Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,583,319 T→G 8.2% Y170D (TAT→GAT)  ttgR → HTH‑type transcriptional regulator TtgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,583,3190TG 8.2% 164.3 / 3.2 57Y170D (TAT→GAT) ttgRHTH‑type transcriptional regulator TtgR
Reads supporting (aligned to +/- strand):  ref base T (30/22);  new base G (3/2);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACATGTGTGAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGATTGTGAGG  >  NC_002947/1583174‑1583466
                                                                                                                                                 |                                                                                                                                                   
tGACATGTGTGAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATg                                                                                                                                                  >  6:315545/1‑149 (MQ=255)
        gtgAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATGACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGGGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCGATGTCGATGGc                                                                                                                                          >  3:289599/1‑149 (MQ=255)
        gtgAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGc                                                                                                                                          >  5:249303/1‑149 (MQ=255)
        gtgAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCGATGTCGATGGc                                                                                                                                          >  3:294629/1‑149 (MQ=255)
        gtgAAATTCGCCAGCAGCGCCAGAGTCCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATTGc                                                                                                                                          >  2:320122/1‑149 (MQ=255)
         tgAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGACCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGc                                                                                                                                          <  2:219075/148‑1 (MQ=255)
         tgAAATTCGCCAGCAGCGCCAGAGTGCGGTGATGGATTGCGACAAGGGCATCACCCTGGCGCTGGCCAATGCAGTAGGCCGTGGGCAGTTGGCAGGCGAGCGGCATGTCGAGCGCGCGGCGGTGGCCATGTTAGCGTATGTCGATGGcc                                                                                                                                         >  6:99697/1‑149 (MQ=255)
                 gCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCgg                                                                                                                                  <  8:288604/148‑1 (MQ=255)
                       agcGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTgg                                                                                                                           <  7:197827/149‑1 (MQ=255)
                          gcCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTa                                                                                                                        >  8:149940/1‑149 (MQ=255)
                                 tGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATtgctgc                                                                                                                 <  4:111223/149‑1 (MQ=255)
                                      tGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGAc                                                                                                            <  5:315545/149‑1 (MQ=255)
                                           gctgcccACACGGGCAGCACCCTGCCGCTGGCCCATGCCGCCCGCCGGGGCCCGTTGCCTGGCGAGCCGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATCTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAgtgt                                                                                                       <  4:305509/144‑1 (MQ=255)
                                             ttGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTc                                                                                                      >  4:99805/1‑148 (MQ=255)
                                                ccACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGACTGACAGTGTCGATc                                                                                                  <  2:378134/149‑1 (MQ=255)
                                                ccACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGACTGACAGTGTCGATc                                                                                                  >  1:378134/1‑149 (MQ=255)
                                                ccACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGACTGATCGGGCGCTTGCTATTGCTGCCTCACAGTGTCGATc                                                                                                  >  3:43196/1‑149 (MQ=255)
                                                 cacaAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCt                                                                                                 >  5:329147/1‑149 (MQ=255)
                                                 cacaAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGGCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGCCAGTGTCGATCt                                                                                                 >  8:327767/1‑149 (MQ=255)
                                                  acaAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTGGCCGATGTCGATGGCGTGATCGGGCGGTGGCTATTGATGCCTGACTGTGTCGATCTg                                                                                                >  5:116366/1‑149 (MQ=255)
                                                    aaGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTgg                                                                                                                           >  8:308642/1‑120 (MQ=255)
                                                    aaGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGtt                                                                                              <  1:48067/149‑1 (MQ=255)
                                                    aaGGGAATGACACTGGCGCTTGCCAATGCCGTACGCCGGGGCCAGTTTCCTGGCGAGCTTGATGTCGAGCGCGCGGCGGTGGCCATGTGTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGtt                                                                                              <  5:99697/149‑1 (MQ=255)
                                                      gggCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTgg                                                                                            <  6:249303/149‑1 (MQ=255)
                                                       ggCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGCTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTggg                                                                                           >  4:56938/1‑149 (MQ=255)
                                                         cttCACCCTGGCGCTGGCCAATGCCGGACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGGCGATCTGTTGGGTg                                                                                         <  3:5691/147‑1 (MQ=255)
                                                                    cgcTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGaaaaa                                                                              <  1:55240/149‑1 (MQ=255)
                                                                           cAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTc                                                                        >  3:321398/1‑148 (MQ=255)
                                                                            aaTGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGGGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGa                                                                      <  3:56938/149‑1 (MQ=255)
                                                                            aaTGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCg                                                                                                     >  1:120103/1‑118 (MQ=255)
                                                                            aaTGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCg                                                                                                     <  2:120103/118‑1 (MQ=255)
                                                                                      gCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTg                                                            >  6:271691/1‑149 (MQ=255)
                                                                                              cAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATAtgct                                                    >  5:198799/1‑149 (MQ=255)
                                                                                                gTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATAtgctgc                                                  >  3:303482/1‑149 (MQ=255)
                                                                                                gTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATAtgctgc                                                  <  1:204163/149‑1 (MQ=255)
                                                                                                     cTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGa                                                                      >  1:345650/1‑124 (MQ=255)
                                                                                                     cTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGa                                                                      <  2:345650/124‑1 (MQ=255)
                                                                                                      tGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACa                                                                                                           <  5:112681/86‑1 (MQ=255)
                                                                                                      tGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACa                                                                                                           >  6:112681/1‑86 (MQ=255)
                                                                                                      tGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCtt                                              >  1:43172/1‑147 (MQ=255)
                                                                                                        gCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGc                                          >  1:339225/1‑149 (MQ=255)
                                                                                                            gCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCgg                                      <  4:321398/149‑1 (MQ=255)
                                                                                                             cTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCGATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGc                                     <  4:294629/149‑1 (MQ=255)
                                                                                                                gATGTCTAGCGCGAGTCGGTGGCCATGTTTGCCGATGGCTATGTCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGc                                     <  4:289599/146‑1 (MQ=255)
                                                                                                                    tCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGAGCTTGAGCCCGGCTCTgcg                               >  2:201160/1‑148 (MQ=255)
                                                                                                                        gcgcgcGGGGGTTGGCATGATTTCCTATGTCGATGGCCTGATCTGTCGCGGGGTATTGCTGGTTGTCTTTGTCTATCTGTTGGGTTATGTATAATAATGGGTCTACAGCGGTCTGGATATGCTGTGCTTGAGCCGGTCTCTGCGAAAAt                          <  4:43196/149‑1 (MQ=255)
                                                                                                                          gcgcgGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGa                        >  3:98026/1‑149 (MQ=255)
                                                                                                                          gcgcgGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGa                        >  6:85073/1‑149 (MQ=255)
                                                                                                                                   tGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACAGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGt                >  7:260567/1‑148 (MQ=255)
                                                                                                                                    ggCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGtt               >  7:165320/1‑148 (MQ=255)
                                                                                                                                     gCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTaa             <  1:119315/149‑1 (MQ=255)
                                                                                                                                       cATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAgg           >  6:42369/1‑149 (MQ=255)
                                                                                                                                          gTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGAtt        >  2:124219/1‑149 (MQ=255)
                                                                                                                                           tttGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGAtt        <  1:180148/148‑1 (MQ=255)
                                                                                                                                            ttGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGAGCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGATtgt      >  6:261031/1‑149 (MQ=255)
                                                                                                                                             tGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGATtgt      <  2:339225/148‑1 (MQ=255)
                                                                                                                                                cTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGATTGTGAgg  >  5:237996/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGACATGTGTGAAATTCGCCAGCAGCGCCAGAGTGCGGTGCTGGATTGCCACAAGGGCATCACCCTGGCGCTGGCCAATGCCGTACGCCGGGGCCAGTTGCCTGGCGAGCTGGATGTCGAGCGCGCGGCGGTGGCCATGTTTGCCTATGTCGATGGCCTGATCGGGCGCTGGCTATTGCTGCCTGACAGTGTCGATCTGTTGGGTGATGTCGAAAAATGGGTCGACACCGGGCTGGATATGCTGCGCTTGAGCCCGGCTCTGCGCAAATGACACTTTGTTAAGGATTGTGAGG  >  NC_002947/1583174‑1583466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑