Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 194,609 G→T 11.5% V39L (GTG→TTG) ‡ PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947194,6090GT11.5% 173.7 / 11.8 61V39L (GTG→TTG) ‡PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base T (3/4);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

TACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTGG  >  NC_002947/194467‑194756
                                                                                                                                              |                                                                                                                                                   
tACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCa                                                                                                                                               <  7:203677/149‑1 (MQ=255)
  ccccATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCAc                                                                                                                                              <  6:252582/148‑1 (MQ=255)
   cccATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCg                                                                                                                                            >  7:62113/1‑149 (MQ=255)
     cATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGc                                                                                                                                                  <  3:302638/141‑1 (MQ=255)
     cATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGc                                                                                                                                                  >  4:302638/1‑141 (MQ=255)
     cATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGACCGGTGAGCACTTCCTCACCggc                                                                                                                                          <  8:187660/149‑2 (MQ=255)
      aTCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCggcc                                                                                                                                         <  3:216065/149‑3 (MQ=255)
      aTCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGACCGGTGAGCACTTCCTCACCggcc                                                                                                                                         <  4:33049/149‑3 (MQ=255)
          ggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCTGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCg                                                                                                                                            <  6:269668/142‑1 (MQ=255)
          ggTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCAGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCg                                                                                                                                            >  5:269668/1‑142 (MQ=255)
                acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCgg                                                                                                                                           >  2:271800/1‑137 (MQ=255)
                acGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGCACTTCCTCACCgg                                                                                                                                           <  1:271800/137‑1 (MQ=255)
                          cAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                      <  8:2069/148‑1 (MQ=255)
                           aaTGAGCAGCGTTGTATCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGt                                                                                                                                    >  4:215707/1‑133 (MQ=255)
                           aaTGAGCAGCGTTGTATCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGt                                                                                                                                    <  3:215707/133‑1 (MQ=255)
                           aaTGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTgg                                                                                                                    <  5:222954/149‑1 (MQ=255)
                                 cagcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctgg                                                                                                              >  4:243216/1‑149 (MQ=255)
                                  agcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctggc                                                                                                             >  4:82929/1‑149 (MQ=255)
                                  agcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctggc                                                                                                             >  8:300514/1‑149 (MQ=255)
                                  agcGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctggc                                                                                                             >  1:278226/1‑149 (MQ=255)
                                        gTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                      >  3:188070/1‑134 (MQ=255)
                                        gTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACttt                                                                                                                      <  4:188070/134‑1 (MQ=255)
                                         tAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGc                                                                                                      <  4:124125/149‑1 (MQ=255)
                                          aGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGcc                                                                                                     >  7:353836/1‑149 (MQ=255)
                                                 gTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGc                                                                                               <  8:350249/148‑1 (MQ=255)
                                                  tCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCt                                                                                              <  5:30674/148‑1 (MQ=255)
                                                  tCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCt                                                                                              >  6:30674/1‑148 (MQ=255)
                                                  tCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTc                                                                                             >  8:98269/1‑149 (MQ=255)
                                                    aaaaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACt                                                                                                                        <  2:15663/120‑1 (MQ=255)
                                                    aaaaGCATTGTCGGCCAGGTTATTGCGATATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACt                                                                                                                        >  1:15663/1‑120 (MQ=255)
                                                      aaGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGGCCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGCTGGCGCAGTTACTTTGGAGCTGGCTGATGGACGACTGCTCCATc                                                                                         >  6:252735/1‑149 (MQ=255)
                                                             gTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACg                                                                                  >  6:160787/1‑149 (MQ=255)
                                                                       ttATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCaa                                                                        <  8:62113/149‑1 (MQ=255)
                                                                            gCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAg                                                                   >  6:115174/1‑149 (MQ=255)
                                                                                    cccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATg                                                           >  3:163737/1‑149 (MQ=255)
                                                                                     ccAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATgc                                                          >  5:24494/1‑149 (MQ=255)
                                                                                      cAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGTTGGCGCAGTTACTTTGGAgctgg                                                                                                              >  5:272324/1‑96 (MQ=255)
                                                                                      cAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAgctgg                                                                                                              <  6:272324/96‑1 (MQ=255)
                                                                                           ggCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGa                                                    <  6:24494/149‑1 (MQ=255)
                                                                                              aTCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAg                                                 >  1:7025/1‑149 (MQ=255)
                                                                                              aTCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAg                                                 >  8:169989/1‑149 (MQ=255)
                                                                                                 cGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAg                                              <  3:243216/149‑1 (MQ=255)
                                                                                                              tCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAg                                  <  7:169989/148‑1 (MQ=255)
                                                                                                               cGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                    <  3:76821/97‑1 (MQ=255)
                                                                                                               cGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGa                                                                                    >  4:76821/1‑97 (MQ=255)
                                                                                                                 aaGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAg                              <  7:300514/149‑1 (MQ=255)
                                                                                                                   ggTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGAc                                       <  4:227785/138‑1 (MQ=255)
                                                                                                                   ggTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGAc                                       >  3:227785/1‑138 (MQ=255)
                                                                                                                    gTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcg                           <  5:115174/149‑1 (MQ=255)
                                                                                                                    gTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcg                           <  5:160787/149‑1 (MQ=255)
                                                                                                                      gACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcgg                          >  8:29195/1‑148 (MQ=255)
                                                                                                                       aCCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAggcgg                          >  6:94272/1‑147 (MQ=255)
                                                                                                                         cGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGc                      >  1:213013/1‑149 (MQ=255)
                                                                                                                               ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTa                                               >  6:192946/1‑118 (MQ=255)
                                                                                                                               ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTa                                               <  5:192946/118‑1 (MQ=255)
                                                                                                                               ttGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGc                <  4:163737/149‑1 (MQ=255)
                                                                                                                                ggaccGGTGAGCACTTCCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGcc               >  7:120480/4‑149 (MQ=255)
                                                                                                                                   ccGGTGAGCACTTCCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCcgcg            >  8:337003/1‑149 (MQ=255)
                                                                                                                                     ggTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGcc          <  8:353836/149‑1 (MQ=255)
                                                                                                                                       tGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCtt        <  2:278226/149‑1 (MQ=255)
                                                                                                                                           gAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGt    <  5:94272/149‑1 (MQ=255)
                                                                                                                                              gTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCt                                     <  2:75223/113‑1 (MQ=255)
                                                                                                                                              gTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCt                                     >  1:75223/1‑113 (MQ=255)
                                                                                                                                              gTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTgg  >  2:164851/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TACCCCATCGGGTCACACGGAGAGTCCAATGAGCAGCGTTGTAGCCATCGTCAAAAGCATTGTCGGCCAGGTTATTGCGGTATCCCCAGAGGGCATCCGGCGTGTACTTATCGAAGGTGACCGCCTGTTGGCCGGTGAGGAAGTGCTCACCGGTCCGGGTGGCGCAGTTACTTTGGAGCTGGCTGATGGCCGACTGCTCGATCTTGGACGTGACAGCCAATGGAGCGCCGATGCGCCTGACAGTAGCACCGACCTGAGCCAGGCGGCAGCCCAGGCCGCGCCTTCGGTGG  >  NC_002947/194467‑194756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑