);  new base A (2/3);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTC  >  NC_002947/1350359‑1350648                                                                                                                                                 |                                                                                                                                                    ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCAttt                                                                                                                                                    >  5:235088/1‑145 (MQ=255) ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCAttt                                                                                                                                                    <  6:235088/145‑1 (MQ=255)   gTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGc                                                                                                                                              >  7:117538/1‑149 (MQ=255)        tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                         >  5:286766/1‑149 (MQ=255)        tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                         >  7:23813/1‑149 (MQ=255)          aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                         <  7:189318/147‑1 (MQ=255)          aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCttg                                                                                                                                          <  8:117538/146‑1 (MQ=255)            gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGAt                                                                                                                                     <  2:108762/149‑1 (MQ=255)            gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCAAATGGCAGCCtttttatt                                                                                                                                     <  5:111895/149‑4 (MQ=255)              gaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGc                                                                                                                                   >  7:109514/1‑149 (MQ=255)                 cGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCt                                                                                                                                  >  3:138090/1‑147 (MQ=255)                  gggATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAg                                                                                                                               >  8:335538/1‑149 (MQ=255)                         cGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                         <  7:228410/148‑1 (MQ=255)                           cTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaa                                                                                                                      <  6:286766/149‑1 (MQ=255)                              tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACATGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCACGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                     <  1:289546/148‑1 (MQ=255)                              tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                     <  8:109514/147‑1 (MQ=255)                              tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                     <  4:138090/147‑1 (MQ=255)                              tgtgAGCGTTCTTAAGCACCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTg                                                                                                                                                   >  2:305101/1‑117 (MQ=255)                              tgtgAGCGTTCTTAAGCACCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTg                                                                                                                                                   <  1:305101/117‑1 (MQ=255)                                  aGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATcc                                                                                                               >  6:334201/1‑149 (MQ=255)                                    cGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAAAAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATcc                                                                                                               >  7:234521/1‑147 (MQ=255)                                      ttCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCGTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCGTTGTGGATGGTCAGCCCGGTCAAGACATCCCAGc                                                                                                           >  3:284827/1‑149 (MQ=255)                                            aGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaa                                                                                                                      >  2:163308/1‑132 (MQ=255)                                            aGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaa                                                                                                                      <  1:163308/132‑1 (MQ=255)                                                  ggAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCAc                                                                                              <  5:334201/149‑1 (MQ=255)                                                        cGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCt                                                                                        >  7:64123/1‑149 (MQ=255)                                                            aTCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGAt                                                                                     <  8:64123/148‑1 (MQ=255)                                                             tCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATAc                                                                                   >  6:133690/1‑149 (MQ=255)                                                                cgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTc                                                                                 >  8:2364/1‑148 (MQ=255)                                                                cgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTc                                                                                 >  8:7964/1‑148 (MQ=255)                                                                 agatctcAAAAACAGGCAAGGTCGGCAGACGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGa                                                                               <  5:30226/143‑1 (MQ=255)                                                                      ccAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCgg                                                                                                                          >  2:4051/1‑102 (MQ=255)                                                                      ccAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCgg                                                                                                                          <  1:4051/102‑1 (MQ=255)                                                                       cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCacac                                                                         >  8:98397/1‑149 (MQ=255)                                                                       cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCacac                                                                         >  2:295349/1‑149 (MQ=255)                                                                        aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGa                                                                               <  5:24612/142‑1 (MQ=255)                                                                        aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGa                                                                               >  6:24612/1‑142 (MQ=255)                                                                        aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACAcg                                                                        >  7:189624/1‑149 (MQ=255)                                                                        aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACAcg                                                                        >  3:183914/1‑149 (MQ=255)                                                                        aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGACA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACCCGa                                                                               >  6:30226/1‑142 (MQ=255)                                                                         aaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACAcgc                                                                       <  8:189624/149‑1 (MQ=255)                                                                             cAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTTTCGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtct                                                                   <  6:299577/149‑1 (MQ=255)                                                                              aGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                  <  8:23813/149‑1 (MQ=255)                                                                                         gCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCACCTCAA‑CGCACCACCCGTCTGATACACGATc                                                                             >  8:235858/1‑127 (MQ=255)                                                                                             ccGTATGTAACGTCCTTTTCTTCATCTTCCA‑TGCAATAAAAATGCCGCCATTTGGCATCCTTTTTTTTTCTCACCCCGGTCAATCAATCCCAGCTCAACCGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTtcttct                                                    <  1:93826/149‑1 (MQ=255)                                                                                             ccGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                  <  2:5521/134‑1 (MQ=255)                                                                                             ccGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                  >  1:5521/1‑134 (MQ=255)                                                                                             ccGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTACTtctc                                                   >  7:323861/1‑149 (MQ=255)                                                                                              cGTAGGAAACGTCATTTTCTGCTTCTGCCA‑GTCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTcc                                                  <  8:323861/149‑1 (MQ=255)                                                                                               gTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                  <  2:31226/132‑1 (MQ=255)                                                                                               gTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                  >  1:31226/1‑132 (MQ=255)                                                                                                           cTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCaa                                                                <  8:136405/122‑1 (MQ=255)                                                                                                           cTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCaa                                                                >  7:136405/1‑122 (MQ=255)                                                                                                           cTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTcc                                      <  1:295349/148‑1 (MQ=255)                                                                                                           cTGTTCTGCTTCTGCCA‑GGCAAAAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCa                                     <  8:234521/149‑1 (MQ=255)                                                                                                            tGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCATACCGGTCAATCAATACCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAt                                    >  5:272601/1‑149 (MQ=255)                                                                                                            tGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAt                                    >  5:285171/1‑149 (MQ=255)                                                                                                             gTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAt                                    >  2:357984/1‑148 (MQ=255)                                                                                                                 tgcttctgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATg                                   <  2:116986/145‑1 (MQ=255)                                                                                                                 tgcttctgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATg                                   >  1:116986/1‑145 (MQ=255)                                                                                                                  gcttctgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTcc                                                  >  7:112604/1‑129 (MQ=255)                                                                                                                  gcttctgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTcc                                                  <  8:112604/129‑1 (MQ=255)                                                                                                                      ctgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGc                            <  7:335538/147‑1 (MQ=255)                                                                                                                       tgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTc                                                                                         <  5:231254/85‑1 (MQ=255)                                                                                                                       tgcCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTc                                                                                         >  6:231254/1‑85 (MQ=255)                                                                                                                                 aTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaag                                                              <  2:191731/103‑1 (MQ=255)                                                                                                                                 aTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaag                                                              >  1:191731/1‑103 (MQ=255)                                                                                                                                    acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaag                                                           >  3:118369/3‑103 (MQ=255)                                                                                                                                    acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaag                                                           <  4:118369/101‑1 (MQ=255)                                                                                                                                    aaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAAc              <  5:133690/148‑1 (MQ=255)                                                                                                                                     aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg            <  6:285171/149‑1 (MQ=255)                                                                                                                                       aGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt          <  7:98397/149‑1 (MQ=255)                                                                                                                                       aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                  <  3:368987/141‑1 (MQ=255)                                                                                                                                       aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                  >  4:368987/1‑141 (MQ=255)                                                                                                                                        ggCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt         <  4:183914/149‑1 (MQ=255)                                                                                                                                             ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAg    >  3:177722/1‑149 (MQ=255)                                                                                                                                               aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAGCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGGTCTTCTCCTTCTTCAAGTCACTGATCTCGCTCATACATGGGAACGGGTTGGTAGTc  >  6:371494/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCA‑GGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAA‑CGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTC  >  NC_002947/1350359‑1350648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑