Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,659,949	A→C	6.3%	D20A (GAC→GCC)	nudL →	NUDIX hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,659,949	0	A	C	6.3%	183.6 / 2.2	61	D20A (GAC→GCC)	nudL	NUDIX hydrolase
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base C (2/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCACCGTGCCGCCGCAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGCCGCCGCGACCCGGAAGACCCGGA  >  NC_002947/1659802‑1660096                                                                                                                                                     |                                                                                                                                                    ggCCACCGTGCCGCCGCAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGAc                                                                                                                                                    <  6:285682/149‑1 (MQ=255)            cgccgcAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACTACCAACCCGCGTCATTGGAAACCGACAGGCGGTTccc                                                                                                                                         <  1:15403/149‑1 (MQ=255)             gccgcAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTcccc                                                                                                                                        >  6:181430/1‑149 (MQ=255)             gccgcAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTcccc                                                                                                                                        <  6:361734/149‑1 (MQ=255)              ccgcAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTcccc                                                                                                                                        >  6:100830/1‑148 (MQ=255)                gcAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGaa                                                                                                                                     >  8:132993/1‑149 (MQ=255)                 cAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAg                                                                                                                                    <  3:250013/149‑1 (MQ=255)                     cgcTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAgcggc                                                                                                                                >  5:25932/1‑149 (MQ=255)                          gggCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTc                                                                                                                            <  5:100830/148‑1 (MQ=255)                             cTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCctct                                                                                                                        >  3:249343/1‑149 (MQ=255)                                 ggTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGc                                                                                                                     <  7:132993/148‑1 (MQ=255)                                 ggTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGc                                                                                                                     <  8:30036/148‑1 (MQ=255)                                 ggTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGc                                                                                                                     <  1:322457/148‑1 (MQ=255)                                 ggTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGc                                                                                                                     <  5:161036/148‑1 (MQ=255)                                  gTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGcc                                                                                                                    >  1:164984/1‑148 (MQ=255)                                   tGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGCCAGGCGGTTCCCCGAAgcggcg                                                                                                                               <  7:58287/136‑1 (MQ=255)                                   tGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAgcggcg                                                                                                                               >  8:58287/1‑136 (MQ=255)                                        cgcgGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTAc                                                                                                              <  5:181430/148‑1 (MQ=255)                                              tttGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGc                                                                                                       >  3:229260/1‑149 (MQ=255)                                                  gTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGaa                                                                                                    <  7:326651/148‑1 (MQ=255)                                                  gTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGaa                                                                                                    <  4:338513/148‑1 (MQ=255)                                                   tGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAgcggcg                                                                                                                               >  6:319256/1‑120 (MQ=255)                                                   tGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAgcggcg                                                                                                                               <  5:319256/120‑1 (MQ=255)                                                      atcatGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCCACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAATCGcc                                                                                               >  5:269130/1‑149 (MQ=255)                                                       tcatGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGccc                                                                                              >  6:148087/1‑149 (MQ=255)                                                        catGCAACACACGACCTGGATACGAGCGTGCGCAATGCAGGAGCGAGATACTTAGCCGAATCAGCAACAACCACCCCGCGTCATTGGAAACCGACAGCCGCTTCCCCGAACCGGCGGACATCTTGCCCATTACCACCAGCGAAcccccc                                                                                              >  1:72993/1‑149 (MQ=255)                                                            cAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACt                                                                                         >  3:281627/1‑149 (MQ=255)                                                              acacAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTgg                                                                                       <  6:25932/149‑1 (MQ=255)                                                                  aGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCtt                                                                                   <  4:281627/149‑1 (MQ=255)                                                                         ggATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTg                                                                            >  1:78055/1‑149 (MQ=255)                                                                               gAGCGTGTGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTgcgcgc                                                                       >  6:331134/1‑148 (MQ=255)                                                                               gAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCAAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTgcgcgc                                                                       >  8:194253/1‑148 (MQ=255)                                                                                 gCGTGTGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCaa                                                                    <  5:331134/149‑1 (MQ=255)                                                                                 gCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGcc                                                                      >  2:230002/1‑147 (MQ=255)                                                                                 gCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGcc                                                                      >  3:35686/1‑147 (MQ=255)                                                                                      cgcAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGc                                                                <  4:229260/148‑1 (MQ=255)                                                                                      cgcAATGCGGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGc                                                                <  5:115995/148‑1 (MQ=255)                                                                                              tGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTAcc                                                       <  2:78055/149‑1 (MQ=255)                                                                                                gA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTAcc                                                       <  4:249343/147‑1 (MQ=255)                                                                                                 a‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCAAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCAc                                                    <  7:194253/149‑1 (MQ=255)                                                                                                    gAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACgg                                                  >  5:90509/1‑149 (MQ=255)                                                                                                      gCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTg                                                >  8:159342/1‑149 (MQ=255)                                                                                                           ttCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGaa                                           <  2:164984/149‑1 (MQ=255)                                                                                                            tCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAg                                          >  6:206533/1‑149 (MQ=255)                                                                                                            tCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAg                                          >  5:248261/1‑149 (MQ=255)                                                                                                              gCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAg                                          >  2:173825/1‑147 (MQ=255)                                                                                                              gCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGt                                         <  1:230002/148‑1 (MQ=255)                                                                                                              gCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGt                                         <  4:157479/148‑1 (MQ=255)                                                                                                              gCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTa                                        <  5:117903/149‑1 (MQ=255)                                                                                                               ccGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAg                                       >  4:157672/1‑149 (MQ=255)                                                                                                                cGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGc                                      <  4:35686/149‑1 (MQ=255)                                                                                                                           ccaccaACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTg                           <  6:90509/149‑1 (MQ=255)                                                                                                                             accaACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACg                                                   <  3:38315/123‑1 (MQ=255)                                                                                                                             accaACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACg                                                   >  4:38315/1‑123 (MQ=255)                                                                                                                                  cccGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGccgccg                    <  5:148087/149‑1 (MQ=255)                                                                                                                                     gcgTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGCCGCCGCGa                 >  8:52924/1‑149 (MQ=255)                                                                                                                                            ggggaaCCGCCTGTCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGCCGCCGCGACCCgg            >  3:31591/5‑147 (MQ=255)                                                                                                                                             gggaaCCGCCTGTCGGTTCCCCGAAGCGGCTGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGt                            <  5:94405/127‑1 (MQ=255)                                                                                                                                             gggaaCCGCCTGTCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGt                            >  6:94405/4‑130 (MQ=255)                                                                                                                                             ggAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGCCGCCGCGACCCGGaa          <  3:157672/148‑1 (MQ=255)                                                                                                                                                    gACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGCCGCCGCGACCCGGAAGACCCGGa  >  2:216973/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGCCACCGTGCCGCCGCAAGCGCTGGGGCTTGGGTGCTTCGCGGCTTTGGTGCATCATGCAACACAGGACCTGGATACGAGCGTGCGCAATGCTGGA‑CGAGCTACTTCGCCGAATGAGCAACCACCAACCCGCGTCATTGGAAACCGACAGGCGGTTCCCCGAAGCGGCGGTCCTCTTGCCCATTACCCGCAGCGAAGCGCCCGAACTGGTCTTGACCCTGCGCGCCAAGGGCCTGTCTACCCACGGTGGCGAAGTAGCCTTTCCCGGTGGCCGCCGCGACCCGGAAGACCCGGA  >  NC_002947/1659802‑1660096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑