Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 194,608 A→C 11.5% E38D (GAA→GAC) ‡ PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947194,6080AC11.5% 170.1 / 13.6 61E38D (GAA→GAC) ‡PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base C (3/4);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

AGGGAAGTACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGT  >  NC_002947/194460‑194754
                                                                                                                                                    |                                                                                                                                                  
aGGGAAGTACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGaa                                                                                                                                                    <  7:115260/149‑1 (MQ=255)
       tACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCa                                                                                                                                             <  7:203677/149‑1 (MQ=255)
         ccccATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCAc                                                                                                                                            <  6:252582/148‑1 (MQ=255)
          cccATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCg                                                                                                                                          >  7:62113/1‑149 (MQ=255)
            cATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGc                                                                                                                                                <  3:302638/141‑1 (MQ=255)
            cATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGc                                                                                                                                                >  4:302638/1‑141 (MQ=255)
            cATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGACCGGTGAGCACTTCCTCACCggc                                                                                                                                        <  8:187660/149‑2 (MQ=255)
             aTCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCggcc                                                                                                                                       <  3:216065/149‑3 (MQ=255)
             aTCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGACCGGTGAGCACTTCCTCACCggcc                                                                                                                                       <  4:33049/149‑3 (MQ=255)
                 ggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCTGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCg                                                                                                                                          <  6:269668/142‑1 (MQ=255)
                 ggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCAGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCg                                                                                                                                          >  5:269668/1‑142 (MQ=255)
                       acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCgg                                                                                                                                         >  2:271800/1‑137 (MQ=255)
                       acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCgg                                                                                                                                         <  1:271800/137‑1 (MQ=255)
                                 cAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                    <  8:2069/148‑1 (MQ=255)
                                  aaTGAGCAGCGTTGTATCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGt                                                                                                                                  <  3:215707/133‑1 (MQ=255)
                                  aaTGAGCAGCGTTGTATCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGt                                                                                                                                  >  4:215707/1‑133 (MQ=255)
                                  aaTGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTgg                                                                                                                  <  5:222954/149‑1 (MQ=255)
                                        cagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctgg                                                                                                            >  4:243216/1‑149 (MQ=255)
                                         agcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctggc                                                                                                           >  8:300514/1‑149 (MQ=255)
                                         agcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctggc                                                                                                           >  1:278226/1‑149 (MQ=255)
                                         agcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctggc                                                                                                           >  4:82929/1‑149 (MQ=255)
                                               gTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                    <  4:188070/134‑1 (MQ=255)
                                               gTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                    >  3:188070/1‑134 (MQ=255)
                                                tAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGc                                                                                                    <  4:124125/149‑1 (MQ=255)
                                                 aGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGcc                                                                                                   >  7:353836/1‑149 (MQ=255)
                                                        gTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGc                                                                                             <  8:350249/148‑1 (MQ=255)
                                                         tCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCt                                                                                            >  6:30674/1‑148 (MQ=255)
                                                         tCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCt                                                                                            <  5:30674/148‑1 (MQ=255)
                                                         tCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTc                                                                                           >  8:98269/1‑149 (MQ=255)
                                                           aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACt                                                                                                                      <  2:15663/120‑1 (MQ=255)
                                                           aaaaGCATTGTCGGCCAGGTTATTGCGATATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACt                                                                                                                      >  1:15663/1‑120 (MQ=255)
                                                             aaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGGCCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGCTGGCGCAGTTACTTTGGAGCTGGCTGATGGACGACTGCTCCATc                                                                                       >  6:252735/1‑149 (MQ=255)
                                                                    gTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACg                                                                                >  6:160787/1‑149 (MQ=255)
                                                                              ttATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCaa                                                                      <  8:62113/149‑1 (MQ=255)
                                                                                   gCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                 >  6:115174/1‑149 (MQ=255)
                                                                                           cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                         >  3:163737/1‑149 (MQ=255)
                                                                                            ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATgc                                                        >  5:24494/1‑149 (MQ=255)
                                                                                             cAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGTTGGCGCAGTTACTTTGGAgctgg                                                                                                            >  5:272324/1‑96 (MQ=255)
                                                                                             cAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctgg                                                                                                            <  6:272324/96‑1 (MQ=255)
                                                                                                  ggCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGa                                                  <  6:24494/149‑1 (MQ=255)
                                                                                                     aTCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAg                                               >  1:7025/1‑149 (MQ=255)
                                                                                                     aTCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAg                                               >  8:169989/1‑149 (MQ=255)
                                                                                                        cGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAg                                            <  3:243216/149‑1 (MQ=255)
                                                                                                                     tCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAg                                <  7:169989/148‑1 (MQ=255)
                                                                                                                      cGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                  >  4:76821/1‑97 (MQ=255)
                                                                                                                      cGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                  <  3:76821/97‑1 (MQ=255)
                                                                                                                        aaGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAg                            <  7:300514/149‑1 (MQ=255)
                                                                                                                          ggTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGAc                                     >  3:227785/1‑138 (MQ=255)
                                                                                                                          ggTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGAc                                     <  4:227785/138‑1 (MQ=255)
                                                                                                                           gTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcg                         <  5:115174/149‑1 (MQ=255)
                                                                                                                           gTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcg                         <  5:160787/149‑1 (MQ=255)
                                                                                                                             gACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcgg                        >  8:29195/1‑148 (MQ=255)
                                                                                                                              aCCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcgg                        >  6:94272/1‑147 (MQ=255)
                                                                                                                                cGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGc                    >  1:213013/1‑149 (MQ=255)
                                                                                                                                      ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTa                                             >  6:192946/1‑118 (MQ=255)
                                                                                                                                      ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTa                                             <  5:192946/118‑1 (MQ=255)
                                                                                                                                      ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGc              <  4:163737/149‑1 (MQ=255)
                                                                                                                                       ggaccGGTGAGCACTTCCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGcc             >  7:120480/4‑149 (MQ=255)
                                                                                                                                          ccGGTGAGCACTTCCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCcgcg          >  8:337003/1‑149 (MQ=255)
                                                                                                                                            ggTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGcc        <  8:353836/149‑1 (MQ=255)
                                                                                                                                              tGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCtt      <  2:278226/149‑1 (MQ=255)
                                                                                                                                                  gAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGt  <  5:94272/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGGAAGTACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGT  >  NC_002947/194460‑194754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑