Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,457,149	T→C	11.6%	R110R (CGA→CGG)	PP_1275 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,457,149	0	T	C	11.6%	149.2 / 9.2	60	R110R (CGA→CGG)	PP_1275	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/22);  new base C (3/4);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CACATCAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGACAGTGTTCGGGG  >  NC_002947/1457010‑1457296                                                                                                                                            |                                                                                                                                                    cacaTCACTTGGCAAACGTACCTTGCAGGTGCTCGGCGCGGAATTCCAGGGGCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCGGCAATTCACCTGGCCCGCGATACCGCgggcca                                                                                                                                            <  6:230617/149‑4 (MQ=255)    aTCAATTGGCAAACGTACCTTGCAGGTGCTCTGCTCGGAATTCCAGGGTCTGCGGACGATAGCCTGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGtt                                                                                                                                          >  1:6134/1‑148 (MQ=255)     tCAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTg                                                                                                                                         >  7:285373/1‑148 (MQ=255)     tCAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTg                                                                                                                                         <  8:285373/148‑1 (MQ=255)      cAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGtt                                                                                                                                          <  3:355855/146‑1 (MQ=255)      cAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGtt                                                                                                                                          <  3:350142/146‑1 (MQ=255)      cAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGtt                                                                                                                                          >  4:355855/1‑146 (MQ=255)      cAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGtt                                                                                                                                          >  4:350142/1‑146 (MQ=255)        aTTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAgg                                                                                                                                     >  3:24520/1‑149 (MQ=255)        aTTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGCCCCGCGATACCGCgggcc                                                                                                                                             <  2:346975/141‑3 (MQ=255)        aTTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGCCCCGCGATACCGCgggcc                                                                                                                                             <  6:345429/141‑3 (MQ=255)        aTTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGCCCCGCGATACCGCgggcc                                                                                                                                             >  1:346975/1‑139 (MQ=255)        aTTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGCCCCGCGATACCGCgggcc                                                                                                                                             >  5:345429/1‑139 (MQ=255)              aaaCGTACCTTGCGGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGATACCGCgggccag                                                                                                                                           <  4:218497/137‑5 (MQ=255)                          cAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCa                                                                                                                   >  2:332180/1‑149 (MQ=255)                            ggTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACg                                                                                                                 <  2:125458/149‑1 (MQ=255)                                 tcggctcggAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATc                                                                                                            >  1:146990/1‑149 (MQ=255)                                 tcggctcggAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATc                                                                                                            >  2:221384/1‑149 (MQ=255)                                    gctcggAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGcc                                                                                                         <  4:24520/149‑1 (MQ=255)                                      tcggAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCg                                                                                                           >  6:265857/1‑145 (MQ=255)                                      tcggAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCg                                                                                                           <  5:265857/145‑1 (MQ=255)                                            ttCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCAc                                                                                                                  <  2:251566/132‑1 (MQ=255)                                            ttCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCAc                                                                                                                  >  1:251566/1‑132 (MQ=255)                                               cAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTAcg                                                                                              >  6:291324/1‑149 (MQ=255)                                                 gggTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTAcgcc                                                                                            >  8:214030/1‑149 (MQ=255)                                                  ggTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGt                                                                                                     <  5:369202/139‑1 (MQ=255)                                                  ggTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGt                                                                                                     >  6:369202/1‑139 (MQ=255)                                                  ggTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTAcgccg                                                                                           >  7:65389/1‑149 (MQ=255)                                                                 gCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAgcg                                                                              >  1:315408/1‑147 (MQ=255)                                                                  ccGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAg                                                                           >  6:191113/1‑149 (MQ=255)                                                                    ggAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGtt                                                                         <  2:315408/149‑1 (MQ=255)                                                                     gAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTc                                                                        <  2:146990/149‑1 (MQ=255)                                                                         gCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAg                                                                           <  3:74578/142‑1 (MQ=255)                                                                         gCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAg                                                                           >  4:74578/1‑142 (MQ=255)                                                                                  gcAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCAcg                                                           >  7:180591/1‑149 (MQ=255)                                                                                          aggcagTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGcc                                                   >  5:93555/1‑149 (MQ=255)                                                                                              agTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGttc                                               <  8:65389/149‑1 (MQ=255)                                                                                              agTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGttc                                               >  7:69481/1‑149 (MQ=255)                                                                                               gTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGttct                                              <  1:332180/149‑1 (MQ=255)                                                                                                 cTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGttctt                                             >  2:63242/1‑148 (MQ=255)                                                                                                  ttGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTtc                                                                                                                                >  8:50325/1‑64 (MQ=255)                                                                                                  ttGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTtc                                                                                                                                <  7:50325/64‑1 (MQ=255)                                                                                                    gCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCACAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATg                                         >  1:335889/1‑149 (MQ=255)                                                                                                     ccACTCGGGCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCGGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGc                                        <  2:6134/149‑1 (MQ=255)                                                                                                     ccACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATg                                         <  1:221384/148‑1 (MQ=255)                                                                                                           ggCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGTGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTAc                                    <  5:390951/147‑1 (MQ=255)                                                                                                           ggCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCt                                  >  1:219374/1‑149 (MQ=255)                                                                                                           ggCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCt                                  >  3:141519/1‑149 (MQ=255)                                                                                                                    cTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAggagc                         >  4:170766/1‑146 (MQ=255)                                                                                                                     tGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACg                        >  3:223082/1‑149 (MQ=255)                                                                                                                        aaTTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTg                                                     >  6:322340/1‑117 (MQ=255)                                                                                                                        aaTTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTg                                                     <  5:322340/117‑1 (MQ=255)                                                                                                                            cACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTcggcg                 >  8:213527/1‑149 (MQ=255)                                                                                                                             aCCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTcggcgg                >  2:216110/1‑149 (MQ=255)                                                                                                                              aacgccccGCGATACCGC‑GGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGa              >  6:178440/6‑149 (MQ=255)                                                                                                                               cTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGa              <  6:93555/149‑1 (MQ=255)                                                                                                                               cTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGa              <  5:191113/149‑1 (MQ=255)                                                                                                                                  gCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGACa            <  8:180591/148‑1 (MQ=255)                                                                                                                                   cccGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGACAgt          <  8:251465/149‑1 (MQ=255)                                                                                                                                      gcgGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGACAGTGtt       >  6:367665/1‑149 (MQ=255)                                                                                                                                            tCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGACAGTGTTCgggg  <  7:214030/148‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                    CACATCAATTGGCAAACGTACCTTGCAGGTGCTCGGCTCGGAATTCCAGGGTCTGCGGCCGATAGCCGGAACGCAGCGGCGGCAATGGCAGGCAGTCTTGCCACTCGGCCCCCGGCTGCAATTCACCTGGCCCGCGGTATCGCGGGGCGTTGTAGGTGTTCTCGGCCAGGTTCACGGTATCGCCCGGTGCGTACGCCGCCACGCGCCAGCGCAGTTCGGTCAGCGGCACGTCGTTGCCGTTCTTCATGCGTACCTGCAAGGCACGGTCGGCGGGACAGTGTTCGGGG  >  NC_002947/1457010‑1457296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑