Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,376,321 A→T 6.6% intergenic (+30/+19) kup → / ← PP_1201 potassium transport system protein Kup/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,376,3210AT 6.6% 239.9 / 5.1 85intergenic (+30/+19)kup/PP_1201potassium transport system protein Kup/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/40);  new base T (4/2);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTG  >  NC_002947/1376177‑1376467
                                                                                                                                                |                                                                                                                                                  
gcgcTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGc                                                                                                                                                <  3:191340/149‑1 (MQ=255)
  gcTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCgg                                                                                                                                              >  1:278680/1‑149 (MQ=255)
     ggCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGccc                                                                                                                                           >  2:128709/1‑149 (MQ=255)
     ggCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGccc                                                                                                                                           <  7:122704/149‑1 (MQ=255)
      gCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGccc                                                                                                                                           <  7:122703/148‑1 (MQ=255)
        ggggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTg                                                                                                                                         <  6:123450/148‑1 (MQ=255)
        ggggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTg                                                                                                                                         >  5:123450/1‑148 (MQ=255)
        ggggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                        <  3:1582/149‑1 (MQ=255)
             aaCCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCtt                                                                                                                                   >  8:341035/1‑149 (MQ=255)
             aaCCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCtt                                                                                                                                   >  2:110609/1‑149 (MQ=255)
               ccTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCATAGCGGCGCGGAGCCTTg                                                                                                                                  >  3:40732/1‑148 (MQ=255)
                  gTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAg                                                                                                                              >  6:45224/1‑149 (MQ=255)
                  gTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAg                                                                                                                              >  1:356613/1‑149 (MQ=255)
                   ttCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCAAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGt                                                                                                                             >  5:141928/1‑149 (MQ=255)
                     cgcgTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGtt                                                                                                                            <  1:110609/148‑1 (MQ=255)
                     cgcgTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGtt                                                                                                                            <  2:356613/148‑1 (MQ=255)
                      gcgTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCt                                                                                                                          <  4:303520/149‑1 (MQ=255)
                        gTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGc                                                                                                                        >  3:326882/1‑149 (MQ=255)
                         ttCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCt                                                                                                                       <  2:278680/149‑1 (MQ=255)
                              gCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCAGAGCCGTGTAAGTTCTGCTCAGc                                                                                                                   >  2:314313/1‑148 (MQ=255)
                                    gaaTGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTg                                                                                                            <  6:348237/149‑1 (MQ=255)
                                       tGCCAATGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGAc                                                                                                         >  4:138725/1‑149 (MQ=255)
                                         ccAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACtt                                                                                                       >  1:369607/1‑149 (MQ=255)
                                          cAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTGAGTTCTGCTCAGCTACGCTGGACTTg                                                                                                      >  4:174754/1‑149 (MQ=255)
                                             cGGCAACTTGCGCTTCTTAAAACTGCCGTTCAACCGGCTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATAAAGCATCCTCAGGGCCGCACAGCGGCCCTAAGCCTTGTAAGTTCTCATACACTACGCTGGACTTGACt                                                                                                   >  6:83674/1‑149 (MQ=255)
                                                       cgcTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGttt                                                                                         <  4:326882/149‑1 (MQ=255)
                                                        gcTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                        <  5:45224/149‑1 (MQ=255)
                                                             ttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtct                                                                                   >  1:164435/1‑149 (MQ=255)
                                                              tcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGcc                                                                                                                                     >  7:24710/1‑98 (MQ=255)
                                                              tcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGcc                                                                                                                                     <  8:24710/98‑1 (MQ=255)
                                                                aaCCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCg                                                                                 <  3:138725/148‑1 (MQ=255)
                                                                aaCCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGc                                                                                <  3:130762/149‑1 (MQ=255)
                                                                aaCCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGc                                                                                <  6:274297/149‑1 (MQ=255)
                                                                  ccTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCa                                                                              <  5:179201/149‑1 (MQ=255)
                                                                   atctcGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATCCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCaa                                                                             <  3:53758/145‑1 (MQ=255)
                                                                    tGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTAGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATATCGCCaa                                                                             >  4:99640/1‑148 (MQ=255)
                                                                      ccGTTGAAGCTGGTGATCGAGCTAGGGACCCATGGCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATc                                                                           <  4:40732/148‑1 (MQ=255)
                                                                      ccGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATc                                                                           <  2:369607/148‑1 (MQ=255)
                                                                      ccGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATc                                                                           <  6:301703/148‑1 (MQ=255)
                                                                        gTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCGGAGCCGTGTGAGTTCTGCTCAGCGACGCTGGACTTGCCTTGGCGCGGTTCGAGCTGGCGAAtagat                                                                        >  4:53758/1‑145 (MQ=255)
                                                                        gTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAACAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                  <  4:188716/139‑1 (MQ=255)
                                                                        gTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAACAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                  >  3:188716/1‑139 (MQ=255)
                                                                            aaCCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGc                                                                     <  5:287162/148‑1 (MQ=255)
                                                                              ccGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTg                                                                  >  3:323315/1‑149 (MQ=255)
                                                                              ccGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCGTGTGAGGTGTGCTCAGCTACGCTGGGCTTGGCTTGGGGCGGTTCGATCTCGCCAATCAACCGCTTg                                                                  >  6:244640/1‑149 (MQ=255)
                                                                              ccGGGTGATCGAGCTAGGGACCCAGCTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTTAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGc                                                                                <  3:15331/135‑1 (MQ=255)
                                                                               cGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCAATCTCGCCAATCAACCGCTTgg                                                                 <  6:212052/149‑1 (MQ=255)
                                                                                gggTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGc                                                                <  1:314313/149‑1 (MQ=255)
                                                                                          gCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGc                                                                                >  6:224383/1‑123 (MQ=255)
                                                                                          gCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGc                                                                                <  5:224383/123‑1 (MQ=255)
                                                                                          gCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCggg                                                      >  6:5209/1‑149 (MQ=255)
                                                                                              gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGttt                                                                                         <  6:69711/110‑1 (MQ=255)
                                                                                              gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGttt                                                                                         >  5:69711/1‑110 (MQ=255)
                                                                                              gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTa                                                    <  1:149170/147‑1 (MQ=255)
                                                                                              gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTa                                                    <  4:323315/147‑1 (MQ=255)
                                                                                               ggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGt                                                  >  7:293105/1‑148 (MQ=255)
                                                                                                   ccAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCAGAGCCTTGTCAGTTGTGCTAACCAACGCTGGACATTCCTTGGCGCGTTTCGATCTAGCCAATACACCCCTTGGCGACTGCCGGGGAGTTCtcg                                             >  8:141862/1‑149 (MQ=255)
                                                                                                    cAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCtcgt                                            >  6:169279/1‑149 (MQ=255)
                                                                                                     aGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCACAGCGGCCCGGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTACATCTCGCCAATAAACCGCTATGCGAGTGCCGGGTATTTCtcgtg                                           >  6:228223/1‑148 (MQ=255)
                                                                                                        tCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGaa                                        <  1:128709/149‑1 (MQ=255)
                                                                                                        tCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGaa                                        <  2:164435/149‑1 (MQ=255)
                                                                                                         cGAGATCTGATTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCgg                                                       <  1:370007/133‑1 (MQ=255)
                                                                                                         cGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTg                                                           >  8:59746/1‑129 (MQ=255)
                                                                                                         cGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTg                                                           <  7:59746/129‑1 (MQ=255)
                                                                                                          gagaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAgt                                      >  5:214307/1‑149 (MQ=255)
                                                                                                          gagaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAgt                                      >  7:282737/1‑149 (MQ=255)
                                                                                                           agaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCtcgt                                            <  6:166239/142‑1 (MQ=255)
                                                                                                           agaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCtcgt                                            >  5:166239/1‑142 (MQ=255)
                                                                                                                tGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGcc                                >  5:169969/1‑149 (MQ=255)
                                                                                                                  aaTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAgtggt                                   <  5:244640/144‑1 (MQ=255)
                                                                                                                  aaTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGc                                 <  7:141862/146‑1 (MQ=255)
                                                                                                                  aaTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCg                              >  1:81684/1‑149 (MQ=255)
                                                                                                                   aTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGa                                                              <  2:343119/116‑1 (MQ=255)
                                                                                                                   aTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGa                                                              >  1:343119/1‑116 (MQ=255)
                                                                                                                       ggTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAgg                         >  1:276308/1‑149 (MQ=255)
                                                                                                                           ataCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGt                     >  2:221992/1‑149 (MQ=255)
                                                                                                                           ataCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGt                     >  7:265696/1‑149 (MQ=255)
                                                                                                                                  agGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGATGTTGTGCCCGCCAGGCAGTTTCAGgc              <  6:141928/149‑1 (MQ=255)
                                                                                                                                     cTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCt           <  5:5209/149‑1 (MQ=255)
                                                                                                                                      tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTc                  >  7:28579/1‑141 (MQ=255)
                                                                                                                                      tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTc                  <  8:28579/141‑1 (MQ=255)
                                                                                                                                      tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCt           >  8:172537/1‑148 (MQ=255)
                                                                                                                                        aGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCa         <  2:81684/148‑1 (MQ=255)
                                                                                                                                         gggCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCAcc       >  8:169007/1‑149 (MQ=255)
                                                                                                                                              gCAGAGCGGCCCTGAGCCTTGTCAGTTCTGATCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTg  >  3:221863/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTG  >  NC_002947/1376177‑1376467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑