BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,339	T→A	6.6%	Q15L (CAG→CTG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,339	0	T	A	6.6%	299.3 / 7.0	102	Q15L (CAG→CTG)	trmE	GTPase
Reads supporting (aligned to +/- strand): ref base T (48/47); new base A (5/2); total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 102 total aligned reads displayed.

TCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTT  >  NC_002947/6193‑6484

tCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTgg                                                                                                                                                  <  7:2517/149‑1 (MQ=255)

tCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTgg                                                                                                                                                  >  2:244139/1‑149 (MQ=255)

tCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTgg                                                                                                                                                  <  2:85355/149‑1 (MQ=255)

  ccTTCTTCCAGCACCAGCCCGTCATCGTCGCGGAATGGACCGTAATGGGCATGACGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTggg                                                                                                                                                 <  8:349354/148‑1 (MQ=255)

  ccTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGc                                                                                                                                                >  7:349519/1‑149 (MQ=255)

       gTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTg                                                                                                                                            <  2:163576/148‑1 (MQ=255)

        tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGc                                                                                                                                                <  4:68219/143‑1 (MQ=255)

        tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGc                                                                                                                                                >  3:68219/1‑143 (MQ=255)

          cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                        >  2:351183/1‑148 (MQ=255)

          cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑a                                                                                                                                       >  2:200774/1‑149 (MQ=255)

               ccAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATggcg                                                                                                                                  <  1:58800/149‑1 (MQ=255)

                 aGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCACAGGGCCGGACAACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCG‑GTgtggga                                                                                                                                <  1:351183/149‑6 (MQ=255)

                      ttCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                          >  5:276478/1‑135 (MQ=255)

                      ttCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                          <  6:276478/135‑1 (MQ=255)

                      ttCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATgg                                                                                                                           >  8:249247/1‑149 (MQ=255)

                            gtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCa                                                                                                                      <  6:48230/148‑1 (MQ=255)

                               gcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGca                                                                                                                  <  4:35957/149‑1 (MQ=255)

                                     tGGACGGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGGCGGCCTTGGCAGCCAGAGGGGCGGACAACCTGACAATTCCCACTCCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAgtg                                                                                                             <  5:380106/148‑1 (MQ=255)

                                     tGGACCGTAATGGGCATGCCGCGGGGTCAGGGTCCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAAATCCCACACCGCCCCGCCCCTGGGCCCTGGCG‑ATGGCCGCGATGGTTTAACGCACAgtg                                                                                                             >  6:380106/1‑148 (MQ=255)

                                       gACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                          <  1:357334/118‑1 (MQ=255)

                                       gACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                          >  2:357334/1‑118 (MQ=255)

                                        aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACACCCTGACAATTCCCACACCGCTCCGGCCCTGGGCCGTGGCG‑a                                                                                                                                       >  8:122598/1‑119 (MQ=255)

                                         ccGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                        >  7:225973/1‑149 (MQ=255)

                                           gTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGc                                                                                                      <  5:218125/149‑1 (MQ=255)

                                            tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTGCATGCt                                                                                                     >  6:66759/1‑149 (MQ=255)

                                                    aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                             >  7:159700/1‑149 (MQ=255)

                                                      gCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc                                                                                           >  2:89778/1‑149 (MQ=255)

                                                      gCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGGGTTAATGCTAGAAAGCct                                                                                            >  6:265043/1‑148 (MQ=255)

                                                       ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc                                                                                           >  2:29963/1‑148 (MQ=255)

                                                        cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                >  7:261215/1‑142 (MQ=255)

                                                        cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                <  8:261215/142‑1 (MQ=255)

                                                          cgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGTATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                        <  1:89778/149‑1 (MQ=255)

                                                              gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGcaca                                                                                                                >  5:85255/1‑120 (MQ=255)

                                                              gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGcaca                                                                                                                <  6:85255/120‑1 (MQ=255)

                                                              gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaa                                                                                   <  1:244139/149‑1 (MQ=255)

                                                               tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATg                                                                                                       <  6:286624/128‑1 (MQ=255)

                                                               tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATg                                                                                                       >  5:286624/1‑128 (MQ=255)

                                                                 aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATg                                                                                                                            >  7:279679/1‑105 (MQ=255)

                                                                 aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATg                                                                                                                            <  8:279679/105‑1 (MQ=255)

                                                                 aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCG‑GTgt                                                                                                                                    <  5:366961/97‑2 (MQ=38)

                                                                 aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCG‑GTgt                                                                                                                                    >  6:366961/1‑96 (MQ=38)

                                                                    gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCct                                                                                            >  5:211806/1‑134 (MQ=255)

                                                                    gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCct                                                                                            <  6:211806/134‑1 (MQ=255)

                                                                    gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                             <  1:375241/149‑1 (MQ=255)

                                                                     tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                            <  8:159700/149‑1 (MQ=255)

                                                                      gCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                           <  8:349519/149‑1 (MQ=255)

                                                                             gTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCTGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGc                                                                     >  2:178002/1‑148 (MQ=255)

                                                                               gATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaa                                                                   >  4:297194/1‑148 (MQ=255)

                                                                                   aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACg                                                              >  3:333581/1‑149 (MQ=255)

                                                                                   aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCCATGGTTTCACGTACAGTGTTCATGCTCGAAAGCCTCTACGAAAAAAACGACAGAAACCAAAAACg                                                              >  6:345657/1‑149 (MQ=255)

                                                                                    aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                             <  6:16532/149‑1 (MQ=255)

                                                                                      aGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGccc                                                           <  8:225973/149‑1 (MQ=255)

                                                                                        gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTTGCG‑ATGGCGGCGATGGTTTCACGCACAgtgt                                                                                                            <  1:247271/98‑1 (MQ=255)

                                                                                        gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTTGCG‑ATGGCGGCGATGGTTTCACGCACAgtgt                                                                                                            >  2:247271/1‑98 (MQ=255)

                                                                                        gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                         >  4:315827/1‑149 (MQ=255)

                                                                                        gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                         >  3:280251/1‑149 (MQ=255)

                                                                                        gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                         >  5:37084/1‑149 (MQ=255)

                                                                                        gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTAATGCTCGAAAGCCTCGACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                         >  1:241299/1‑149 (MQ=255)

                                                                                          cagccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCAc                                                        >  3:181726/3‑148 (MQ=255)

                                                                                                      ccAGAGGGCCGGACAACCTGACAATTCCCACCCCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                           <  5:66759/149‑1 (MQ=255)

                                                                                                      ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGccc                                                           >  2:218239/1‑133 (MQ=255)

                                                                                                      ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGccc                                                           <  1:218239/133‑1 (MQ=255)

                                                                                                      ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                           >  2:329517/1‑149 (MQ=255)

                                                                                                        atatggCCGGACAAACTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACATTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATACCAAAAACGCCCCACTAGGGGGCGtttttt                                         <  4:181726/145‑1 (MQ=255)

                                                                                                           gggCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCTCAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTAtt                                      <  1:200774/149‑1 (MQ=255)

                                                                                                            ggCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                         <  6:83988/129‑1 (MQ=255)

                                                                                                            ggCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                         >  5:83988/1‑129 (MQ=255)

                                                                                                              ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCAGGGCCGTGGCG‑ATGGCGGCGATCGTTTCACGCACACTGTTCATGCTCGAAAGCCTCAACGACAAAAACGACAGATAGAAAAAACGCCCCACTAGGGGGCGTTTTTTAttaa                                    >  5:64372/1‑146 (MQ=255)

                                                                                                                ggACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAg                                 <  1:29963/149‑1 (MQ=255)

                                                                                                                  gcAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGc                               >  5:346620/2‑149 (MQ=255)

                                                                                                                    aaCCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTa                             <  7:249247/149‑1 (MQ=255)

                                                                                                                      ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCa                                                                                                                      >  8:170584/1‑58 (MQ=255)

                                                                                                                      ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCa                                                                                                                      <  7:170584/58‑1 (MQ=255)

                                                                                                                      ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGc                           >  4:93600/1‑149 (MQ=255)

                                                                                                                       cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                          >  1:42971/1‑149 (MQ=255)

                                                                                                                       cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                          >  4:108720/1‑149 (MQ=255)

                                                                                                                        tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                            <  6:71269/114‑1 (MQ=255)

                                                                                                                        tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                            >  5:71269/1‑114 (MQ=255)

                                                                                                                        tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                          >  5:227810/1‑148 (MQ=255)

                                                                                                                        tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGt                         >  7:225539/1‑149 (MQ=255)

                                                                                                                              ttCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc                   <  8:225539/149‑1 (MQ=255)

                                                                                                                                 ccACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc                 <  4:280251/148‑1 (MQ=255)

                                                                                                                                 ccACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                >  8:71309/1‑149 (MQ=255)

                                                                                                                                  cacaCCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt               <  6:227810/149‑1 (MQ=255)

                                                                                                                                  cacaCCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt               >  1:230607/1‑149 (MQ=255)

                                                                                                                                   ccatcGCCACGGCCCAGGGCCGGGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt               >  1:139302/5‑148 (MQ=255)

                                                                                                                                     acCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc              >  2:62228/1‑147 (MQ=255)

                                                                                                                                      tcGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTa                     <  1:278050/138‑1 (MQ=255)

                                                                                                                                      tcGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTa                     >  2:278050/2‑139 (MQ=255)

                                                                                                                                      ccGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTa                                                      >  6:282752/1‑106 (MQ=255)

                                                                                                                                      ccGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTa                                                      <  5:282752/106‑1 (MQ=255)

                                                                                                                                      ccGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGt                         >  2:112214/1‑135 (MQ=255)

                                                                                                                                      ccGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGt                         <  1:112214/135‑1 (MQ=255)

                                                                                                                                      ccGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag           <  3:93600/149‑1 (MQ=255)

                                                                                                                                      ccGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag           <  1:329517/149‑1 (MQ=255)

                                                                                                                                         ccccGGCCCTGGGCTGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag        <  1:178002/149‑1 (MQ=255)

                                                                                                                                         ccacGGCCCAGGGCCGGGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag        >  2:120321/4‑149 (MQ=255)

                                                                                                                                          cccGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc       <  6:37084/149‑1 (MQ=255)

                                                                                                                                             ggCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagcagc    <  7:77428/149‑1 (MQ=255)

                                                                                                                                               cccTGGGCCGTTGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCGTCTACGACAAAAACGACAGATAGCAAATTCGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtt  <  5:304874/149‑1 (MQ=255)

TCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCG‑ATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTT  >  NC_002947/6193‑6484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`