Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,706,336	T→G	5.8%	M134R (ATG→AGG)	PP_1500 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,706,336	0	T	G	5.8%	261.3 / 4.1	82	M134R (ATG→AGG)	PP_1500	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (40/36);  new base G (2/3);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCG  >  NC_002947/1706189‑1706484                                                                                                                                                    |                                                                                                                                                     aCGGCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATg                                                                                                                                                     >  1:277879/1‑149 (MQ=255)     cggTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCt                                                                                                                                                 >  7:274507/1‑149 (MQ=255)            cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgca                                                                                                                                          <  8:137856/149‑1 (MQ=255)               ccAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcag                                                                                                                                         >  5:198907/1‑147 (MQ=255)               ccAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcag                                                                                                                                         <  8:300545/147‑1 (MQ=255)                cAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGcagcag                                                                                                                                      <  4:168777/149‑1 (MQ=255)                 aGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAgg                                                                                                                                     >  5:140714/1‑149 (MQ=255)                   ggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgcg                                                                                                                                   <  6:313605/149‑1 (MQ=255)                   ggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgcg                                                                                                                                   >  3:196088/1‑149 (MQ=255)                    gCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgcgc                                                                                                                                  >  5:238170/1‑149 (MQ=255)                    gCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCAACCAGGTGCTGATGATCGATTACCGAGGATAAGGCTTGTCTCAGGGCCCGCCGAGACTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAAGGCCTGGCTCCAGCAGgcgc                                                                                                                                  >  6:337322/1‑149 (MQ=255)                        cTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGccc                                                                                                                                >  5:150477/1‑147 (MQ=255)                               gTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAg                                                                                                                       >  5:365010/1‑149 (MQ=255)                                 aGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACAGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACAAGGACATTGCTGCAGCCATGGGCTCGATGCAGCAGGCGCCCGAGGTCAAgtg                                                                                                                     >  5:215000/1‑147 (MQ=255)                                  gCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAggg                                                                                                                     >  1:48681/1‑148 (MQ=255)                                      cTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCaa                                                                                                                  <  6:186141/147‑1 (MQ=255)                                           tGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagc                                                                                                                                        <  7:159537/120‑1 (MQ=255)                                           tGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCATGCCGGAGGTGTACCAGGACATTGATGCAGCCAGGACATGGCTgcagc                                                                                                                                        >  8:159537/1‑120 (MQ=255)                                            gCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGTAGGCGCCCGAGGTCAAGGGCAAGCCGCTgg                                                                                                          <  1:330919/149‑1 (MQ=255)                                             ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCGGGACATTGCTGCAGCCATGGCCTg                                                                                                                                                >  1:305180/1‑110 (MQ=255)                                             ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCGGGACATTGCTGCAGCCATGGCCTg                                                                                                                                                <  2:305180/110‑1 (MQ=255)                                             ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                           <  2:277879/147‑1 (MQ=255)                                               ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                       <  4:296959/149‑1 (MQ=255)                                               ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                       >  2:92690/1‑149 (MQ=255)                                                gAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAct                                                                                                      <  6:198907/149‑1 (MQ=255)                                                     gggCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGTTGGTACTGCTgg                                                                                                 <  6:365010/149‑1 (MQ=255)                                                        cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgggg                                                                                               >  8:16894/1‑148 (MQ=255)                                                        cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGc                                                                                              <  3:287119/149‑1 (MQ=255)                                                        cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGc                                                                                              <  2:48681/149‑1 (MQ=255)                                                                  cTGATGATCGATTACCGCGGCTCTGGCTTGTCACAGGGCCAGCCGTGCCTGCCGGTGGTGTACCAGGACATTGCTGCAGCGTTGGTCTGGCTGATTCAGGCGCGCGAGGGCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTggg                                                                                    <  6:215000/149‑1 (MQ=255)                                                                    gatgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                           >  6:299603/1‑124 (MQ=255)                                                                    gatgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                           <  5:299603/124‑1 (MQ=255)                                                                     atgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCgg                                                                                 >  5:228382/1‑149 (MQ=255)                                                                      tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGt                                                                                >  8:197132/1‑149 (MQ=255)                                                                      tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGt                                                                                >  5:226395/1‑149 (MQ=255)                                                                         tCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGc                                                                              >  2:346800/1‑148 (MQ=255)                                                                          cGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCa                                                                            >  7:268251/1‑149 (MQ=255)                                                                            aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCa                                                                                             <  8:91729/130‑1 (MQ=255)                                                                            aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCa                                                                                             >  7:91729/1‑130 (MQ=255)                                                                             ttACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                         <  8:233898/149‑1 (MQ=255)                                                                             ttACCGCGGCTATGGCTTGTCGCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                         <  5:63815/149‑1 (MQ=255)                                                                                ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGcc                                                                       <  4:196088/148‑1 (MQ=255)                                                                                ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGcc                                                                       <  6:238170/148‑1 (MQ=255)                                                                                  gcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcatt                                                                    >  3:134800/1‑149 (MQ=255)                                                                                   cgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattc                                                                   <  4:134800/149‑1 (MQ=255)                                                                                    ggatATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattc                                                                   <  1:135504/145‑1 (MQ=255)                                                                                           gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCt                                                            <  7:16894/148‑1 (MQ=255)                                                                                           gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTg                                                           >  5:317725/1‑149 (MQ=255)                                                                                             ttGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCAATACCTggc                                                         >  5:356346/1‑149 (MQ=255)                                                                                                tctcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcggc                                                      >  3:168415/1‑149 (MQ=255)                                                                                                tctcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcgg                                                       <  7:197132/148‑1 (MQ=255)                                                                                                  tcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGccc                                                    >  4:33690/1‑149 (MQ=255)                                                                                                   cAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCa                                                   >  7:335711/1‑149 (MQ=255)                                                                                                    aGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGcc                                                     <  8:274507/146‑1 (MQ=255)                                                                                                    aGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAc                                                  >  5:178106/1‑149 (MQ=255)                                                                                                      ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                                >  3:135319/1‑149 (MQ=255)                                                                                                          aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGc                                                                                   <  5:75925/110‑1 (MQ=255)                                                                                                          aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGc                                                                                   >  6:75925/1‑110 (MQ=255)                                                                                                          aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                            >  5:134368/1‑149 (MQ=255)                                                                                                              gAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagcagc                                        <  3:33690/149‑1 (MQ=255)                                                                                                                 ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                         <  6:329162/113‑1 (MQ=255)                                                                                                                 ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                         >  5:329162/1‑113 (MQ=255)                                                                                                                 ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGt                                      >  2:370565/1‑148 (MQ=255)                                                                                                                     ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAg                                   <  8:268251/147‑1 (MQ=255)                                                                                                                     ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCGGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcg                                 <  8:335711/149‑1 (MQ=255)                                                                                                                        gAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCtt                              >  1:203423/1‑149 (MQ=255)                                                                                                                         aGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTc                             <  4:168415/149‑1 (MQ=255)                                                                                                                                 cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                     >  7:296230/1‑149 (MQ=255)                                                                                                                                 cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                     <  4:317544/149‑1 (MQ=255)                                                                                                                                 cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                     >  5:19814/1‑149 (MQ=255)                                                                                                                                    gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                  <  6:140714/149‑1 (MQ=255)                                                                                                                                    gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                  >  6:344778/1‑149 (MQ=255)                                                                                                                                    gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                  <  1:92690/149‑1 (MQ=255)                                                                                                                                           ctgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGAt           >  2:206851/1‑149 (MQ=255)                                                                                                                                            tgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg          >  7:332269/1‑149 (MQ=255)                                                                                                                                            tgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg          >  7:354284/1‑149 (MQ=255)                                                                                                                                              cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg          <  4:135319/147‑1 (MQ=255)                                                                                                                                              cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCAATACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg          <  6:356346/147‑1 (MQ=255)                                                                                                                                               aGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgtg       >  3:283176/1‑149 (MQ=255)                                                                                                                                               aGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgtg       <  6:134368/149‑1 (MQ=255)                                                                                                                                                gCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcgc                                <  4:60101/123‑1 (MQ=255)                                                                                                                                                gCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcgc                                >  3:60101/1‑123 (MQ=255)                                                                                                                                                    tGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCg  <  5:344778/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     ACGGCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCG  >  NC_002947/1706189‑1706484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑