Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,581,266 G→T 100% A44D (GCC→GAC)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,581,2660GT95.8% 144.9 / ‑1.2 48A44D (GCC→GAC) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (20/25);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATG  >  NC_002947/1581126‑1581397
                                                                                                                                            |                                                                                                                                   
tAATGCTCATGCTGCCGTCGGAGTTACTTTCCGACGACACAAAACGCAGGTTGTCGATACCGTTGAGCTGCTGCACGAACACCTGCACCACGGTGTCCAGCACGGTTTGCGCCGAGGCGCCCGCCTAGGTCACGgccaagtcgatgc                                                                                                                               >  4:261280/1‑146 (MQ=255)
     gTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTggcg                                                                                                                         <  1:332079/148‑1 (MQ=255)
        aTGCTGCCGTCGGAGTTACTTTCCGACGAGACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGgcgc                                                                                                                     >  5:300717/1‑149 (MQ=255)
          gctgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGa                                                                                                                   >  3:178578/1‑149 (MQ=255)
            tgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATa                                                                                                                 >  6:193119/1‑149 (MQ=255)
               cGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                              <  4:165394/149‑1 (MQ=255)
               cGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                              <  5:44760/149‑1 (MQ=255)
                    gAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTa                                                                                                         >  7:193788/1‑149 (MQ=255)
                    gAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTa                                                                                                         <  4:178578/149‑1 (MQ=255)
                         aCTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTgg                                                                                                     <  5:81242/148‑1 (MQ=255)
                             tCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGAt                                                                                                >  5:52652/1‑149 (MQ=255)
                               cgacgaCACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCg                                                                                              <  3:144556/149‑1 (MQ=255)
                               cgacgaCACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGAAACTGCGGTACTCGTTGAGCg                                                                                              >  4:210682/1‑149 (MQ=255)
                                gacgacGCATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCAGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGACGATGGCTGGCCGCGCGATAc                                                                                                                >  4:375130/1‑130 (MQ=255)
                                  cgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCa                                                                                           <  6:52652/149‑1 (MQ=255)
                                      acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGgcgatgtcgatg                                                                                                                                >  2:95746/1‑108 (MQ=255)
                                      acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGgcgatgtcgatg                                                                                                                                <  1:95746/108‑1 (MQ=255)
                                        aTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCCCCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCtt                                                                                       >  6:178014/1‑147 (MQ=255)
                                         tAACGCAGGGTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAg                                                                                    <  7:137444/149‑1 (MQ=255)
                                           aCGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGa                                                                                  >  2:246319/1‑149 (MQ=255)
                                             gCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATg                                                                                <  2:336380/149‑1 (MQ=255)
                                               aGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACAACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGa                                                                              >  2:185659/1‑149 (MQ=255)
                                                     tCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGc                                                                        <  6:300717/149‑1 (MQ=255)
                                                             gTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAc                                                                  >  2:17974/1‑147 (MQ=255)
                                                                     gctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGAt                                                         >  7:36058/1‑148 (MQ=255)
                                                                           cGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATc                                                        <  6:237049/143‑1 (MQ=255)
                                                                           cGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATc                                                        >  5:237049/1‑143 (MQ=255)
                                                                            gATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCGGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGc                                                                                            >  6:113029/1‑106 (MQ=255)
                                                                            gATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGTGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAg                                                  <  3:210682/148‑1 (MQ=255)
                                                                            gATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGc                                                                                            <  5:113029/106‑1 (MQ=255)
                                                                              tCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCg                                               <  1:185659/149‑1 (MQ=255)
                                                                              tCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCg                                               <  1:246319/149‑1 (MQ=255)
                                                                                aCCTGCACCACGGTGTCCTTCACGGTTTGCGCCGAGGCGCGCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGa                                              <  3:261280/148‑1 (MQ=255)
                                                                                   tGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCAc                                          >  3:366667/1‑149 (MQ=255)
                                                                                       ccacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                              <  5:75432/77‑1 (MQ=255)
                                                                                       ccacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                              >  6:75432/1‑77 (MQ=255)
                                                                                        cacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTc                                                                                      <  1:227992/100‑1 (MQ=255)
                                                                                        cacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTc                                                                                      >  2:227992/1‑100 (MQ=255)
                                                                                          cGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGc                                    >  8:222751/1‑148 (MQ=255)
                                                                                          cGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGc                                    >  3:90576/1‑148 (MQ=255)
                                                                                            gtgtCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGaa                                 <  4:90576/149‑1 (MQ=255)
                                                                                              gtCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGa                                  >  4:54569/1‑146 (MQ=255)
                                                                                              gtCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGa                                  <  3:54569/146‑1 (MQ=255)
                                                                                                 cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGc                                                                                            <  3:30352/85‑1 (MQ=255)
                                                                                                 cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGc                                                                                            >  4:30352/1‑85 (MQ=255)
                                                                                                 cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCg                            <  7:311651/149‑1 (MQ=255)
                                                                                                    cACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCggg                          <  1:17974/148‑1 (MQ=255)
                                                                                                     aCGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGc                         >  4:348722/1‑148 (MQ=255)
                                                                                                       ggTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGcgat                      <  8:193788/149‑1 (MQ=255)
                                                                                                           tGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGcgatcga                   <  5:178014/148‑1 (MQ=255)
                                                                                                                           ggTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGTGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATg  <  5:193119/149‑1 (MQ=255)
                                                                                                                                     ggcgatgtcgatGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGaa            <  6:254136/129‑1 (MQ=255)
                                                                                                                                     ggcgatgtcgatGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGaa            >  5:254136/1‑129 (MQ=255)
                                                                                                                                            |                                                                                                                                   
TAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATG  >  NC_002947/1581126‑1581397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑