Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,551,282 G→T 11.7% intergenic (+41/+85) groL → / ← pykA chaperonin GroEL/pyruvate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,551,2820GT11.7% 147.1 / 8.6 51intergenic (+41/+85)groL/pykAchaperonin GroEL/pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base G (27/18);  new base T (2/4);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GGTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGG  >  NC_002947/1551134‑1551425
                                                                                                                                                    |                                                                                                                                               
ggTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTcg                                                                                                                                                 <  7:20015/149‑1 (MQ=255)
              caccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCt                                                                                                                                   >  2:10683/1‑149 (MQ=255)
                 cGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttt                                                                                                                                >  8:336642/1‑149 (MQ=255)
                      ccATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTGCTCGCGAACCGGGGCTTTTTGAtg                                                                                                                           <  5:219818/149‑2 (MQ=255)
                        aTGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGc                                                                                                                         >  5:40220/1‑149 (MQ=255)
                          ggTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTcgc                                                                                                                                                >  4:346557/1‑124 (MQ=255)
                          ggTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTcgc                                                                                                                                                <  3:346557/124‑1 (MQ=255)
                               ccGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCggg                                                                                                                  <  8:29787/149‑1 (MQ=255)
                                 gACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc                                                                                                                <  6:40220/149‑1 (MQ=255)
                                   tcTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGGGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc                                                                                                                <  7:264790/146‑1 (MQ=255)
                                   ccTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCct                                                                                                              <  7:336642/149‑1 (MQ=255)
                                    cTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGATGTCGGGGCctc                                                                                                             >  3:72856/1‑149 (MQ=255)
                                     tGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc                                                                                                                >  8:264790/1‑145 (MQ=255)
                                                aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgggg                                                                                                                                     >  5:177136/1‑113 (MQ=255)
                                                aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgggg                                                                                                                                     <  6:177136/113‑1 (MQ=255)
                                                aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGcac                                                                                                   >  5:391509/1‑147 (MQ=255)
                                                aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTACGCAGcac                                                                                                   >  7:308431/1‑147 (MQ=255)
                                                 aGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGCGCTTTTTTATTGCTGTCGGGGCATCTTCGCAGCACAAg                                                                                                >  4:230875/1‑149 (MQ=255)
                                                   ccAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGc                                                                                              >  4:146872/1‑149 (MQ=255)
                                                         gctgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCgggg                                                                                                                 <  5:165886/124‑1 (MQ=255)
                                                         gctgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCgggg                                                                                                                 >  6:165886/1‑124 (MQ=255)
                                                           tgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCtt                                                                                       >  3:18784/1‑148 (MQ=255)
                                                                  ccTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtg                                                                               >  5:339921/1‑149 (MQ=255)
                                                                  ccTGATATGGGGGGCAGGGGGGGCTTGGGCGGCTTTATGTAAGCCAGCATTCCCCCCCTGCACCTTCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTCTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCtt                                                                                <  6:208961/148‑1 (MQ=255)
                                                                   cTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgt                                                                              <  1:10683/149‑1 (MQ=255)
                                                                    tGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCga                                                                                                                                              >  7:281038/1‑84 (MQ=255)
                                                                    tGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCga                                                                                                                                              <  8:281038/84‑1 (MQ=255)
                                                                      atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgtg                                                                             <  5:203490/147‑1 (MQ=255)
                                                                      atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgtg                                                                             <  6:391509/147‑1 (MQ=255)
                                                                        atgggtggcatgggtggcATGGGCGGGATTATGTAAGCCAGCCTTACCCCCCTGCACCATCAAATAGCCGCGGTTCGAGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtg                                                                          <  7:243507/148‑1 (MQ=255)
                                                                           ggtggcatgggtggcatgGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGa                                                                       >  6:86894/1‑148 (MQ=255)
                                                                            gtggcatgggtggcatggGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGaaa                                                                     <  6:339921/149‑1 (MQ=255)
                                                                                catgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg                                                                 <  5:86894/149‑1 (MQ=255)
                                                                                 atgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGt                                                                >  2:104355/1‑149 (MQ=255)
                                                                                  tgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGaagaggccc                                                               >  5:286459/1‑142 (MQ=255)
                                                                                  tgggggtcatgggCGGCTTGATCTAAGCCAGCCTTACCCCCCTCCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTg                                                               <  8:308431/149‑1 (MQ=255)
                                                                                       ggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATc                                                           >  3:245790/1‑148 (MQ=255)
                                                                                                  cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCg                                               >  3:312314/1‑149 (MQ=255)
                                                                                                       tgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGCGGCCTCTTCGCAGCACAAGGATGCGCTTGATCCTTGTGATGTGAAACGAGTGGATCAACCTACCACCGCATc                                           >  3:245442/1‑148 (MQ=255)
                                                                                                             ccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACg                                    >  5:366264/1‑149 (MQ=255)
                                                                                                                gccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa                                                                                     <  8:297602/97‑1 (MQ=255)
                                                                                                                gccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa                                                                                     >  7:297602/1‑97 (MQ=255)
                                                                                                                             gCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAgg                                                                                               >  3:366001/1‑74 (MQ=255)
                                                                                                                             gCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAgg                                                                                               <  4:366001/74‑1 (MQ=255)
                                                                                                                              cACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGc                    >  2:135368/1‑148 (MQ=255)
                                                                                                                                   taaaaacGCCCGGTTTCGCCACAACGGGGGCTTTTTTATTGAGGTCGGGCCCTCTCCGCAGCAAATGGCTGGGTTTTAGCCTGGTGCTGTTAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTgg              <  1:104355/147‑1 (MQ=255)
                                                                                                                                     aaaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTg            >  2:163120/1‑149 (MQ=255)
                                                                                                                                       aaaGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCAt                      >  2:83879/1‑137 (MQ=255)
                                                                                                                                       aaaGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCAt                      <  1:83879/137‑1 (MQ=255)
                                                                                                                                           ccccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTg               >  3:4475/1‑140 (MQ=255)
                                                                                                                                           ccccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTg               <  4:4475/140‑1 (MQ=255)
                                                                                                                                           ccccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCAcc       >  6:265533/1‑148 (MQ=255)
                                                                                                                                             ccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt    >  7:151913/1‑149 (MQ=255)
                                                                                                                                               ggTTCTCGAGAACCGGGGCTTTATTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATgg  <  8:317720/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGG  >  NC_002947/1551134‑1551425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑