Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,388,051	A→C	7.9%	S147A (TCC→GCC)	PP_1209 ←	cold‑shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,388,051	0	A	C	7.9%	225.0 / 9.5	76	S147A (TCC→GCC)	PP_1209	cold‑shock protein
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base C (3/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCC  >  NC_002947/1387905‑1388199                                                                                                                                                   |                                                                                                                                                      gTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGgaga                                                                                                                                                     <  2:59055/149‑1 (MQ=255)  tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGAt                                                                                                                                                    >  1:349417/1‑149 (MQ=255)  tttACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGAt                                                                                                                                                    <  5:328152/149‑1 (MQ=255)    tACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGATg                                                                                                                                                   <  2:349417/148‑1 (MQ=255)    tACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATg                                                                                                                                                   <  1:151845/148‑1 (MQ=255)      cTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGATGaca                                                                                                                                                <  8:183074/149‑3 (MQ=255)        gCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAAc                                                                                                                                               >  4:36745/1‑148 (MQ=255)        gCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAAc                                                                                                                                               >  3:133124/1‑148 (MQ=255)         cAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTACGGAGATGAAAcc                                                                                                                                              >  6:189763/1‑148 (MQ=255)                gTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCtt                                                                                                                                      >  8:244832/1‑149 (MQ=255)                 tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCTACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                                     <  7:354106/149‑1 (MQ=255)                       gCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                                     <  4:333442/143‑1 (MQ=255)                       gCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                                     >  3:333442/1‑143 (MQ=255)                        ccTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtg                                                                                                                              <  8:307619/149‑1 (MQ=255)                         cTGCAGGCCCTTGTCTCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGTCCCTCACCGCGTATGGCCCTATAGTTCACGAAGATGTCATCTCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                                             <  5:189763/149‑1 (MQ=255)                         cTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                                             >  4:96970/1‑149 (MQ=255)                          tGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGtt                                                                                                                            >  2:215315/1‑149 (MQ=255)                           gCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGtt                                                                                                                            >  8:69707/1‑148 (MQ=255)                               gCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAAc                                                                                                                        >  7:267449/1‑148 (MQ=255)                               gCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAAc                                                                                                                        >  6:40997/1‑148 (MQ=255)                                      tCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGa                                                                                                                 <  2:246585/148‑1 (MQ=255)                                      tCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGAc                                                                                                                <  7:244832/149‑1 (MQ=255)                                       cgcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                                             >  3:143357/1‑135 (MQ=255)                                       cgcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACgtgt                                                                                                                             <  4:143357/135‑1 (MQ=255)                                       cgcgGTGCATCACCGAAAACTCCACGCGCTGCCCTTCGACCAGAATGCGGTGGCCCTAACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCACGAGTCTCGGGAGATGAAACCAAAGCCATTCGACGTGTTGAACCATTTGACg                                                                                                               >  5:377792/1‑149 (MQ=255)                                        gcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                                     >  3:40106/1‑126 (MQ=255)                                        gcgGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt                                                                                                                                     <  4:40106/126‑1 (MQ=255)                                          ggTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑g                                                                                                           >  7:227186/1‑149 (MQ=255)                                              cATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCg                                                                                                        <  8:227186/148‑1 (MQ=255)                                                  aCCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTAt                                                                                                    <  6:369731/148‑1 (MQ=255)                                                  aCCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTAt                                                                                                    <  4:175033/148‑1 (MQ=255)                                                   ccGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTc                                                                                                                                    >  7:123488/1‑116 (MQ=255)                                                   ccGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTc                                                                                                                                    <  8:123488/116‑1 (MQ=255)                                                          cTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGc                                                                                           >  4:109128/1‑149 (MQ=255)                                                           tCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGcc                                                                                          >  3:156472/1‑149 (MQ=255)                                                             cACGCTCGGGGCTTCGACCAGAATGCGGTGGCCCTTACCGCGGATGGACCGAAAGTGCACGAAGATGTGATCGCCCGAGTCTCGGGAGATGAAACAAAAGCCTTTCGACGTGATGAACCATTTGACGGT‑GCCGGTATCACGGTTGcc                                                                                          <  6:377792/147‑1 (MQ=255)                                                              aCGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGc                                                                                       <  6:214935/149‑1 (MQ=255)                                                                gcgcTGGCCTTCGACCAGAATGCGGTGGCCGTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCAt                                                                                     <  3:109128/149‑1 (MQ=255)                                                                   cTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCtt                                                                                  <  4:156472/149‑1 (MQ=255)                                                                   cTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCtt                                                                                  <  4:133124/149‑1 (MQ=255)                                                                      gacTTAGACCTGAATGCGGTGGCTCTCTGCGCGGATGGCCCGATTGTGCACGAAGATGTGATCGCCGGAGTCTCGGGAGTTGTTACGAAATCCTTTCGTCGTGTTTAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTtgt                                                                                <  4:238667/146‑1 (MQ=255)                                                                      gCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGGCCGGTATCACGGTTGCCGGCATCTtgt                                                                                <  1:215315/149‑1 (MQ=255)                                                                       ccTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGc                                                                              <  8:267449/149‑1 (MQ=255)                                                                             aCCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACgtg                                                                        >  5:126963/1‑149 (MQ=255)                                                                               cAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGtt                                                                      >  2:182716/1‑149 (MQ=255)                                                                                  aaTGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAg                                                                   >  3:65409/1‑149 (MQ=255)                                                                                      cGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCAGTTGACGGT‑GCCGGTATCACGGTTGCCCGCATCATGTGCCACCTGTTGAGTGcc                                                               >  6:382614/1‑149 (MQ=255)                                                                                              cTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCt                                                       >  6:82073/1‑149 (MQ=255)                                                                                              cTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACC‑AAGCCTTTCGACGTGtt                                                                                                                            <  7:363059/80‑1 (MQ=255)                                                                                              cTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACC‑AAGCCTTTCGACGTGtt                                                                                                                            >  8:363059/1‑80 (MQ=255)                                                                                                     gtgagggCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGt                                                <  5:132013/144‑1 (MQ=255)                                                                                                          ggCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGt                                                                  <  3:8256/126‑1 (MQ=255)                                                                                                          ggCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGt                                                                  >  4:8256/1‑126 (MQ=255)                                                                                                           gCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGtgctgc                                           <  3:96970/148‑1 (MQ=255)                                                                                                             ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc                                                    <  8:108082/137‑1 (MQ=255)                                                                                                             ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCggg                                        <  7:69707/149‑1 (MQ=255)                                                                                                             ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCggg                                        >  2:103302/1‑149 (MQ=255)                                                                                                             ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCggg                                        >  7:15729/1‑149 (MQ=255)                                                                                                                   gTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGc                                  >  2:49208/1‑149 (MQ=255)                                                                                                                        cGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACg                             <  4:65409/149‑1 (MQ=255)                                                                                                                         tttggtttCATCTACCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACgg                            >  6:102789/8‑149 (MQ=255)                                                                                                                          aaGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGc                           <  6:126963/149‑1 (MQ=255)                                                                                                                                  aTCTCCCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCAGCATCTTGTGc                                                                              <  5:99732/90‑1 (MQ=255)                                                                                                                                  aTCTCCCGAGACTCGGGCGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCAGCATCTTGTGc                                                                              >  6:99732/1‑90 (MQ=255)                                                                                                                                  aTCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGca                   >  4:369621/1‑149 (MQ=255)                                                                                                                                   tCGCCCGAGTCTCGGGTGATGAAACCAAAGCCTTTCGTCGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCAGGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcac                  <  5:382614/149‑1 (MQ=255)                                                                                                                                   tCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCGTTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcac                  >  6:19069/1‑149 (MQ=255)                                                                                                                                    cGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGccc                                <  4:325649/134‑1 (MQ=255)                                                                                                                                    cGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGccc                                >  3:325649/1‑134 (MQ=255)                                                                                                                                     gCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacg                 <  1:103302/148‑1 (MQ=255)                                                                                                                                      cccGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggc               >  4:207378/1‑149 (MQ=255)                                                                                                                                       ccGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggc               >  3:188052/1‑148 (MQ=255)                                                                                                                                       ccGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCCTGCTGCGGGCCAAGCCCACGCCGAGATGcacgga               >  6:369679/1‑147 (MQ=255)                                                                                                                                       ccGAGACTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc                                                    >  6:298835/1‑111 (MQ=255)                                                                                                                                       ccGAGACTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc                                                    <  5:298835/111‑1 (MQ=255)                                                                                                                                             ctcGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcacggccacggc         >  2:94533/1‑148 (MQ=255)                                                                                                                                                gggAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAgc     <  3:207378/149‑1 (MQ=255)                                                                                                                                                   agaTGAAACCAACGCCTTTCGACGTGTTGACCCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGGGCCTTGCGCGGGCTGCGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGcgcc  <  1:182716/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCGAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCGGATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGT‑GCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCACGGCCACGGCCAGCGCC  >  NC_002947/1387905‑1388199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑