| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,629,456 | A→C | 6.2% | *322Y (TAA→TAC) | mucB → | sigma factor AlgU regulator MucB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,629,456 | 0 | A | C | 6.2% | 203.2 / 4.4 | 64 | *322Y (TAA→TAC) | mucB | sigma factor AlgU regulator MucB |
| Reads supporting (aligned to +/- strand): ref base A (31/29); new base C (2/2); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 | |||||||||||
GCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCTT > NC_002947/1629317‑1629600 | gCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCt < 3:97574/149‑1 (MQ=255) ccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCttt > 4:338865/1‑149 (MQ=255) cGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCTCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt < 1:309335/149‑1 (MQ=255) cGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt < 6:178848/149‑1 (MQ=255) cTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGTCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGTGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑Gt < 8:342038/149‑1 (MQ=255) cTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑Gt < 6:200906/149‑1 (MQ=255) cGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAAGCGCGCCATTACTGgcgcgcctgg < 4:193309/143‑5 (MQ=255) cGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAAGCGCGCCATTACTGgcgcgcctgg > 3:193309/1‑139 (MQ=255) tCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgcgcc > 8:224604/1‑126 (MQ=255) tCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgcgcc < 7:224604/127‑2 (MQ=255) ccTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTg > 8:196677/1‑149 (MQ=255) tGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGgc < 4:191118/117‑1 (MQ=255) tGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGgc > 3:191118/1‑117 (MQ=255) aTACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGc > 3:936/1‑139 (MQ=255) aTACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGc < 4:936/139‑1 (MQ=255) acCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGc > 5:282691/1‑149 (MQ=255) acCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATACCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGc > 3:243700/1‑149 (MQ=255) acCCAAGGGCAAAGCGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGc > 8:100209/1‑149 (MQ=255) ccAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCt > 5:64101/1‑149 (MQ=255) cAATGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtttt < 7:330114/109‑1 (MQ=255) aaaGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaatga > 2:316005/1‑148 (MQ=255) aaGTGATGGTGACCGTGGTTGGCGAAATTCCGCGGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaatgaaa < 2:342808/149‑1 (MQ=255) ggTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAg > 8:343122/1‑148 (MQ=255) tGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCt < 1:87514/131‑1 (MQ=255) tGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCt > 2:87514/1‑131 (MQ=255) gACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtt < 3:327337/95‑1 (MQ=255) gACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtt > 4:327337/1‑95 (MQ=255) aCCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTg < 5:297900/118‑1 (MQ=255) aCCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTg > 6:297900/1‑118 (MQ=255) gTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAAt > 8:227578/1‑82 (MQ=255) gTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAAt < 7:227578/82‑1 (MQ=255) tGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGt < 2:291782/113‑1 (MQ=255) ttGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTg > 5:371207/1‑149 (MQ=255) cGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGccga > 6:105136/1‑146 (MQ=255) gAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCtg > 6:365542/1‑118 (MQ=255) gAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCtg < 5:365542/118‑1 (MQ=255) gAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATc > 2:137401/1‑149 (MQ=255) tCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCAttt > 2:26323/1‑138 (MQ=255) tCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCAttt < 1:26323/138‑1 (MQ=255) tCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCg > 5:313048/1‑149 (MQ=255) ccGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCg < 7:100209/148‑1 (MQ=255) cTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCa > 2:266099/1‑148 (MQ=255) cTGCGTAAGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAAGATGCACAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAg > 5:340831/1‑85 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCta < 6:313048/149‑1 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCta > 4:195288/1‑149 (MQ=255) ggCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCta < 7:18746/149‑1 (MQ=255) aCGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTc < 3:195288/149‑1 (MQ=255) cccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTg < 7:196677/149‑1 (MQ=255) tGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTg > 7:314086/1‑146 (MQ=255) tCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAagag > 2:76969/1‑149 (MQ=255) gATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGcc > 8:242455/1‑149 (MQ=255) aTGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCt > 1:314484/1‑149 (MQ=255) ccAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGCCAGGCTCTTCAAAGCCTGACCtaaa < 3:338865/149‑4 (MQ=255) cAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCTCCTCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCtttt < 1:316005/149‑1 (MQ=255) cccAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtg < 8:314086/149‑1 (MQ=255) ccAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgt > 5:91846/1‑149 (MQ=255) cAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgtg > 4:48769/1‑149 (MQ=255) gcgcgcCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAgg < 6:39485/44‑1 (MQ=255) gcgcgcCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAgg > 5:39485/1‑44 (MQ=255) gcgcCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg < 4:243700/149‑1 (MQ=255) gcgcCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg < 1:266099/149‑1 (MQ=255) gcgcCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg < 3:48769/149‑1 (MQ=255) cAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCt > 6:387456/1‑149 (MQ=255) aGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCtt < 2:314484/149‑1 (MQ=255) | GCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGC‑GTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCTT > NC_002947/1629317‑1629600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |