Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,597,883 C→A 5.5% Q293H (CAG→CAT)  dctB ← C4‑dicarboxylate transport sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,597,8830CA 5.5% 231.0 / 3.2 72Q293H (CAG→CAT) dctBC4‑dicarboxylate transport sensor protein
Reads supporting (aligned to +/- strand):  ref base C (31/36);  new base A (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

GCTCCTCGACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCGTCAGCACCTGATGGTGTAGCGG  >  NC_002947/1597735‑1598029
                                                                                                                                                    |                                                                                                                                                   
gcTCCTCGACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGAcc                                                                                                                                                     >  3:68383/1‑149 (MQ=255)
gcTCCTCGACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGAcc                                                                                                                                                     >  5:103535/1‑149 (MQ=255)
  tcctcGACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTg                                                                                                                                                   >  6:227127/1‑149 (MQ=255)
     tcGACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCgg                                                                                                                                                >  2:58577/1‑149 (MQ=255)
       gACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGtt                                                                                                                                              <  5:239571/149‑1 (MQ=255)
                ttCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCgcagca                                                                                                                                     <  4:85726/149‑1 (MQ=255)
                gtCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCgcagca                                                                                                                                     <  3:244262/148‑1 (MQ=255)
                    aGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCGGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACATGCGGTTTGCGCAGCAGGTg                                                                                                                                 >  4:350613/1‑149 (MQ=255)
                    aGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCGGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACATGCGGTTTGCGCAGCAGGTg                                                                                                                                 >  4:356295/1‑149 (MQ=255)
                        cTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCCGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCaa                                                                                                                              <  7:360475/148‑1 (MQ=255)
                         ttGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAgg                                                                                                                            >  1:355183/1‑149 (MQ=255)
                         ttGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAgg                                                                                                                            >  4:348940/1‑149 (MQ=255)
                            ccGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTcc                                                                                                                         <  5:113677/149‑1 (MQ=255)
                            ccGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTc                                                                                                                          >  4:51652/1‑148 (MQ=255)
                             cGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCa                                                                                                                        <  8:61650/149‑1 (MQ=255)
                                cTGAGCGGGCTCAGGCGTGCCAGTCTCATGAGTTTGCTGACTAAAAGGGCGGCGAACACCAGGCTCAGCAAGACGGCGGCTGCGCCTAGCGCTGCATTACGGCCGTCGGAAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAg                                                                                                                       <  4:201268/147‑1 (MQ=255)
                                     cTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGc                                                                                                                >  8:49834/1‑149 (MQ=255)
                                      tGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCTGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGcc                                                                                                               >  7:320683/1‑149 (MQ=255)
                                      tGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGcc                                                                                                               >  2:203037/1‑149 (MQ=255)
                                        gcgcAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCtt                                                                                                             <  6:321702/149‑1 (MQ=255)
                                         cgcAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑c                                                                                                           <  7:49834/149‑1 (MQ=255)
                                          gcAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑c                                                                                                           >  2:197752/1‑148 (MQ=255)
                                                gTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGCCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACgg                                                                                                     >  7:290361/1‑148 (MQ=255)
                                                 tGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCa                                                                                                   <  1:58577/149‑1 (MQ=255)
                                                 tGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCa                                                                                                   <  5:378562/149‑1 (MQ=255)
                                                    caggcgcaggcgTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCTTTACGGCCGTCGGCAGCGACCTGCGCTTTGCGCAGCAGGTGCAAGTTCCAGTATTCGCCTT‑CCCACTGCAGGc                                                                                                >  4:220950/1‑149 (MQ=255)
                                                               gTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCTGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAgg                                                                                     <  8:320683/149‑1 (MQ=255)
                                                                   gCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTTCCCAACGGCAGGCTTTCCCACAGGTAc                                                                                  <  1:197752/149‑1 (MQ=255)
                                                                   gCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACt                                                                                 <  7:132710/149‑1 (MQ=255)
                                                                   gCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACt                                                                                 >  4:54178/1‑149 (MQ=255)
                                                                    cTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTc                                                                                <  6:103535/149‑1 (MQ=255)
                                                                    cTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTc                                                                                <  3:348940/149‑1 (MQ=255)
                                                                      gACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCgg                                                                              >  7:111847/1‑149 (MQ=255)
                                                                       aCGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCgg                                                                              >  5:254002/1‑148 (MQ=255)
                                                                       aCGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCg                                                                               <  3:54178/147‑1 (MQ=255)
                                                                       aCGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCg                                                                               <  1:80677/147‑1 (MQ=255)
                                                                        cGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGt                                                                             <  8:111847/148‑1 (MQ=255)
                                                                         gAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGtac                                                                           <  7:21303/149‑1 (MQ=255)
                                                                           aaaGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGtact                                                                          >  8:324229/1‑148 (MQ=255)
                                                                            aaGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGtactac                                                                        <  1:272962/149‑1 (MQ=255)
                                                                             aGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTAcc                                                                       >  4:266861/1‑149 (MQ=255)
                                                                                   gcgAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCggg                                                                 <  5:159052/149‑1 (MQ=255)
                                                                                   gcgAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGAGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCggg                                                                 >  4:222591/1‑149 (MQ=255)
                                                                                     gAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGc                                                               <  8:290361/149‑1 (MQ=255)
                                                                                      aaCACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGcc                                                              <  2:355183/149‑1 (MQ=255)
                                                                                         acCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTg                                                           >  7:14815/1‑149 (MQ=255)
                                                                                             ggCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCtt                                                            <  7:98369/144‑1 (MQ=255)
                                                                                             ggCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCtt                                                            >  8:98369/1‑144 (MQ=255)
                                                                                              gCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCAcgc                                                      >  5:368550/1‑149 (MQ=255)
                                                                                              gCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCAcgc                                                      <  3:51652/149‑1 (MQ=255)
                                                                                                  aGCCGGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACATGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCgg                                                    <  3:356295/147‑1 (MQ=255)
                                                                                                  aGCCGGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACATGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCgg                                                    <  3:350613/147‑1 (MQ=255)
                                                                                                   gCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCggg                                                                 >  2:235886/1‑133 (MQ=255)
                                                                                                   gCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCggg                                                                 <  1:235886/133‑1 (MQ=255)
                                                                                                      aGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAgg                                              <  6:368550/149‑1 (MQ=255)
                                                                                                       gACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAggg                                             <  6:254002/149‑1 (MQ=255)
                                                                                                        aCGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGt                                            <  2:170176/149‑1 (MQ=255)
                                                                                                         cggcggcggCGCCAAGCGCTGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGCTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTAATACCCTCGGGGCCTTGCACGAGGCTAAGGCTg                                           >  1:41919/1‑149 (MQ=255)
                                                                                                          ggcggcggcGCCAAGCGCGGCATTACGTCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCACCAT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCAAAGGGtcc                                          >  7:56817/1‑147 (MQ=255)
                                                                                                                 gcgcCAAGCGCGGCATTACGGCCGTCGGCAGCGACGTGGGGTTTGCGCATGAGGTGCAAGGTACAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAgtg                                   <  3:220950/149‑3 (MQ=255)
                                                                                                                   gcCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAg                                                                                      >  3:303328/1‑96 (MQ=255)
                                                                                                                   gcCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAg                                                                                      <  4:303328/96‑1 (MQ=255)
                                                                                                                    ccAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAggggg                                 >  7:99570/1‑148 (MQ=255)
                                                                                                                     cAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGc                                >  3:204550/1‑148 (MQ=255)
                                                                                                                      aaGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCa                               >  7:322322/1‑148 (MQ=255)
                                                                                                                      aaGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCa                               >  2:357038/1‑148 (MQ=255)
                                                                                                                           cgGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACcgc                         <  8:322322/149‑1 (MQ=255)
                                                                                                                           cgGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACcgc                         <  8:14815/149‑1 (MQ=255)
                                                                                                                               aTTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCGt                       >  5:15880/1‑147 (MQ=255)
                                                                                                                                    ggCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCGTCAGCAcc                <  1:357038/149‑1 (MQ=255)
                                                                                                                                     gCCGTCGGCATCGACCTGCGGTTAGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGGAGGCTTTCCGACTGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCTTCAGCACCt               <  2:41919/149‑1 (MQ=255)
                                                                                                                                     gCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCcac                                                                                        <  3:109847/76‑1 (MQ=255)
                                                                                                                                     gCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCcac                                                                                        >  4:109847/1‑76 (MQ=255)
                                                                                                                                      ccGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCGTCAGCACCt               >  3:145172/1‑148 (MQ=255)
                                                                                                                                                   ccTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCGTCAGCACCTGATGGTGTAGCgg  >  8:165780/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTCCTCGACCTGGCGTTCGAGTTCTTGCCGGCTGCGCTGGCGCAGGCGTGCCAGGCGCAGGCGTTGGCTGACGAAAAGGGCGGCGAACACCAGGCTCAGCCAGACGGCGGCGGCGCCAAGCGCGGCATTACGGCCGTCGGCAGCGACCTGCGGTTTGCGCAGCAGGTGCAAGGTCCAGTTTTCGCCTT‑CCAACGGCAGGCTTTCCCACAGGTACTCGGTACTACCCTCGGGGCCTTGCACGCGGCTAAGGGTGCTGTAGGGGGCAAACCGCGTCAGCACCTGATGGTGTAGCGG  >  NC_002947/1597735‑1598029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑