| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,400,876 | T→G | 5.5% | intergenic (+39/‑130) | ybgF → / → queE | TolQRA transport system periplasmic protein/7‑carboxy‑7‑deazaguanine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,400,876 | 0 | T | G | 5.5% | 276.4 / 6.1 | 91 | intergenic (+39/‑130) | ybgF/queE | TolQRA transport system periplasmic protein/7‑carboxy‑7‑deazaguanine synthase |
| Reads supporting (aligned to +/- strand): ref base T (40/46); new base G (2/3); total (42/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
CGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGAC > NC_002947/1400730‑1401014 | cGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCGAGTcg > 7:221674/1‑149 (MQ=255) tGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCTCTCGAAAGAAACCCGCGCCAGTCGCgg < 7:289197/148‑1 (MQ=255) tGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCgg < 4:180527/148‑1 (MQ=255) ggCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGtt < 3:2061/149‑1 (MQ=255) ccATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAA‑AAACCCGCGACTGGCGCGGGtttct > 6:377012/1‑147 (MQ=255) aTACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGttt‑ttt > 8:290981/1‑149 (MQ=255) tACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTc < 3:180557/149‑1 (MQ=255) tACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGACTGGCGCGGGttt‑cttt < 5:243065/149‑2 (MQ=255) tACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAA‑AAACCCGCGACTGGCGCGGGttt‑ctttc < 5:377012/149‑3 (MQ=255) aCCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCg > 3:190050/1‑149 (MQ=255) ccGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCTAGTCGCGGGTTT‑TTTCGt < 8:221674/149‑1 (MQ=255) ccGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGt < 2:234498/149‑1 (MQ=255) cGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGt > 8:329513/1‑148 (MQ=255) gACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAA‑AAACCCGCGACTGGCGCGGGTTTCTTTCgagc < 8:134401/149‑4 (MQ=38) aaGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGa > 8:202068/1‑148 (MQ=255) ggTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATc < 4:18702/149‑1 (MQ=255) gcctagagcttctgcaggtcacgctgggccagttgagcggcagaggtgccggggtactgggtcacgacctgctgaagCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGc > 6:312288‑M2/78‑149 (MQ=255) aaGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGc < 7:83432/149‑1 (MQ=255) aGGCATCCTTCAGCAGGTCGTGACCCAATACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGcc > 2:128241/1‑149 (MQ=255) ggCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGccc > 2:360925/1‑149 (MQ=255) gCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCt > 7:18347/1‑149 (MQ=255) cttctgcaggtcacgctgggccagttgagcggcagaggtgccggggtactgggtcacgacctgctgaagCTCTAGGCGTTGTGGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGt > 1:296244‑M2/70‑121 (MQ=255) cttctgcaggtcacgctgggccagttgagcggcagaggtgccggggtactgggtcacgacctgctgaagCTCTAGGCGTTGTGGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGt < 2:296244‑M2/52‑1 (MQ=255) CTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCttttt > 2:315133‑M1/1‑148 (MQ=255) CTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGACCAGCGTGACCTGCAGAAGCTCTAGGCGGTGTCGCTGTACCGCGCGAAAGAAAACCGCGCCAGTCGCGACTTT‑TTTCGTTAGAATCACTGCACtttttt < 6:23859‑M1/149‑1 (MQ=255) ctgcaggtcacgctgggccagttgagcggcagaggtgccggggtactgggtcacgacctgctgaagCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTc < 5:312288‑M2/81‑1 (MQ=255) gcagGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTa > 4:71909/1‑148 (MQ=255) agGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTaaa > 5:136267/1‑148 (MQ=255) agGTCCGGACCCAGTACCCCGGCACCTCTGCCGCTCATCTGTCCCAGCGTGACGTGGAGAAGCTGTAGGCGTTGTCGCTGTTCCGCTCGAATGAATCCCGCGCCAGTCTCGGGTTT‑TTTCGTTAGATTCAGTGCCCTTTTTTCGTAAac < 2:289312/149‑1 (MQ=255) gTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAacac > 2:231994/1‑149 (MQ=255) gagACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCACTAGGCGTAGTCGCAGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTAT‑TTTCGTTAGAATCACTGCCCTTTTATCGTAAACACgcg > 5:272796/3‑148 (MQ=255) gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGttt‑ttt < 7:226113/114‑1 (MQ=255) gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGttt‑ttt > 8:226113/1‑114 (MQ=255) tgccccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTGTAGGCGTTGACGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTT‑TTGCGTTAGAATCACTGCCCTTTTTTCGTAAACACgctg > 5:80388/4‑149 (MQ=255) tGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCTCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACgctg < 7:166963/149‑1 (MQ=255) aCCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACgctgct > 7:173182/1‑149 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCttttt > 6:128273/1‑131 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCttttt < 5:128273/131‑1 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCtttt > 7:313314/1‑130 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCtttt < 8:313314/130‑1 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc > 3:172714/1‑149 (MQ=255) tACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGAt < 3:71909/149‑1 (MQ=255) aCCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGAt > 2:281566/1‑148 (MQ=255) cGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGc > 3:330715/1‑149 (MQ=255) ccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGt < 4:172714/149‑1 (MQ=255) ctctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTc > 5:262957/1‑149 (MQ=255) tctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTc > 3:141979/1‑148 (MQ=255) ccGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGa < 4:190050/146‑1 (MQ=255) ccGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTc > 4:173785/1‑149 (MQ=255) aaCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGa > 1:206761/1‑148 (MQ=255) aaCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGa > 5:340307/1‑148 (MQ=255) cTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAg < 2:122810/149‑1 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGt < 1:281566/147‑1 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTg > 4:326264/1‑148 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGc < 6:262957/149‑1 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGc < 7:329513/149‑1 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGc < 4:330715/149‑1 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGc < 8:18347/149‑1 (MQ=255) ccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCt > 3:239842/1‑149 (MQ=255) gTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGAACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTACACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACgg < 6:272796/149‑1 (MQ=255) tGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGa < 8:173182/149‑1 (MQ=255) tGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGa < 2:206761/149‑1 (MQ=255) aCCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAgg < 7:202068/149‑1 (MQ=255) ccTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAgg < 1:231994/148‑1 (MQ=255) cTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCcg < 2:225570/128‑1 (MQ=255) cTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCcg > 1:225570/1‑128 (MQ=255) tGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCg > 7:180896/1‑148 (MQ=255) cAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCgg < 3:326264/147‑1 (MQ=255) aGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGa > 2:262542/1‑147 (MQ=255) aagCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGc < 1:360925‑M2/146‑1 (MQ=255) ggCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGtt < 5:282389/146‑1 (MQ=255) ggCGTTGTCGCTGTACCGCTCGAAAGAAACCCCCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTa < 7:290981/148‑1 (MQ=255) ggCGTTGGCGCTGTACCGCTCGAACGAAACCCGCGCCAGTCGCGAGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGTCAGTGCCTGACGGAGGCGGACAGCCTGTTTAg < 5:23859/149‑1 (MQ=255) cGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCt < 6:136267/149‑1 (MQ=255) gTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATAACTGCCCATTTTTAGTAAAAACCCAGCTGCGGATAACGCAATTTCGAGTACGCGACAGTGCCTGAAGGACGCGGACAGCCTGTTTAGCTg > 2:190018/1‑149 (MQ=255) gTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTc < 1:262542/148‑1 (MQ=255) gCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAgg > 7:141189/1‑124 (MQ=255) gCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAgg < 8:141189/124‑1 (MQ=255) gCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGc < 1:128241/149‑1 (MQ=255) tACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGccc < 1:181332/147‑1 (MQ=255) cGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGc < 6:340307/149‑1 (MQ=255) cGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGc > 6:31981/1‑149 (MQ=255) cGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGc > 4:10800/1‑149 (MQ=255) cTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGc > 7:181838/1‑147 (MQ=255) tCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAg > 5:22791/1‑149 (MQ=255) acgaaaaaaCCCGCGACTGGCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAg > 3:314074/7‑148 (MQ=255) gaaagaaaCCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCa < 4:141979/149‑1 (MQ=255) aaagaaaCCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCAt > 2:288686/1‑149 (MQ=255) aaaCCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCaa < 1:190018/149‑1 (MQ=255) aCCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGa < 5:31981/149‑1 (MQ=255) cccGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGac < 6:22791/149‑1 (MQ=255) | CGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTT‑TTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGAC > NC_002947/1400730‑1401014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |