Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,388,037	G→T	8.6%	G151G (GGC→GGA)	PP_1209 ←	cold‑shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,388,037	0	G	T	8.6%	238.5 / 9.0	78	G151G (GGC→GGA)	PP_1209	cold‑shock protein
Reads supporting (aligned to +/- strand):  ref base G (35/36);  new base T (3/4);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

TTGGTCAACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACG  >  NC_002947/1387892‑1388184                                                                                                                                                      |                                                                                                                                                     ttGGTCAACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGccc                                                                                                                                                   <  5:69275/149‑1 (MQ=255)    gTCAACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAg                                                                                                                                                >  1:59055/1‑149 (MQ=255)     tCAACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGt                                                                                                                                               >  7:307619/1‑149 (MQ=255)       aaCGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGtct                                                                                                                                             >  5:214935/1‑149 (MQ=255)         cggcggTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCg                                                                                                                                           >  5:369731/1‑149 (MQ=255)          ggcggTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCgg                                                                                                                                          <  3:252556/149‑1 (MQ=255)              gTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGgaga                                                                                                                                      <  2:59055/149‑1 (MQ=255)               tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGAt                                                                                                                                     >  1:349417/1‑149 (MQ=255)               tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGAt                                                                                                                                     <  5:328152/149‑1 (MQ=255)               gttACTTCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGCCTAACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCggg                                                                                                                                         <  1:117294/148‑1 (MQ=255)                 tACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGATg                                                                                                                                    <  2:349417/148‑1 (MQ=255)                 tACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATg                                                                                                                                    <  1:151845/148‑1 (MQ=255)                   cTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGATGaca                                                                                                                                 <  8:183074/149‑3 (MQ=255)                     gCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAAc                                                                                                                                >  4:36745/1‑148 (MQ=255)                     gCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAAc                                                                                                                                >  3:133124/1‑148 (MQ=255)                      cAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTACGGAGATGAAAcc                                                                                                                               >  6:189763/1‑148 (MQ=255)                             gTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCtt                                                                                                                       >  8:244832/1‑149 (MQ=255)                              tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCT‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                      <  7:354106/149‑1 (MQ=255)                                ttCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCg                                                                                                                                           <  7:161087/126‑1 (MQ=255)                                ttCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCg                                                                                                                                           >  8:161087/1‑126 (MQ=255)                                    gCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                      >  3:333442/1‑143 (MQ=255)                                    gCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                      <  4:333442/143‑1 (MQ=255)                                     ccTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtg                                                                                                               <  8:307619/149‑1 (MQ=255)                                      cTGCAGGCCCTTGTCTCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGTCCCTCACCGCGTATGGCCCTATAGTTCACGAAGATGTCATCTCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                              <  5:189763/149‑1 (MQ=255)                                      cTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                              >  4:96970/1‑149 (MQ=255)                                       tGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGtt                                                                                                             >  2:215315/1‑149 (MQ=255)                                        gCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGtt                                                                                                             >  8:69707/1‑148 (MQ=255)                                            gCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAAc                                                                                                         >  7:267449/1‑148 (MQ=255)                                            gCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAAc                                                                                                         >  6:40997/1‑148 (MQ=255)                                                   tCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGa                                                                                                  <  2:246585/148‑1 (MQ=255)                                                   tCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGAc                                                                                                 <  7:244832/149‑1 (MQ=255)                                                    cgcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                              <  4:143357/135‑1 (MQ=255)                                                    cgcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                              >  3:143357/1‑135 (MQ=255)                                                    cgcgGTGCATCACCGAAAACTCCACGCGCTGCCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTAACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCACGAGTCTCGGGAGATGAAACCAAAGCCATTCGACGTGTTGAACCATTTGACg                                                                                                >  5:377792/1‑149 (MQ=255)                                                     gcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                      <  4:40106/126‑1 (MQ=255)                                                     gcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                      >  3:40106/1‑126 (MQ=255)                                                       ggTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑g                                                                                            >  7:227186/1‑149 (MQ=255)                                                           cATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCg                                                                                         <  8:227186/148‑1 (MQ=255)                                                               aCCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTAt                                                                                     <  4:175033/148‑1 (MQ=255)                                                               aCCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTAt                                                                                     <  6:369731/148‑1 (MQ=255)                                                                ccGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTc                                                                                                                     >  7:123488/1‑116 (MQ=255)                                                                ccGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTc                                                                                                                     <  8:123488/116‑1 (MQ=255)                                                                       cTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGc                                                                            >  4:109128/1‑149 (MQ=255)                                                                        tCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGcc                                                                           >  3:156472/1‑149 (MQ=255)                                                                          cACGCTCGGGGCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTTACCGCGGATGGACCGAAAGTGCACGAAGATGTGATCGCCCGAGTCTCGGGAGATGAAACAAAAGCCTTTCGACGTGATGAACCATTTGACGGT‑GCCGGTATCACGGTTGcc                                                                           <  6:377792/147‑1 (MQ=255)                                                                           aCGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGc                                                                        <  6:214935/149‑1 (MQ=255)                                                                             gcgcTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCGTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCAt                                                                      <  3:109128/149‑1 (MQ=255)                                                                                cTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCtt                                                                   <  4:133124/149‑1 (MQ=255)                                                                                cTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCtt                                                                   <  4:156472/149‑1 (MQ=255)                                                                                   gacTTAG‑‑‑‑ACCTGAATGCGGTGGCTCTCTGCGCGGATGGCCCGATTGTGCACGAAGATGTGATCGCCGGAGTCTCGGGAGTTGTTACGAAATCCTTTCGTCGTGTTTAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTtgt                                                                 <  4:238667/146‑1 (MQ=255)                                                                                   gCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGGCCGGTATCACGGTTGCCGGCATCTtgt                                                                 <  1:215315/149‑1 (MQ=255)                                                                                    ccTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGc                                                               <  8:267449/149‑1 (MQ=255)                                                                                              aCCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACgtg                                                         >  5:126963/1‑149 (MQ=255)                                                                                                cAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGtt                                                       >  2:182716/1‑149 (MQ=255)                                                                                                   aaTGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAg                                                    >  3:65409/1‑149 (MQ=255)                                                                                                       cGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCAGTTGACGGT‑GCCGGTATCACGGTTGCCCGCATCATGTGCCACCTGTTGAGTGcc                                                >  6:382614/1‑149 (MQ=255)                                                                                                               cTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCt                                        >  6:82073/1‑149 (MQ=255)                                                                                                               cTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACC‑AAGCCTTTCGACGTGtt                                                                                                             <  7:363059/80‑1 (MQ=255)                                                                                                               cTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACC‑AAGCCTTTCGACGTGtt                                                                                                             >  8:363059/1‑80 (MQ=255)                                                                                                                      gtgagggCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGt                                 <  5:132013/144‑1 (MQ=255)                                                                                                                           ggCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGt                                                   <  3:8256/126‑1 (MQ=255)                                                                                                                           ggCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGt                                                   >  4:8256/1‑126 (MQ=255)                                                                                                                            gCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGtgctgc                            <  3:96970/148‑1 (MQ=255)                                                                                                                              ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc                                     <  8:108082/137‑1 (MQ=255)                                                                                                                              ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCggg                         >  7:15729/1‑149 (MQ=255)                                                                                                                              ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCggg                         <  7:69707/149‑1 (MQ=255)                                                                                                                              ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCggg                         >  2:103302/1‑149 (MQ=255)                                                                                                                                    gTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGc                   >  2:49208/1‑149 (MQ=255)                                                                                                                                         cGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACg              <  4:65409/149‑1 (MQ=255)                                                                                                                                          tttggtttCATCTACCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACgg             >  6:102789/8‑149 (MQ=255)                                                                                                                                           aaGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGc            <  6:126963/149‑1 (MQ=255)                                                                                                                                                   aTCTCCCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCAGCATCTTGTGc                                                               >  6:99732/1‑90 (MQ=255)                                                                                                                                                   aTCTCCCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCAGCATCTTGTGc                                                               <  5:99732/90‑1 (MQ=255)                                                                                                                                                   aTCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGca    >  4:369621/1‑149 (MQ=255)                                                                                                                                                    tCGCCCGAGTCTCGGGTGATGAAACCAAAGCCTTTCGTCGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCAGGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcac   <  5:382614/149‑1 (MQ=255)                                                                                                                                                    tCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCGTTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcac   >  6:19069/1‑149 (MQ=255)                                                                                                                                                     cGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGccc                 >  3:325649/1‑134 (MQ=255)                                                                                                                                                     cGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGccc                 <  4:325649/134‑1 (MQ=255)                                                                                                                                                      gCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacg  <  1:103302/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     TTGGTCAACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCG‑‑‑‑ACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACG  >  NC_002947/1387892‑1388184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑