Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,637,336 G→A 6.5% intergenic (+45/+21) pdxJ → / ← PP_1437 pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,637,3360GA 6.5% 177.9 / 2.8 61intergenic (+45/+21)pdxJ/PP_1437pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (31/26);  new base A (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TGAGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTC  >  NC_002947/1637189‑1637482
                                                                                                                                                   |                                                                                                                                                  
tGAGTTGAACATTGGCGATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTCTGGCCGAGATGTAGTCGCTGATCGTGGCGGCTTCGCTCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCTGGCAAGCCCGCGAaga                                                                                                                                                   <  5:221858/149‑1 (MQ=255)
  aGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCTTGCCCGCGAaaag                                                                                                                                                  <  1:213546/148‑1 (MQ=255)
  aGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAagag                                                                                                                                                  <  6:136933/148‑1 (MQ=255)
      gAACTTTGGCCTTGCGCGGGTGGCGCATGCCTGGTTTGGGGGCTTCACGGCTGCTGGGGCCGATATCCTGGCGGTTAGCGGGGCGGCTTCGCGCTTATTGTTGGGCGCCTGTACCGGCCTTTTCGCGGGCTTGCCCGCTAAAAGGccg                                                                                                                                              <  2:173367/148‑2 (MQ=255)
      gAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCa                                                                                                                                              <  5:285730/148‑1 (MQ=255)
      gAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGACTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAg                                                                                                                                             >  1:88386/1‑149 (MQ=255)
       aaCATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGc                                                                                                                                                >  5:160588/1‑145 (MQ=255)
       aaCATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGc                                                                                                                                                <  6:160588/145‑1 (MQ=255)
       aaCATTGGCCATGCGCTGGTGGCGCATGCATTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAg                                                                                                                                             >  5:371677/1‑148 (MQ=255)
           ttGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAg                                                                                                                                        <  4:215736/149‑1 (MQ=255)
               ccATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAggcgg                                                                                                                                    >  3:258235/1‑149 (MQ=255)
                cATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGGTAAGAGGCCAGCACAggcggc                                                                                                                                   <  2:190592/149‑1 (MQ=255)
                             cgcATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCa                                                                                                                      <  1:41710/149‑1 (MQ=255)
                                            tCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAgg                                                                                                                                       <  4:265549/117‑1 (MQ=255)
                                            tCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAgg                                                                                                                                       >  3:265549/1‑117 (MQ=255)
                                               gCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAAc                                                                                                    >  4:229834/1‑149 (MQ=255)
                                               gCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAAc                                                                                                    >  3:123771/1‑149 (MQ=255)
                                                cTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACAcc                                                                                                             <  1:345583/139‑1 (MQ=255)
                                                cTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACAcc                                                                                                             >  2:345583/1‑139 (MQ=255)
                                                 ttCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCg                                                                                                  >  3:324772/1‑149 (MQ=255)
                                                                gCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggg                                                                                    <  2:347674/148‑1 (MQ=255)
                                                                 ccGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGAACACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg                                                                                  >  6:82503/1‑149 (MQ=255)
                                                                  cGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGc                                                                                 <  4:123771/149‑1 (MQ=255)
                                                                   gagaTGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGt                                                                                               <  8:97663/134‑1 (MQ=255)
                                                                   gagaTGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGt                                                                                               >  7:97663/1‑134 (MQ=255)
                                                                   gagaTGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCa                                                                                <  4:324772/149‑1 (MQ=255)
                                                                    agaTGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCGTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAg                                                                               <  6:371677/149‑1 (MQ=255)
                                                                       tGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctg                                                                            <  4:258235/149‑1 (MQ=255)
                                                                        gAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgc                                                                           >  1:249611/1‑149 (MQ=255)
                                                                          aGGCGCTGAACGTGGCGGCTTCGCGCTTATTGTAGGTCGCCTGTACAGGCCTTTTCGCGGGCAAGCACGCGAAGAGGACAGCACAGGCGTCCGATCACTCGGCCGGAAACACCAGGGTAATACGGGTCGGCCCCAGGGGGCCgctgatt                                                                         >  4:265313/1‑146 (MQ=255)
                                                                           ggCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAgg                                                                                                                    >  8:315833/1‑105 (MQ=255)
                                                                           ggCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAgg                                                                                                                    <  7:315833/105‑1 (MQ=255)
                                                                              gcTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCt                                                                     >  8:100192/1‑149 (MQ=255)
                                                                                  aTCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgct                                                                          <  3:349164/140‑1 (MQ=255)
                                                                                  aTCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgct                                                                          >  4:349164/1‑140 (MQ=255)
                                                                                  aTCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGAc                                                                 <  5:330579/149‑1 (MQ=255)
                                                                                      tGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATGACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGAcccgc                                                             >  5:166711/1‑149 (MQ=255)
                                                                                          ggcTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACccgcccgc                                                         <  5:82503/149‑1 (MQ=255)
                                                                                                 cgcTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCaa                                                  <  3:86779/149‑1 (MQ=255)
                                                                                                   cTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg                                                                                  <  7:217029/115‑1 (MQ=255)
                                                                                                   cTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg                                                                                  >  8:217029/1‑115 (MQ=255)
                                                                                                   cTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACt                                                >  7:203568/1‑149 (MQ=255)
                                                                                                      aTTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa                                             <  2:249611/149‑1 (MQ=255)
                                                                                                      aTTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa                                             >  3:97825/1‑149 (MQ=255)
                                                                                                      aTTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa                                             >  3:92653/1‑149 (MQ=255)
                                                                                                              tCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGAc                                     >  4:13751/1‑149 (MQ=255)
                                                                                                                  cTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCa                                 <  2:109313/149‑1 (MQ=255)
                                                                                                                  cTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCa                                 <  2:88386/149‑1 (MQ=255)
                                                                                                                  cTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCa                                 >  1:335367/1‑149 (MQ=255)
                                                                                                                  cTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCa                                 >  5:102022/1‑149 (MQ=255)
                                                                                                                       ccGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATc                            <  6:166711/149‑1 (MQ=255)
                                                                                                                        tggCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCg                           >  7:288673/2‑149 (MQ=39)
                                                                                                                        cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGa                              >  1:274470/1‑146 (MQ=255)
                                                                                                                        cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGa                              <  2:274470/146‑1 (MQ=255)
                                                                                                                          gCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGc                          >  3:155348/1‑148 (MQ=255)
                                                                                                                            ctcttCGCGGGCTTGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaa                       >  4:171635/4‑149 (MQ=255)
                                                                                                                            cTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCcgcccgcc                                                        >  2:127974/1‑116 (MQ=255)
                                                                                                                            cTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCcgcccgcc                                                        <  1:127974/116‑1 (MQ=255)
                                                                                                                              cttCGCGGGCTTGCCCGCGAAAAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaa                       <  5:316541/146‑1 (MQ=255)
                                                                                                                              cttCGCGGGCTTGCCCGCGAAAAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaa                       >  6:316541/2‑147 (MQ=255)
                                                                                                                                          gCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTcc         >  2:75501/1‑149 (MQ=255)
                                                                                                                                               cgAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaaccccaac                <  5:113223/137‑1 (MQ=255)
                                                                                                                                               cgAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaaccccaac                >  6:113223/1‑137 (MQ=255)
                                                                                                                                                 aaGAGGCCAGCACAGGCGGCCGATCACTGGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTc  >  1:215384/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTC  >  NC_002947/1637189‑1637482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑