Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,469,808 A→G 13.0% V112A (GTG→GCG)  algK ← alginate biosynthesis protein AlgK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,469,8080AG13.0% 108.1 / 7.9 46V112A (GTG→GCG) algKalginate biosynthesis protein AlgK
Reads supporting (aligned to +/- strand):  ref base A (22/18);  new base G (3/3);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CGCGTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTC  >  NC_002947/1469663‑1469956
                                                                                                                                                 |                                                                                                                                                     
cgcgTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGccac                                                                                                                                                      >  4:320392/1‑147 (MQ=255)
              ggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAgg                                                                                                                                      >  1:21100/1‑149 (MQ=255)
               gAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGgc                                                                                                                                     >  2:92828/1‑149 (MQ=255)
                      tGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTg                                                                                                                             >  1:37889/1‑149 (MQ=255)
                        cGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGccac                                                                                                                                                      >  6:328422/1‑123 (MQ=255)
                         gggTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTGGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑Ctgcggc                                                                                                                          >  1:78414/1‑144 (MQ=255)
                               cTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGGGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTgg                                                                                                                    <  5:260144/149‑1 (MQ=255)
                                  aGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGc                                                                                                                   <  4:129683/147‑1 (MQ=255)
                                   ggTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGAc                                                                                                                >  7:246162/1‑149 (MQ=255)
                                            ttGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGcc                                                                                                       >  6:278526/1‑149 (MQ=255)
                                              gCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCg                                                                                                      <  1:244188/148‑1 (MQ=255)
                                               ccAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGc                                                                                                     <  2:37889/148‑1 (MQ=255)
                                                cAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCACGCGCCCAA‑GCGCGCCTTGGCCGCCTg                                                                                                                     >  1:325119/1‑131 (MQ=255)
                                                cAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCACGCGCCCAA‑GCGCGCCTTGGCCGCCTg                                                                                                                     <  2:325119/131‑1 (MQ=255)
                                                                ttGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTc                                                                                   <  3:329701/149‑1 (MQ=255)
                                                                   cTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCgg                                                                                 <  1:150056/148‑1 (MQ=255)
                                                                   cTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGc                                                                                >  3:244512/1‑149 (MQ=255)
                                                                      tGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTc                                                                                   <  4:198703/143‑1 (MQ=255)
                                                                      tGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTc                                                                                   >  3:198703/1‑143 (MQ=255)
                                                                         cccTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCtt                                                                          >  3:325912/1‑149 (MQ=255)
                                                                           cTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGa                                                                        >  1:345946/1‑149 (MQ=255)
                                                                              tttCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGAttt                                                                     >  4:308250/1‑149 (MQ=255)
                                                                                  gcgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGc                                                                   >  8:148082/1‑147 (MQ=255)
                                                                                        tGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTc                                                           >  3:68180/1‑149 (MQ=255)
                                                                                             gAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCgg                                                       <  3:320392/148‑1 (MQ=255)
                                                                                              aGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                     <  7:148082/149‑1 (MQ=255)
                                                                                               gCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTg                                                    <  8:246162/149‑1 (MQ=255)
                                                                                                 aGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGc                                                  >  1:373599/1‑149 (MQ=255)
                                                                                                        cGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCGCCTGCTGGTGGCCAAgc                                                                                                                                      <  6:123891/59‑2 (MQ=25)
                                                                                                        cGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCGCCTGCTGGTGGCCAAgc                                                                                                                                      >  5:123891/1‑58 (MQ=25)
                                                                                                          gCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGAt                                         <  3:308250/149‑1 (MQ=255)
                                                                                                               cTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTcc                                     <  5:278526/148‑1 (MQ=255)
                                                                                                                       cGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGcc                             >  8:99475/1‑148 (MQ=255)
                                                                                                                       cGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGcc                             <  2:373599/148‑1 (MQ=255)
                                                                                                                         gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTcc                                     <  3:130215/138‑1 (MQ=255)
                                                                                                                         gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTcc                                     >  4:130215/1‑138 (MQ=255)
                                                                                                                         gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGAc                          >  1:101983/1‑149 (MQ=255)
                                                                                                                         gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGAc                          >  4:375430/1‑149 (MQ=255)
                                                                                                                          ccTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACt                         >  5:325423/1‑149 (MQ=255)
                                                                                                                                  cTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCtt                  <  2:21100/148‑1 (MQ=255)
                                                                                                                                     tCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                     >  2:172749/1‑110 (MQ=255)
                                                                                                                                     tCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                     <  1:172749/110‑1 (MQ=255)
                                                                                                                                       cGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTg             >  4:5622/1‑148 (MQ=255)
                                                                                                                                        ggCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTa           <  4:244512/149‑1 (MQ=255)
                                                                                                                                        gccTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCg                                                                                  >  5:303232/3‑78 (MQ=37)
                                                                                                                                        gccTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCg                                                                                  <  6:303232/76‑1 (MQ=37)
                                                                                                                                          cTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGc                              <  2:377157/128‑1 (MQ=255)
                                                                                                                                          cTTGGCCGCCTGCTGGTGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGc                              >  1:377157/1‑128 (MQ=255)
                                                                                                                                               ccaccaGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAgg    >  6:381826/1‑149 (MQ=255)
                                                                                                                                                caccaGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGt   >  4:284562/1‑149 (MQ=255)
                                                                                                                                                 accaGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTc  >  1:120363/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CGCGTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCG‑CTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGTC  >  NC_002947/1469663‑1469956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑