Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,455,474 G→C 5.5% S336S (TCG→TCC)  PP_1273 → multidrug MFS transporter outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,455,4740GC 5.5% 236.2 / 3.3 73S336S (TCG→TCC) PP_1273multidrug MFS transporter outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GTCCGAACTGGCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATCGCAAGGTGGTGCTGG  >  NC_002947/1455327‑1455622
                                                                                                                                                   |                                                                                                                                                    
gTCCGAACTGGCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCgg                                                                                                                                                     <  6:141888/149‑1 (MQ=255)
  ccGAACTGGCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGAt                                                                                                                                                   >  6:245958/1‑149 (MQ=255)
   cGAACTGGCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCTAGGCCCTGCAGCTGTCGGAtt                                                                                                                                                  <  4:41447/149‑1 (MQ=255)
     aaCTGGCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTc                                                                                                                                                <  3:118812/149‑1 (MQ=255)
         ggCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCTCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCg                                                                                                                                               <  5:320602/146‑1 (MQ=255)
         ggCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCg                                                                                                                                               >  6:320602/1‑146 (MQ=255)
          gCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCGAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTgg                                                                                                                                           >  1:104995/1‑149 (MQ=255)
          gCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTgg                                                                                                                                           >  6:285919/1‑149 (MQ=255)
            cGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCt                                                                                                                                         <  7:155865/149‑1 (MQ=255)
             gAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTg                                                                                                                                        >  5:114395/1‑149 (MQ=255)
                   cgccCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTc                                                                                                                                  >  1:170238/1‑149 (MQ=255)
                     cccGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTcgc                                                                                                                                >  7:220585/1‑149 (MQ=255)
                     cccGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTcgc                                                                                                                                <  7:15813/149‑1 (MQ=255)
                            aTCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCTGATTTCGGTGGCTGGGATTCGCGCCGGtt                                                                                                                         <  4:170651/149‑1 (MQ=255)
                              ccGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTg                                                                                                                       >  6:217099/1‑149 (MQ=255)
                                gCCAGGCCGAGGCCCGCCTGCATTCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTgg                                                                                                                                       <  1:114338/131‑1 (MQ=255)
                                gCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTgg                                                                                                                                       >  2:114338/1‑131 (MQ=255)
                                gCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGcc                                                                                                                     >  6:332824/1‑149 (MQ=255)
                                  cAGGCCGAGGCCGGCCTGCATGCCGCCACCTCCAGCATTGGGGTGGCCAAGGCGGATTTTTACCCCCGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCtt                                                                                                                   <  8:286124/149‑1 (MQ=255)
                                  cAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCTGATTTTTACCCCAGCCTCCGCCTGTCGGGCAGTGGCGGCTTCCAGGCCCTGCAGCTGGCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCtt                                                                                                                   <  4:93411/149‑1 (MQ=255)
                                    ggCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCg                                                                                                                 <  5:245958/149‑1 (MQ=255)
                                      ccGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGt                                                                                                               <  2:170238/149‑1 (MQ=255)
                                      ccGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGt                                                                                                               >  2:160534/1‑149 (MQ=255)
                                       cGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTc                                                                                                              >  8:174926/1‑149 (MQ=255)
                                       cGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTc                                                                                                              >  4:225023/1‑149 (MQ=255)
                                         aGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCTGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCca                                                                                                            >  1:266723/1‑149 (MQ=255)
                                          ggCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTccc                                                                                                            >  5:59237/1‑147 (MQ=255)
                                           gCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCcaca                                                                                                          <  2:104995/149‑1 (MQ=255)
                                            cccGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAg                                                                                                         <  1:160534/149‑1 (MQ=255)
                                                ccTGCATGCCGCCACCGCCAGCATTGGCGTGGCCTAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCATCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGt                                                                                                     <  7:17317/149‑1 (MQ=255)
                                                      tGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGc                                                                                               <  3:140481/149‑1 (MQ=255)
                                                       gccgccACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGcc                                                                                              >  6:107377/1‑149 (MQ=255)
                                                         cgccaccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGa                                                                                            <  6:114395/149‑1 (MQ=255)
                                                         cgccaccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCCGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCcaca                                                                                                          <  6:95859/135‑1 (MQ=255)
                                                         cgccaccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCCGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCcaca                                                                                                          >  5:90401/1‑135 (MQ=255)
                                                         cgccaccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCCGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCcaca                                                                                                          >  5:95859/1‑135 (MQ=255)
                                                         cgccaccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCCGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCcaca                                                                                                          <  6:90401/135‑1 (MQ=255)
                                                         cgccaccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCGGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGAGTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGa                                                                                            >  5:377398/1‑149 (MQ=255)
                                                            caccgccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGAttt                                                                                         <  5:285919/149‑1 (MQ=255)
                                                             accgccaGCATTGGCGTGGCCAAGGCGTATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGAtttt                                                                                        <  3:291191/149‑1 (MQ=255)
                                                                 ccaGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGaa                                                                                    <  8:220585/149‑1 (MQ=255)
                                                                   aGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCACCAGTCGGATTTCGGTCGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAgg                                                                                  >  7:331952/1‑149 (MQ=255)
                                                                    gCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGt                                                                                 <  5:107377/149‑1 (MQ=255)
                                                                      aTTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTgg                                                                               <  6:249257/149‑1 (MQ=255)
                                                                         ggCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGCAGGTGGcc                                                                             <  6:377398/148‑1 (MQ=255)
                                                                          gCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGccctt                                                                           >  7:187490/1‑147 (MQ=255)
                                                                            gTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCt                                                                         >  3:76058/1‑149 (MQ=255)
                                                                                ccAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAg                                                                     >  6:48683/1‑149 (MQ=255)
                                                                                           ttttACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTgg                                                           >  8:320473/1‑148 (MQ=255)
                                                                                                 cccAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGc                                                                                                  >  8:330883/1‑103 (MQ=255)
                                                                                                 cccAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGc                                                                                                  <  7:330883/103‑1 (MQ=255)
                                                                                                      cATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAgg                                               >  5:83894/1‑149 (MQ=255)
                                                                                                          cGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGgcgc                                            <  7:174926/148‑1 (MQ=255)
                                                                                                           gCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCaa                                          >  7:266485/1‑149 (MQ=255)
                                                                                                                tCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAAcagca                                     <  5:332824/149‑1 (MQ=255)
                                                                                                                tCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAAcagc                                      <  3:225023/148‑1 (MQ=255)
                                                                                                                  gggCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAgg                                   <  7:320473/149‑1 (MQ=255)
                                                                                                                   ggCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAgg                                   >  3:278150/1‑148 (MQ=255)
                                                                                                                   gccAGTGTCGGCTTCCACGCCCTGCACCTGTCGGATTTCGCTGGCAGCGATTCGCGCCGCTTTGCCTTCCGTCCACACATGTCGCTGCCGATTTTCGAAAGTGGCCGGCTCAAAGCCACCCTGGAACTGCCCGAGGCGCAAcagcacg                                   >  8:104505/3‑146 (MQ=255)
                                                                                                                    gCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGa                                  <  3:202953/148‑1 (MQ=255)
                                                                                                                    gCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGa                                  >  4:202953/1‑148 (MQ=255)
                                                                                                                          tCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCcgcg                           >  2:192941/1‑149 (MQ=255)
                                                                                                                             gCTTCGAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCt                         >  7:347235/1‑148 (MQ=255)
                                                                                                                               ttCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGaa                      >  4:371726/1‑149 (MQ=255)
                                                                                                                                tCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAAc                     <  3:315081/149‑1 (MQ=255)
                                                                                                                                 ccAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCCCGAGGCGCAACAGCAGGAAGCCGCGCTGAAc                     >  6:269390/1‑148 (MQ=255)
                                                                                                                                  cAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCg                                                                                                   >  3:140398/1‑69 (MQ=255)
                                                                                                                                  cAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCg                                                                                                   <  4:140398/69‑1 (MQ=255)
                                                                                                                                  cAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACt                    >  2:116386/1‑148 (MQ=255)
                                                                                                                                   aGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTAt                  <  5:217099/149‑1 (MQ=255)
                                                                                                                                    ggCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATc                 <  2:266723/149‑1 (MQ=255)
                                                                                                                                    ggCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATc                 <  1:116386/149‑1 (MQ=255)
                                                                                                                                        cTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATCGc               >  7:129245/1‑147 (MQ=255)
                                                                                                                                                 tCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATCGCAAGGTGGTGCt    >  1:368336/1‑149 (MQ=255)
                                                                                                                                                   ggATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATCGCAAGGTGGTGCTgg  <  8:347235/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCCGAACTGGCCGAGCGCCGCCCGGACATCCGCCAGGCCGAGGCCCGCCTGCATGCCGCCACCGCCAGCATTGGCGTGGCCAAGGCGGATTTTTACCCCAGCATCCGCCTGTCGGGCAGTGTCGGCTTCCAGGCCCTGCAGCTGTCGGATTTCGGTGGCTGGGATTCGCGCCGGTTTGCCTTCGGTCCACAGATGTCGCTGCCGATTTTCGAAGGTGGCCGGCTCAAGGGCACCCTGGAACTGCGCGAGGCGCAACAGCAGGAAGCCGCGCTGAACTATCGCAAGGTGGTGCTGG  >  NC_002947/1455327‑1455622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑