Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,706,340	C→A	6.0%	A135A (GCC→GCA)	PP_1500 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,706,340	0	C	A	6.0%	270.5 / 4.8	83	A135A (GCC→GCA)	PP_1500	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (40/38);  new base A (2/3);  total (42/41)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

CGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGCCA  >  NC_002947/1706193‑1706488                                                                                                                                                    |                                                                                                                                                     cggTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCt                                                                                                                                                     >  7:274507/1‑149 (MQ=255)        cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgca                                                                                                                                              <  8:137856/149‑1 (MQ=255)           ccAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgcag                                                                                                                                             >  5:198907/1‑147 (MQ=255)           ccAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcag                                                                                                                                             <  8:300545/147‑1 (MQ=255)            cAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGcagcag                                                                                                                                          <  4:168777/149‑1 (MQ=255)             aGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAgg                                                                                                                                         >  5:140714/1‑149 (MQ=255)               ggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgcg                                                                                                                                       <  6:313605/149‑1 (MQ=255)               ggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgcg                                                                                                                                       >  3:196088/1‑149 (MQ=255)                gCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgcgc                                                                                                                                      >  5:238170/1‑149 (MQ=255)                gCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCAACCAGGTGCTGATGATCGATTACCGAGGATAAGGCTTGTCTCAGGGCCCGCCGAGACTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAAGGCCTGGCTCCAGCAGgcgc                                                                                                                                      >  6:337322/1‑149 (MQ=255)                    cTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGccc                                                                                                                                    >  5:150477/1‑147 (MQ=255)                           gTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAg                                                                                                                           >  5:365010/1‑149 (MQ=255)                             aGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACAGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACAAGGACATTGCTGCAGCCATGGGCTCGATGCAGCAGGCGCCCGAGGTCAAgtg                                                                                                                         >  5:215000/1‑147 (MQ=255)                              gCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAggg                                                                                                                         >  1:48681/1‑148 (MQ=255)                                  cTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCaa                                                                                                                      <  6:186141/147‑1 (MQ=255)                                       tGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagc                                                                                                                                            <  7:159537/120‑1 (MQ=255)                                       tGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCATGCCGGAGGTGTACCAGGACATTGATGCAGCCAGGACATGGCTgcagc                                                                                                                                            >  8:159537/1‑120 (MQ=255)                                        gCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGTAGGCGCCCGAGGTCAAGGGCAAGCCGCTgg                                                                                                              <  1:330919/149‑1 (MQ=255)                                         ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCGGGACATTGCTGCAGCCATGGCCTg                                                                                                                                                    >  1:305180/1‑110 (MQ=255)                                         ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCGGGACATTGCTGCAGCCATGGCCTg                                                                                                                                                    <  2:305180/110‑1 (MQ=255)                                         ccGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                               <  2:277879/147‑1 (MQ=255)                                           ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                           >  2:92690/1‑149 (MQ=255)                                           ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                           <  4:296959/149‑1 (MQ=255)                                            gAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAct                                                                                                          <  6:198907/149‑1 (MQ=255)                                                 gggCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGTTGGTACTGCTgg                                                                                                     <  6:365010/149‑1 (MQ=255)                                                    cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgggg                                                                                                   >  8:16894/1‑148 (MQ=255)                                                    cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGc                                                                                                  <  3:287119/149‑1 (MQ=255)                                                    cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGc                                                                                                  <  2:48681/149‑1 (MQ=255)                                                              cTGATGATCGATTACCGCGGCTCTGGCTTGTCACAGGGCCAGCCGTGCCTGCCGGTGGTGTACCAGGACATTGCTGCAGCGTTGGTCTGGCTGATTCAGGCGCGCGAGGGCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTggg                                                                                        <  6:215000/149‑1 (MQ=255)                                                                gatgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                               <  5:299603/124‑1 (MQ=255)                                                                gatgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTg                                                                                                               >  6:299603/1‑124 (MQ=255)                                                                 atgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCgg                                                                                     >  5:228382/1‑149 (MQ=255)                                                                  tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGt                                                                                    >  8:197132/1‑149 (MQ=255)                                                                  tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGt                                                                                    >  5:226395/1‑149 (MQ=255)                                                                     tCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGc                                                                                  >  2:346800/1‑148 (MQ=255)                                                                      cGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCa                                                                                >  7:268251/1‑149 (MQ=255)                                                                        aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCa                                                                                                 <  8:91729/130‑1 (MQ=255)                                                                        aTTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCa                                                                                                 >  7:91729/1‑130 (MQ=255)                                                                         ttACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                             <  8:233898/149‑1 (MQ=255)                                                                         ttACCGCGGCTATGGCTTGTCGCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                             <  5:63815/149‑1 (MQ=255)                                                                            ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGcc                                                                           <  6:238170/148‑1 (MQ=255)                                                                            ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGcc                                                                           <  4:196088/148‑1 (MQ=255)                                                                              gcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcatt                                                                        >  3:134800/1‑149 (MQ=255)                                                                               cgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattc                                                                       <  4:134800/149‑1 (MQ=255)                                                                                ggatATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCcattc                                                                       <  1:135504/145‑1 (MQ=255)                                                                                       gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCt                                                                <  7:16894/148‑1 (MQ=255)                                                                                       gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTg                                                               >  5:317725/1‑149 (MQ=255)                                                                                         ttGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCAATACCTggc                                                             >  5:356346/1‑149 (MQ=255)                                                                                            tctcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcggc                                                          >  3:168415/1‑149 (MQ=255)                                                                                            tctcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTggcgg                                                           <  7:197132/148‑1 (MQ=255)                                                                                              tcAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGccc                                                        >  4:33690/1‑149 (MQ=255)                                                                                               cAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCa                                                       >  7:335711/1‑149 (MQ=255)                                                                                                aGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGcc                                                         <  8:274507/146‑1 (MQ=255)                                                                                                aGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAc                                                      >  5:178106/1‑149 (MQ=255)                                                                                                  ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                                    >  3:135319/1‑149 (MQ=255)                                                                                                      aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGc                                                                                       <  5:75925/110‑1 (MQ=255)                                                                                                      aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGc                                                                                       >  6:75925/1‑110 (MQ=255)                                                                                                      aGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                                >  5:134368/1‑149 (MQ=255)                                                                                                          gAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagcagc                                            <  3:33690/149‑1 (MQ=255)                                                                                                             ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                             <  6:329162/113‑1 (MQ=255)                                                                                                             ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATgg                                                                             >  5:329162/1‑113 (MQ=255)                                                                                                             ccTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGt                                          >  2:370565/1‑148 (MQ=255)                                                                                                                 ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAg                                       <  8:268251/147‑1 (MQ=255)                                                                                                                 ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCGGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcg                                     <  8:335711/149‑1 (MQ=255)                                                                                                                    gAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCtt                                  >  1:203423/1‑149 (MQ=255)                                                                                                                     aGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTc                                 <  4:168415/149‑1 (MQ=255)                                                                                                                             cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                         <  4:317544/149‑1 (MQ=255)                                                                                                                             cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                         >  7:296230/1‑149 (MQ=255)                                                                                                                             cAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCt                         >  5:19814/1‑149 (MQ=255)                                                                                                                                gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                      >  6:344778/1‑149 (MQ=255)                                                                                                                                gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                      <  6:140714/149‑1 (MQ=255)                                                                                                                                gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                      <  1:92690/149‑1 (MQ=255)                                                                                                                                       ctgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGAt               >  2:206851/1‑149 (MQ=255)                                                                                                                                        tgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg              >  7:332269/1‑149 (MQ=255)                                                                                                                                        tgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg              >  7:354284/1‑149 (MQ=255)                                                                                                                                          cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg              <  4:135319/147‑1 (MQ=255)                                                                                                                                          cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCAATACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg              <  6:356346/147‑1 (MQ=255)                                                                                                                                           aGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgtg           >  3:283176/1‑149 (MQ=255)                                                                                                                                           aGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgtg           <  6:134368/149‑1 (MQ=255)                                                                                                                                            gCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcgc                                    <  4:60101/123‑1 (MQ=255)                                                                                                                                            gCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgcgc                                    >  3:60101/1‑123 (MQ=255)                                                                                                                                                tGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCg      <  5:344778/149‑1 (MQ=255)                                                                                                                                                  gCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGc    <  6:178106/149‑1 (MQ=255)                                                                                                                                                    cTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGCCa  <  1:370565/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGGCCA  >  NC_002947/1706193‑1706488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑