Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,712,952 T→A 10.5% intergenic (+35/‑105) adk → / → tsaB adenylate kinase/endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,712,9520TA10.5% 150.0 / 8.1 57intergenic (+35/‑105)adk/tsaBadenylate kinase/endopeptidase
Reads supporting (aligned to +/- strand):  ref base T (23/28);  new base A (4/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01

TACCAGAAGCTGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCT  >  NC_002947/1712810‑1713096
                                                                                                                                              |                                                                                                                                                
tACCAGAAGCTGTCGGCTGCCAATGGCGGCAAGCCGAACTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGc                                                                                                                                            >  3:141725/1‑149 (MQ=255)
  ccAGAAGCTGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCggg                                                                                                                                             <  5:312240/146‑1 (MQ=255)
   cAGAAGCTGTCGGCTGCCAATGGCGGCAAGCGGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGt                                                                                                                                         >  1:133097/1‑149 (MQ=255)
    aGAAGCTGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGtt                                                                                                                                        >  6:323234/1‑149 (MQ=255)
          tGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGcc                                                                                                                                           <  8:96427/140‑1 (MQ=255)
          tGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGcc                                                                                                                                           >  7:96427/1‑140 (MQ=255)
          tGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGtt                                                                                                                                  >  4:360038/1‑149 (MQ=255)
          tGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGtt                                                                                                                                  >  1:296449/1‑149 (MQ=255)
           gTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGttt                                                                                                                                 >  6:172181/1‑149 (MQ=255)
            tCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACCGCCCGCATGCGGGCCGTTGTAGTTTg                                                                                                                                >  2:198987/1‑149 (MQ=255)
              ggCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTa                                                                                                                              <  2:296449/149‑1 (MQ=255)
               gCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAg                                                                                                                             >  3:92493/1‑149 (MQ=255)
                    cAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCg                                                                                                                         >  5:179647/1‑148 (MQ=255)
                     aaTGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTGCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGca                                                                                                                       <  6:288006/149‑1 (MQ=255)
                         gcggcAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAgc                                                                                                                   <  1:198987/149‑1 (MQ=255)
                         gcggcAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAgc                                                                                                                   <  7:297050/149‑1 (MQ=255)
                           ggcAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAgcgc                                                                                                                 <  7:271662/149‑1 (MQ=255)
                           ggcAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAgcgc                                                                                                                 >  1:141687/1‑149 (MQ=255)
                             cAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGccc                                                                                                               <  4:92493/149‑1 (MQ=255)
                                   gAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAg                                                                                                         <  2:133097/149‑1 (MQ=255)
                                   gAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAg                                                                                                         <  3:116158/149‑1 (MQ=255)
                                     aGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGa                                                                                                       <  7:305024/149‑1 (MQ=255)
                                            ccACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAAcg                                                                                                >  3:89693/1‑149 (MQ=255)
                                               caTCGAAGGTGTCGTCTCGGTTGAAGCCATAACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACCGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCCCACACCGCCCCCACAGGACACAAccccg                                                                                             >  4:149044/1‑149 (MQ=255)
                                               caTCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAAcgccg                                                                                             <  6:179647/149‑1 (MQ=255)
                                                 tCGAAGGTGTCGGCTCGGTTGAAGCCATCAACGCCAAGGTCCTGGCAGACCTGAGCTGATCCGGCGCCTGACGCACCTCCACAACCGCCCGCTTGAGGCACGTTGTCGTTTGTACGCCGCACAGCGCCCCCTCAGGACACAACGCCgct                                                                                           >  8:165914/1‑149 (MQ=255)
                                                     aGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGtt                                                                                                                                        >  7:14028/1‑100 (MQ=255)
                                                     aGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGtt                                                                                                                                        <  8:14028/100‑1 (MQ=255)
                                                     aGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCAAGCGGGCCGTTGt                                                                                                                                      >  1:275706/1‑102 (MQ=255)
                                                     aGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCAAGCGGGCCGTTGt                                                                                                                                      <  2:275706/102‑1 (MQ=255)
                                                        tgtCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGctc                                                                                    >  1:362792/1‑149 (MQ=255)
                                                         gtCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGctct                                                                                   <  8:85067/149‑1 (MQ=255)
                                                          tCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCta                                                                                  >  3:110238/1‑149 (MQ=255)
                                                            ggCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCtat                                                                                 <  5:172181/148‑1 (MQ=255)
                                                                         ccATCACCGCCAAGGTCCTGGCAGGCATGAGCTGATCCGTAGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCGCCCTCAGGAGACAACGCGGCTGCTGCTCTATTCTGCCGCTCtttt                                                                   <  7:165914/149‑1 (MQ=255)
                                                                         ccATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCtttt                                                                   <  5:323234/149‑1 (MQ=255)
                                                                           aTCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTGAGGACACAACGCCGCTGCTGCTCTATAAGGCCGCTCgtttt                                                                  >  7:123228/1‑148 (MQ=255)
                                                                                       gTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCACAGGACACAAca                                                                                                >  1:337718/1‑105 (MQ=255)
                                                                                           tGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCtata                                                                                <  5:220331/118‑1 (MQ=255)
                                                                                               aGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGt                                             <  2:141687/149‑1 (MQ=255)
                                                                                                 cccTGAGCTGGTCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGctct                                                                                   <  4:82073/109‑1 (MQ=255)
                                                                                                 cccTGAGCTGGTCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGctct                                                                                   >  3:82073/1‑109 (MQ=255)
                                                                                                  ccTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGTTGTTGTCGTTTGTAGGCCGCTCAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCg                                          <  1:121207/149‑1 (MQ=255)
                                                                                                      aGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGAt                                        <  4:141725/147‑1 (MQ=255)
                                                                                                          gATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGaccac                                  >  2:219018/1‑149 (MQ=255)
                                                                                                           aTCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGaccacc                                 <  1:219018/149‑1 (MQ=255)
                                                                                                               ggCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCctgc                             <  4:110238/149‑1 (MQ=255)
                                                                                                                  gcCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTgg                          <  4:89693/149‑1 (MQ=255)
                                                                                                                            aaacGACAACGGCCCGCAAGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATAc                >  2:59865/4‑149 (MQ=255)
                                                                                                                                gACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCg              <  4:16769/147‑1 (MQ=255)
                                                                                                                                gACAACGGCCCGCAAGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGcc            >  7:275296/1‑149 (MQ=255)
                                                                                                                                   aaCGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCAcc         >  5:297211/1‑149 (MQ=255)
                                                                                                                                     cGGCCCGCAAGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCg        >  8:1361/1‑148 (MQ=255)
                                                                                                                                      ggCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGaa      <  3:360038/149‑1 (MQ=255)
                                                                                                                                      ggCCCGCAAGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGaa      <  1:59865/149‑1 (MQ=255)
                                                                                                                                       gCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAg     >  3:307044/1‑149 (MQ=255)
                                                                                                                                          cGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCt  <  4:307044/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TACCAGAAGCTGTCGGCTGCCAATGGCGGCAAGCCGAAGTACAGCCACATCGAAGGTGTCGGCTCGGTTGAAGCCATCACCGCCAAGGTCCTGGCAGCCCTGAGCTGATCCGGCGCCTGACGCACCTCGACAACGGCCCGCTTGCGGGCCGTTGTCGTTTGTAGGCCGCACAGCGCCCCCTCAGGACACAACGCCGCTGCTGCTCTATACTGCCGCTCTTTTTCATTCGCACCTGGATACCCGTTCGATGACCACCCTGCTGGCCCTGGATACCGCCACCGAAGCCT  >  NC_002947/1712810‑1713096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑