Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,453,974	C→G	5.8%	A203G (GCC→GGC)	PP_1272 →	multidrug MFS transporter membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,453,974	0	C	G	5.8%	225.2 / 3.1	69	A203G (GCC→GGC)	PP_1272	multidrug MFS transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base G (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CTTGCCGAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCGC  >  NC_002947/1453826‑1454116                                                                                                                                                     |                                                                                                                                                cTTGCCGAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAgcgc                                                                                                                                                 <  5:63414/149‑1 (MQ=255)   tGCCGAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGcc                                                                                                                                                >  7:88952/1‑148 (MQ=255)     ccGAGCAGGGCGCGGTTACCGTGCAGGACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑At                                                                                                                                            >  1:5541/1‑149 (MQ=255)     ccGAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑At                                                                                                                                            <  1:282984/149‑1 (MQ=255)       gAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑a                                                                                                                                             <  3:26869/146‑1 (MQ=255)       gAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑a                                                                                                                                             >  4:26869/1‑146 (MQ=255)           aGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAg                                                                                                                                      >  4:215633/1‑149 (MQ=255)              gcgcGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTc                                                                                                                                   <  8:280950/149‑1 (MQ=255)                gcgGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGACCATCGGCG‑CTGGCCacttgag                                                                                                                                 <  5:40597/149‑7 (MQ=255)                  gggTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCG‑CTGGCCactt                                                                                                                                    <  1:351320/144‑4 (MQ=255)                  gggTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCG‑CTGGCCactt                                                                                                                                    >  2:351320/1‑141 (MQ=255)                          tGCAGAACACCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCg                                                                                                                         >  8:137367/1‑147 (MQ=255)                           gCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCt                                                                                                                                    >  5:273539/1‑135 (MQ=255)                           gCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCt                                                                                                                                    <  6:273539/135‑1 (MQ=255)                            cAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGc                                                                                                                     <  4:76923/149‑1 (MQ=255)                              gAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAg                                                                                                                   >  5:30406/1‑149 (MQ=255)                                   cccAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTg                                                                                                              <  6:297236/149‑1 (MQ=255)                                       gcagGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTg                                                                                                              <  2:365846/145‑1 (MQ=255)                                       gcagGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTg                                                                                                              >  1:365846/1‑145 (MQ=255)                                        cagGAGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGaa                                                                                                           >  6:42096/1‑147 (MQ=255)                                         agGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAg                                                                                                        <  6:359258/149‑1 (MQ=255)                                          ggCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAgg                                                                                                       <  8:77706/149‑1 (MQ=255)                                            cgcgcAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGcc                                                                                                     >  1:55426/1‑149 (MQ=255)                                             gcgcAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGccc                                                                                                    <  6:275400/149‑1 (MQ=255)                                                     cTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGAt                                                                                            <  7:63788/149‑1 (MQ=255)                                                          gATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTc                                                                                       >  4:232051/1‑149 (MQ=255)                                                             cAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGAt                                                                                            <  3:4318/141‑1 (MQ=255)                                                             cAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGAt                                                                                            >  4:4318/1‑141 (MQ=255)                                                               aGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTaca                                                                                  >  7:45033/1‑149 (MQ=255)                                                                   cGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCg                                                                              <  2:82691/149‑1 (MQ=255)                                                                     tGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCa                                                                            <  8:88952/149‑1 (MQ=255)                                                                          gCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATca                                                                         <  7:273112/147‑1 (MQ=255)                                                                          gCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTCTCCTACACCCGCATcaca                                                                       <  1:361180/149‑1 (MQ=255)                                                                                  aaCGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGt                                                               <  5:42096/149‑1 (MQ=255)                                                                                  aaCGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGt                                                               >  8:285219/1‑149 (MQ=255)                                                                                  aaCGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCAAGCTCGATCTGTCATACACCCGCATACCAGCGCCGGt                                                               >  3:235633/1‑149 (MQ=255)                                                                                     gCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGa                                                            <  7:285219/149‑1 (MQ=255)                                                                                       tCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCaa                                                                                                                                 >  6:390147/1‑78 (MQ=255)                                                                                         aaGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGTCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACgg                                                         >  2:237754/1‑148 (MQ=255)                                                                                         aaGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACgg                                                         >  1:26658/1‑148 (MQ=255)                                                                                                 cgGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCgg                                                <  3:232051/149‑1 (MQ=255)                                                                                                      ggcggcGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGAATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGaa                                            >  1:284579/1‑149 (MQ=255)                                                                                                      ggcggcGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑TTGGCCAGCTGAAGCGTGCCGAAGCAGGCCTGGAATCGGCCCATCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTTGACGGTATGGTCGGCGAAc                                           <  8:152413/149‑1 (MQ=255)                                                                                                       gcggcgCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAAcg                                          >  2:58203/1‑149 (MQ=255)                                                                                                               cAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGt                                                               >  7:282599/1‑120 (MQ=255)                                                                                                               cAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGt                                                               <  8:282599/120‑1 (MQ=255)                                                                                                                  gCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGaa                                                                                                           <  5:327374/73‑1 (MQ=255)                                                                                                                  gCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGaa                                                                                                           >  6:327374/1‑73 (MQ=255)                                                                                                                      gTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTggg                           <  2:55426/149‑1 (MQ=255)                                                                                                                         gACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGc                        >  5:268214/1‑149 (MQ=255)                                                                                                                            aTCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTa                     >  6:218010/1‑149 (MQ=255)                                                                                                                             tCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTAc                    >  8:36824/1‑149 (MQ=255)                                                                                                                              ccTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTc                                                  >  7:175003/1‑118 (MQ=255)                                                                                                                              ccTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTc                                                  <  8:175003/118‑1 (MQ=255)                                                                                                                                tCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGt                  >  1:99670/1‑148 (MQ=255)                                                                                                                                tCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGt                  >  3:140255/1‑148 (MQ=255)                                                                                                                                    ggCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAAcc             >  4:88467/1‑149 (MQ=255)                                                                                                                                    ggCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAAcc             >  4:1711/1‑149 (MQ=255)                                                                                                                                       tCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCgg          >  5:109815/1‑149 (MQ=255)                                                                                                                                        gagctGGCCATCGGCG‑CTGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCggg         >  1:221274/5‑149 (MQ=255)                                                                                                                                         aaGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCggg         <  7:180179/148‑1 (MQ=255)                                                                                                                                         aaGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCgg          <  1:206517/147‑1 (MQ=255)                                                                                                                                         aaGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCgg          <  4:140255/147‑1 (MQ=255)                                                                                                                                         aaGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGgc        >  2:89236/1‑149 (MQ=255)                                                                                                                                         aaGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGgc        <  2:284579/149‑1 (MQ=255)                                                                                                                                            tGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGgcgc      >  4:87198/1‑148 (MQ=255)                                                                                                                                            tGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGgcgc      <  3:87198/148‑1 (MQ=255)                                                                                                                                            tGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCt     >  8:189815/1‑149 (MQ=255)                                                                                                                                              gCCAGCGCCG‑ATGGTCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCg   <  1:237754/149‑1 (MQ=255)                                                                                                                                              gCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCCACCCGGGCGCTCg   >  8:64214/1‑149 (MQ=255)                                                                                                                                               ccAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCGc  <  2:308132/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                CTTGCCGAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCG‑ATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCGC  >  NC_002947/1453826‑1454116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑