Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,575,200	T→A	6.6%	intergenic (‑186/+24)	pcaP ← / ← galP‑II	porin/porin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,575,200	0	T	A	6.6%	271.1 / 6.9	91	intergenic (‑186/+24)	pcaP/galP‑II	porin/porin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (41/44);  new base A (2/4);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

CTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGC  >  NC_002947/1575052‑1575347                                                                                                                                                     |                                                                                                                                                    cTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGtt                                                                                                                                                     >  6:40907/1‑149 (MQ=255)  tGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGtt                                                                                                                                                     >  6:51214/1‑148 (MQ=255)  tGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGtt                                                                                                                                                     >  6:56805/1‑148 (MQ=255)    aTAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTAt                                                                                                                                                  >  8:162964/1‑149 (MQ=255)    aTAGTGATAAGGATAGCCTTCATTCACCACAATCATCATCTCAGCGAATTGCGTCAGCTCGCACTTGGCAATTCATGGCAGGGTTTTCATCAAGCTGTTGCAAATGATGCCTGCAAAAGGGAGGGAGTCTTACCTATTCTCGGGTTTa                                                                                                                                                   >  5:188257/1‑148 (MQ=255)        tGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCcgc                                                                                                                                              <  1:249210/149‑1 (MQ=255)          aTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCcgcg                                                                                                                                             >  5:1438/1‑148 (MQ=255)          aTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCcgcg                                                                                                                                             >  2:322865/1‑148 (MQ=255)          aTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATAcgcg                                                                                                                                             >  7:308191/1‑148 (MQ=255)            cAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCcgcg                                                                                                                                             >  2:331857/1‑146 (MQ=255)             aGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAag                                                                                                                                          <  8:144460/148‑1 (MQ=255)              ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                        <  4:349859/149‑1 (MQ=255)              ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAAATCATCGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAagag                                                                                                                                        >  6:83749/1‑149 (MQ=255)                aTAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                      <  2:10363/149‑1 (MQ=255)                   gCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCaa                                                                                                                                   <  5:40907/149‑1 (MQ=255)                    ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAt                                                                                                                                  >  6:9768/1‑149 (MQ=255)                     cTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTATCATCACGCTGTTGCCAATGATGCCTTCAAAAGGCAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCaaat                                                                                                                                 >  1:337321/1‑147 (MQ=255)                      tGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                                 >  6:167807/1‑148 (MQ=255)                        cAGTTACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAg                                                                                                                              <  4:331090/149‑1 (MQ=255)                        cAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAg                                                                                                                              <  5:9768/149‑1 (MQ=255)                           tCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               <  6:152016/145‑1 (MQ=255)                           tCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               >  5:152016/1‑145 (MQ=255)                           tCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTca                                                                                                                           >  4:164650/1‑149 (MQ=255)                           tCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTca                                                                                                                           <  7:162964/149‑1 (MQ=255)                           tCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTca                                                                                                                           >  2:343517/1‑149 (MQ=255)                             accaccATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               >  1:194673/1‑143 (MQ=255)                             accaccATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               <  2:194673/143‑1 (MQ=255)                               caccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACag                                                                                                                        >  2:332439/1‑148 (MQ=255)                                 ccaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGATGAGGCCAATTGAGTCACagaag                                                                                                                     <  7:239042/149‑1 (MQ=255)                                  catcatCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaaga                                                                                                                    <  7:271901/149‑1 (MQ=255)                                  catcatCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaaga                                                                                                                    <  2:109270/149‑1 (MQ=255)                                  aatcatcAGCTCAGCGAATGGCGGAAGCTCGCACTGGGCAATTCATGGAAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGTGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGTCCAATTGAGTCACagaaga                                                                                                                    <  7:223647/148‑1 (MQ=255)                                      atcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGaata                                                                                                                                         <  6:350427/124‑3 (MQ=255)                                      atcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCAAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGaata                                                                                                                                         >  5:350427/1‑122 (MQ=255)                                        cagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                      >  8:74959/1‑125 (MQ=255)                                        cagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                      <  7:74959/125‑1 (MQ=255)                                        cagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTcac                                                                                                                          >  6:215194/1‑137 (MQ=255)                                        cagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTcac                                                                                                                          <  5:215194/137‑1 (MQ=255)                                           ctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGa                                                                                                           >  4:85444/1‑149 (MQ=255)                                            tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGa                                                                                                           >  2:363025/1‑148 (MQ=255)                                               gcGAATGGCGGCAGCTCGCACTTGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAAc                                                                                                       <  5:51214/149‑1 (MQ=255)                                               gcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAAc                                                                                                       <  5:56805/149‑1 (MQ=255)                                                  aaTGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTca                                                                                                    <  6:1438/149‑1 (MQ=255)                                                      gcggcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               >  6:78195/1‑118 (MQ=255)                                                      gcggcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               <  5:78195/118‑1 (MQ=255)                                                       cggcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATc                                                                                               <  3:169399/149‑1 (MQ=255)                                                         gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAgg                                                                                                                                       <  8:306635/107‑1 (MQ=38)                                                         gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCaa                                                                                             <  1:343517/149‑1 (MQ=255)                                                              tCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGt                                                                                        >  6:347030/1‑149 (MQ=255)                                                              tCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGt                                                                                        >  8:174903/1‑149 (MQ=255)                                                                gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtc                                                                                      >  6:91407/1‑149 (MQ=255)                                                                gCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtc                                                                                      >  5:327690/1‑149 (MQ=255)                                                                 cACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtct                                                                                     >  8:168832/1‑149 (MQ=255)                                                                   cTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCa                                                                                   <  1:363025/149‑1 (MQ=255)                                                                    tGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCAt                                                                                  <  3:85444/149‑1 (MQ=255)                                                                      ggCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCAt                                                                                  <  7:174903/147‑1 (MQ=255)                                                                      ggCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATc                                                                                 <  1:331857/148‑1 (MQ=255)                                                                              aTGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGtt                                                                        >  1:50399/1‑149 (MQ=255)                                                                              aTGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGtc                                                                        <  3:164650/149‑2 (MQ=255)                                                                                ggCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGtt                                                                                       >  6:389597/1‑132 (MQ=255)                                                                                ggCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGtt                                                                                       <  5:389597/132‑1 (MQ=255)                                                                                ggCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGtt                                                                                       >  6:124897/1‑132 (MQ=255)                                                                                ggCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGtt                                                                                       <  5:124897/132‑1 (MQ=255)                                                                                 gCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGt                                                                     >  5:154345/1‑149 (MQ=255)                                                                                  cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               >  2:113585/1‑90 (MQ=255)                                                                                  cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                               <  1:113585/90‑1 (MQ=255)                                                                                       ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                               >  3:367226/1‑149 (MQ=255)                                                                                       ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                               <  2:50399/149‑1 (MQ=255)                                                                                       ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGaa                                                               <  7:168832/149‑1 (MQ=255)                                                                                         ttCATACCGCTTTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAg                                                              <  8:308191/148‑1 (MQ=255)                                                                                           catcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGt                                                                                                                             >  5:380041/1‑83 (MQ=255)                                                                                           catcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGt                                                                                                                             <  6:380041/83‑1 (MQ=255)                                                                                            atcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTcgc                                                          >  7:94409/1‑149 (MQ=255)                                                                                                 gCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACg                                                      >  7:349949/1‑148 (MQ=255)                                                                                                     ttGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCAt                                                   <  8:349949/147‑1 (MQ=255)                                                                                                              atgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                        <  5:347030/149‑1 (MQ=255)                                                                                                               tgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                        <  5:91407/148‑1 (MQ=255)                                                                                                                gCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgcgc                                      >  8:191448/1‑149 (MQ=255)                                                                                                                gCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgcgc                                      >  2:313140/1‑149 (MQ=255)                                                                                                                       aaaGGGAGGGCGTCTGGCCTATTCGGGGGTTTATCCGCGAAGAGGCCAATTGAGGCACTGTATATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACTGGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCa                               <  2:337321/149‑1 (MQ=255)                                                                                                                       aaaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCa                               <  1:322865/149‑1 (MQ=255)                                                                                                                       aaaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCa                               <  5:167807/149‑1 (MQ=255)                                                                                                                        aaGGGAGGGCGGCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAg                              <  5:83749/149‑1 (MQ=255)                                                                                                                         agggagggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                             <  6:154345/149‑1 (MQ=255)                                                                                                                             agggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                   >  2:334062/1‑139 (MQ=255)                                                                                                                             agggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                   <  1:334062/139‑1 (MQ=255)                                                                                                                                         ctcttCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                             >  2:140774/4‑133 (MQ=255)                                                                                                                                         ctcttCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                             <  1:140774/130‑1 (MQ=255)                                                                                                                                         cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGc             >  3:360606/1‑149 (MQ=255)                                                                                                                                          tATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTg                 <  5:8028/144‑1 (MQ=255)                                                                                                                                          tATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTg                 >  6:8028/1‑144 (MQ=255)                                                                                                                                             tCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGGAGTCGCGACGCATGGTCGAGTTGCGCCATTTc                                >  3:126767/1‑126 (MQ=255)                                                                                                                                             tCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGGAGTCGCGACGCATGGTCGAGTTGCGCCATTTc                                <  4:126767/126‑1 (MQ=255)                                                                                                                                              cgcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc        >  5:39925/1‑149 (MQ=255)                                                                                                                                                    tttATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGc  <  6:39925/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CTGATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACTGC  >  NC_002947/1575052‑1575347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑