Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,715,481 G→T 7.9% A143S (GCG→TCG)  PP_1510 → carboxyvinyl‑carboxyphosphonate phosphorylmutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,715,4810GT 7.9% 201.3 / 5.6 63A143S (GCG→TCG) PP_1510carboxyvinyl‑carboxyphosphonate phosphorylmutase
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base T (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CTTGCCCGAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGC  >  NC_002947/1715333‑1715622
                                                                                                                                                    |                                                                                                                                             
cTTGCCCGAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTg                                                                                                                                               >  2:177118/1‑149 (MQ=255)
   gCCCGAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTgc                                                                                                                                              <  4:297524/147‑1 (MQ=255)
    cccGAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTgcgc                                                                                                                                            <  3:6446/148‑1 (MQ=255)
     ccGAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGcgcg                                                                                                                                           >  5:39364/1‑148 (MQ=255)
       gAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAg                                                                                                                                        >  8:153048/1‑149 (MQ=255)
        aaGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTTCGCGCAGc                                                                                                                                       <  6:23924/149‑1 (MQ=255)
             tgtgCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGc                                                                                                                                   >  3:191411/1‑148 (MQ=255)
             tgtgCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGc                                                                                                                                   >  1:338245/1‑148 (MQ=255)
                 cccAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTc                                                                                                                              <  7:191294/149‑1 (MQ=255)
                 cccAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTc                                                                                                                              >  5:148562/1‑149 (MQ=255)
                 cccAGACCATTCTGCGAGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTc                                                                                                                              <  2:158264/149‑1 (MQ=255)
                  ccAGACCATTCTGCGTGCCGCCGAAATTGGGCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCAAAGCCTGCCATTcc                                                                                                                             >  1:35276/1‑149 (MQ=255)
                    aggccATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTATGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCg                                                                                                                           >  7:108893/4‑149 (MQ=255)
                    aGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCg                                                                                                                           >  7:360521/1‑149 (MQ=255)
                        cATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCa                                                                                                                       >  4:339510/1‑149 (MQ=255)
                         aTTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCAc                                                                                                                      <  1:177118/149‑1 (MQ=255)
                          ttCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTATGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCAcc                                                                                                                     <  8:108893/149‑1 (MQ=255)
                           tCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCt                                                                                                                    >  3:155274/1‑149 (MQ=255)
                            cTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCtt                                                                                                                   >  8:85268/1‑149 (MQ=255)
                               cGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTtgtg                                                                                                                <  1:143573/149‑1 (MQ=255)
                               cGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTtgt                                                                                                                 <  7:85268/148‑1 (MQ=255)
                                gTGCCGCCGAAATGGGCCTGGGGGGCGGCGCCATCGAAGATGCCAGGGGCCGCAGTGAGGCGCCCCTCTAGGACGGCGGCGTGGCTGGGGCGCGCGTGCGCGCCGCTGGGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCCCCTTGTgc                                                                                                               <  2:63798/149‑1 (MQ=255)
                                  gccgccGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTgcgc                                                                                                             >  8:26755/1‑149 (MQ=255)
                                     gccgAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTTCGCGCAGc                                                                                                                                       <  8:101333/120‑1 (MQ=255)
                                     gccgAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTTCGCGCAGc                                                                                                                                       >  7:101333/1‑120 (MQ=255)
                                       cgAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCggg                                                                                                        <  2:250029/149‑1 (MQ=255)
                                             tGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGaa                                                                                                   >  1:160426/1‑148 (MQ=255)
                                               gCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGaaaa                                                                                                 <  6:148562/148‑1 (MQ=255)
                                               gCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAAc                                                                                                <  6:227476/149‑1 (MQ=255)
                                               gCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCAAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAAc                                                                                                <  2:35276/149‑1 (MQ=255)
                                                 cTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACct                                                                                              <  3:160801/149‑1 (MQ=255)
                                                    gtggGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACctgc                                                                                            <  5:75031/148‑1 (MQ=255)
                                                         cggcTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACg                                                                                      <  2:338245/149‑1 (MQ=255)
                                                            cTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGAc                                                                                   <  1:3292/149‑1 (MQ=255)
                                                            cTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGAc                                                                                   <  6:39364/149‑1 (MQ=255)
                                                                 tCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATg                                                                              <  2:160426/149‑1 (MQ=255)
                                                                   gAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGa                                                                            <  4:191411/149‑1 (MQ=255)
                                                                    aaGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGAc                                                                           <  2:168631/149‑1 (MQ=255)
                                                                         gCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTcga                                                                      >  5:98778/1‑149 (MQ=255)
                                                                            aGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTcgacga                                                                   <  7:26755/149‑1 (MQ=255)
                                                                             gTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCgacgac                                                                  <  8:360521/149‑1 (MQ=255)
                                                                                     aGTGATGCGCCCACCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCt                                                                                                                    <  5:54338/91‑1 (MQ=255)
                                                                                     aGTGATGCGCCCACCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCt                                                                                                                    >  6:54338/1‑91 (MQ=255)
                                                                                                cATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAg                                               >  8:85445/1‑149 (MQ=255)
                                                                                                  tCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGcc                                             >  1:159109/1‑149 (MQ=255)
                                                                                                     aTGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGc                                              >  6:325016/1‑145 (MQ=255)
                                                                                                     aTGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGc                                              <  5:325016/145‑1 (MQ=255)
                                                                                                        aCTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGcc                                       >  1:53734/1‑149 (MQ=255)
                                                                                                        aCTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGcc                                       <  6:98778/149‑1 (MQ=255)
                                                                                                            cGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGc                                              <  1:333839/138‑1 (MQ=255)
                                                                                                            cGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGc                                              >  2:333839/1‑138 (MQ=255)
                                                                                                                  ggCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTg                             >  5:467/1‑149 (MQ=255)
                                                                                                                    cTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGcc                           >  5:275892/1‑149 (MQ=255)
                                                                                                                               gTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTAt                                          >  5:190263/1‑123 (MQ=255)
                                                                                                                               gTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTAt                                          <  6:190263/123‑1 (MQ=255)
                                                                                                                                     gccgcTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGcccc          >  2:364712/1‑149 (MQ=255)
                                                                                                                                       cgcTGTGCAGGCTGCGCGAAGCCTGCCATTGCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCg        >  7:166625/1‑149 (MQ=255)
                                                                                                                                         cTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCggg      <  1:364712/149‑1 (MQ=255)
                                                                                                                                           tggCAGGCTTCGCGCAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCgg       >  2:97788/3‑146 (MQ=255)
                                                                                                                                           tggCAGGCTTCGCGCAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCgg       <  1:97788/144‑1 (MQ=255)
                                                                                                                                            tgCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTg   >  8:53828/1‑149 (MQ=255)
                                                                                                                                             gCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTgc  >  6:220961/1‑149 (MQ=255)
                                                                                                                                               aGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGGAGAAAACCTGCTGCACGGACGCATGGACCTcgac                                                                     >  1:266010/1‑80 (MQ=255)
                                                                                                                                               aGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTcgac                                                                     <  2:266010/80‑1 (MQ=255)
                                                                                                                                                 gCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCt                                                    <  6:58567/95‑1 (MQ=255)
                                                                                                                                                 gCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCt                                                    >  5:58567/1‑95 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CTTGCCCGAAGACTGTGCCCAGACCATTCTGCGTGCCGCCGAAATTGGCCTGGTGGGCGGCTCCATCGAAGATGCCAGTGGCCGCAGTGATGCGCCCATCTATGACTTCGGCTTGGCTGTGGAGCGCGTGCGCGCCGCTGTGCAGGCTGCGCGAAGCCTGCCATTCCCGTTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGC  >  NC_002947/1715333‑1715622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑