Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,704,636	T→A	7.6%	M1M (TTG→ATG) †	PP_1499 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,704,636	0	T	A	7.6%	219.0 / 2.4	79	M1M (TTG→ATG) †	PP_1499	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/43);  new base A (4/3);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGAC  >  NC_002947/1704491‑1704782                                                                                                                                                  |                                                                                                                                                   gaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGc                                                                                                                                                 >  4:366353/1‑149 (MQ=255)       aaCGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCcgcg                                                                                                                                            >  8:212350/1‑148 (MQ=255)       aaCGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTAGCGGGCTTTGCcgcg                                                                                                                                            >  7:130081/1‑148 (MQ=255)          ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTCTTGGCGTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                        <  1:35136/149‑1 (MQ=255)           gcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAagag                                                                                                                                       >  1:7983/1‑149 (MQ=255)              acggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGcc                                                                                                                                    <  3:133953/149‑1 (MQ=255)                ggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCgg                                                                                                                                  <  2:2136/149‑1 (MQ=255)                  cgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCTAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTa                                                                                                                                <  1:279362/149‑1 (MQ=255)                    tGCTGATCGACTGCCCGGCGGCGGGGGTGCTGGCCTACCTGGCCGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTGGGGCAAATGACGGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACa                                                                                                                              <  4:354420/149‑1 (MQ=255)                      cTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAg                                                                                                                             <  4:246988/148‑1 (MQ=255)                        gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGtt                                                                                                                          >  5:319510/1‑149 (MQ=255)                        gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGtt                                                                                                                          >  8:11236/1‑149 (MQ=255)                        gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGtt                                                                                                                          >  6:319349/1‑149 (MQ=255)                          tCGCCAGCCGGGGGGCGGGGGTGCTGGCCTACCTGCCCGACCCGCCCAACTCCGACGTGTGCGTCTATGGGGCGGGTTTCCCGCGCTCGGGAAATTCCCGGTGTTGGCGTCTGGGGGGGCTGGCCGGCTAAGAGGCCGGTACAGGTTTc                                                                                                                        <  8:130081/149‑1 (MQ=255)                             aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGttt                                                                                                                         <  3:296877/145‑1 (MQ=255)                             aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCAGCGAAGAGGCCGGTACAGGttt                                                                                                                         >  4:296877/1‑145 (MQ=255)                                 cccGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACt                                                                                                                 >  3:141858/1‑149 (MQ=255)                                       ggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                           <  7:11236/149‑1 (MQ=255)                                       ggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAACAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                           <  1:31644/149‑1 (MQ=255)                                                  tGGCCTCCCTCGACGACCCGCCCACCTCCCCCCTTTTGGGGGACGTGGCGGGGTTCCTGCGTGCGGTAAATTGCCCGTGTTGGGGTCTTCGGGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGt                                                                                                <  2:333763/149‑1 (MQ=255)                                                  tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTa                                                                                                                   >  5:296809/1‑130 (MQ=255)                                                  tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTa                                                                                                                   <  6:296809/130‑1 (MQ=255)                                                  tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGt                                                                                                >  6:213642/1‑149 (MQ=255)                                                     ccTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCGTAGCGGGCATGACCGCGCAGAGGCCCGGACACGGTTAGTGTAATAAAAAAGAGTTAACGGTGGa                                                                                             >  4:1739/1‑149 (MQ=255)                                                      cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGAc                                                                                            <  3:366353/149‑1 (MQ=255)                                                      cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTACAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTACCGGTGGAc                                                                                            >  7:152339/1‑149 (MQ=255)                                                           tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTg                                                                                                                                                  >  7:362031/1‑90 (MQ=255)                                                           tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTg                                                                                                                                                  <  8:362031/90‑1 (MQ=255)                                                           tGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATa                                                                                       >  1:313966/1‑149 (MQ=255)                                                            ggACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATAc                                                                                      >  7:262880/1‑149 (MQ=255)                                                             gacgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATAc                                                                                      <  6:319510/148‑1 (MQ=255)                                                                gacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAg                                                                                   >  2:4246/1‑148 (MQ=255)                                                                   ccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCaaa                                                                               <  8:110843/149‑1 (MQ=255)                                                                      gCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCa                                                                            >  1:77093/1‑149 (MQ=255)                                                                        cAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTAGCGGGCATTCCCCCGAAGAGGCCGGTACAGGGTTCGTGTACTAGAAAGGAGTTACCGGTGGCCGAATAACAGCAAACCAta                                                                          >  4:156466/1‑148 (MQ=255)                                                                         aaCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATcc                                                                         <  5:224952/149‑1 (MQ=255)                                                                         aaCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATcc                                                                         <  5:213642/149‑1 (MQ=255)                                                                           cTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                        <  3:235101/132‑1 (MQ=255)                                                                           cTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                        >  4:235101/1‑132 (MQ=255)                                                                                     ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAATAGGCCGGTACAGGTTTCgt                                                                                                                      >  3:273462/1‑92 (MQ=255)                                                                                     ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAATAGGCCGGTACAGGTTTCgt                                                                                                                      <  4:273462/92‑1 (MQ=255)                                                                                     ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTAGTAGAAAg                                                                                                           >  1:311249/1‑103 (MQ=255)                                                                                     ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                           <  2:311249/103‑1 (MQ=255)                                                                                        gtcgAAGTGGCGCGGTTCCAGGGCTCGGGCAAATGACCGTGTTGGCGTGTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGagc                                                          <  5:219813/149‑3 (MQ=255)                                                                                        gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGAcc                                                          >  8:51762/1‑149 (MQ=255)                                                                                        gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGAc                                                           <  2:117174/148‑1 (MQ=255)                                                                                           gAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                           >  3:125294/1‑97 (MQ=255)                                                                                           gAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                           <  4:125294/97‑1 (MQ=255)                                                                                             aGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCAtc                                                     <  7:212350/149‑1 (MQ=255)                                                                                                  cgcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgc                                                <  3:1739/149‑1 (MQ=255)                                                                                                   gcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                               >  3:244831/1‑149 (MQ=255)                                                                                                    cgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                              <  2:7983/149‑1 (MQ=255)                                                                                                       ttCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGcac                                           <  3:156466/149‑1 (MQ=255)                                                                                                         acAGCTCTCGTGGAAATGACCGTGTTGGGTTCTTGGAGGTCTTTCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGcaca                                          <  7:334257/147‑1 (MQ=255)                                                                                                           aGCGCTCGGGCAAATGACCGTGTTGGCGTCTTAGCGGGCTTGCCCGCGAAGACGCCCGTACAGGTTTAGTGTAAGAGAAACGAGTTCCCCGTGGACGAAATCCAGCAAACCATCCCCGCCCTGTCCGACCGCATCGGCGCGGcacaccc                                       >  6:4892/1‑146 (MQ=255)                                                                                                             cgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                     <  8:262880/149‑1 (MQ=255)                                                                                                             cgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                     <  1:4246/149‑1 (MQ=255)                                                                                                             cgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                     <  8:152339/149‑1 (MQ=255)                                                                                                               cTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCcgcg                                                                                                                                            <  3:374883/44‑1 (MQ=255)                                                                                                               cTCGGGCAAATGACCGTGTTGGCGTCTTCGCGCGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGcc                                    >  8:44804/1‑148 (MQ=255)                                                                                                                  gggCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCgcgc                                                                     <  4:272298/112‑1 (MQ=255)                                                                                                                  gggCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATc                                <  2:313966/149‑1 (MQ=255)                                                                                                                           aCCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                              <  3:329208/126‑1 (MQ=255)                                                                                                                           aCCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCtt                       <  7:302926/149‑1 (MQ=255)                                                                                                                            ccGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTg                      <  8:2503/149‑1 (MQ=255)                                                                                                                             cGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTTCTAGAAAGGAGTTACCGGTGGACGAATACCAGCAAACCATCCGCCCCCTGTCCCACCGCATCGTCGCGGCACAGCCGCCGATCAGGGTCCTTGa                     >  3:29993/1‑149 (MQ=39)                                                                                                                               tgtTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGAcg                   <  5:319349/149‑1 (MQ=255)                                                                                                                                    gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGt               >  3:138800/1‑148 (MQ=255)                                                                                                                                    gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGt               <  1:9446/148‑1 (MQ=255)                                                                                                                                    gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGt               >  7:181837/1‑148 (MQ=255)                                                                                                                                    gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGt               <  5:276279/148‑1 (MQ=255)                                                                                                                                    gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCCCGCCCTGTCCGAACGCCTCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGt               >  1:336885/1‑148 (MQ=255)                                                                                                                                         ttCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCtt                       <  4:294081/135‑1 (MQ=255)                                                                                                                                         ttCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCtt                       >  3:294081/1‑135 (MQ=255)                                                                                                                                          tCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGAc                                       >  7:358555/1‑118 (MQ=255)                                                                                                                                          tCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGAc                                       <  8:358555/118‑1 (MQ=255)                                                                                                                                            gcgGGATTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGga      <  4:160247/149‑1 (MQ=255)                                                                                                                                             cgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac     <  7:44804/149‑1 (MQ=255)                                                                                                                                                gCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgacgac  <  3:364423/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGAC  >  NC_002947/1704491‑1704782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑