Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,741,930 C→G 10.2% intergenic (‑18/+66) PP_1538 ← / ← PP_1539 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,741,9300CG10.2% 131.7 / 6.5 49intergenic (‑18/+66)PP_1538/PP_1539hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base G (3/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

GGTGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTT  >  NC_002947/1741793‑1742074
                                                                                                                                         |                                                                                                                                                
ggTGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCgg                                                                                                                                         <  6:20666/147‑1 (MQ=255)
ggTGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCgg                                                                                                                                         <  3:53546/147‑1 (MQ=255)
  tGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCGATATGGCGGCa                                                                                                                                       >  5:5931/1‑147 (MQ=255)
    aCTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCGATATGGCGGCaaagg                                                                                                                                   <  6:5931/149‑5 (MQ=255)
    aCTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCGATATGGCGGCaaagg                                                                                                                                   <  6:57217/149‑5 (MQ=255)
    aCTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTg                                                                                                                                    <  4:44455/148‑1 (MQ=255)
        aaGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGaa                                                                                                                                  >  7:276078/1‑146 (MQ=255)
        aaGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATa                                                                                                                                <  8:239517/148‑1 (MQ=255)
            gggAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTg                                                                                                                           >  8:27497/1‑149 (MQ=255)
              gAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTg                                                                                                                           >  5:194951/1‑147 (MQ=255)
                   ccTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAggg                                                                                                                    >  4:123688/1‑149 (MQ=255)
                       gCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATAGCGGCAGTGAATAGCGTGGAGAGGGGTTa                                                                                                                >  6:306141/1‑149 (MQ=255)
                              cGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggg                                                                                                         <  3:123688/149‑1 (MQ=255)
                                   ttCGGGTGACTGGCTTCCGGCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggggtggg                                                                                                    <  8:276078/148‑1 (MQ=255)
                                   gTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGACAGTGAATAGAGTGGAGAGGGGTTACAGCggggtggg                                                                                                    <  6:142275/149‑1 (MQ=255)
                                      gggTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggggtggggt                                                                                                  >  6:39101/1‑148 (MQ=255)
                                      gggTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTa                                                                                                 <  6:143101/149‑1 (MQ=255)
                                        gTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACa                                                                                               <  7:27497/149‑1 (MQ=255)
                                        gTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCGGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGGATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTa                                                                                                 >  5:360453/1‑147 (MQ=255)
                                           aCTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGAt                                                                                            <  6:185115/149‑1 (MQ=255)
                                                     gTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTggg                                                                                  <  3:228877/149‑1 (MQ=255)
                                                         ttCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGga                                                                                                                         <  6:386731/106‑1 (MQ=255)
                                                         ttCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGga                                                                                                                         >  5:386731/1‑106 (MQ=255)
                                                           ccAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGtt                                                                               >  2:218948/1‑146 (MQ=255)
                                                            ccggttGGCTCGGACCTTCGGCCCGCTGAACTGGTTGGGGCGTTCCTTTGCTGTAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATTGAGTGGCGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAag                                                                            <  6:360453/143‑1 (MQ=38)
                                                                  ggCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGc                                                                     >  2:291/1‑149 (MQ=255)
                                                                       ggACCATCGGCACGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCg                                                                 <  6:194951/148‑1 (MQ=255)
                                                                        taCCTTCGGCCCGCTGAACAGGATCGGGCGGTCCTTTGCTTGAGACATAGCTTCGCCGTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCggg                                                               <  5:306141/148‑1 (MQ=255)
                                                                                gCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTgcgc                                                       <  5:39101/149‑1 (MQ=255)
                                                                                       gAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCCCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGtt                                                <  4:164260/149‑1 (MQ=255)
                                                                                          caggtgcGGGGGTTCCTTTGCTTCAGACATCGCTGCGCGTTTGCCGCCATATCGCGGCTGTGAATCGAGTGGCGTGGGGTTCCCGGGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTc                                             <  1:291/143‑1 (MQ=255)
                                                                                              aTCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGc                                          >  7:264653/1‑148 (MQ=255)
                                                                                                          tttGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCg                                                  <  3:34384/128‑1 (MQ=255)
                                                                                                          tttGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCg                                                  >  4:34384/1‑128 (MQ=255)
                                                                                                               ttGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTAc                        >  3:364162/1‑149 (MQ=255)
                                                                                                                     cATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGt                   <  4:364162/148‑1 (MQ=255)
                                                                                                                      aTAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggggtggg                                                                                                    >  1:262630/1‑66 (MQ=255)
                                                                                                                      aTAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCggggtggg                                                                                                    <  2:262630/66‑1 (MQ=255)
                                                                                                                      aTAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGAtgtg                                                                                         >  6:293327/1‑77 (MQ=255)
                                                                                                                      aTAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGAtgtg                                                                                         <  5:293327/77‑1 (MQ=255)
                                                                                                                      aTAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGc                 >  4:109032/1‑149 (MQ=255)
                                                                                                                       tAGCTGCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGGGGAGAGGGGTTACAGCGGGGGGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCg                <  4:343378/149‑1 (MQ=255)
                                                                                                                           ttCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTcc            >  7:86068/1‑149 (MQ=255)
                                                                                                                           ttCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTcc            >  2:205213/1‑149 (MQ=255)
                                                                                                                           ttCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGCGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTCCGCAAGCGTTGTCAGCCATGGCGCGGCACTCAACCTGGTGAGGTcc            >  4:221455/1‑149 (MQ=255)
                                                                                                                            tattcactGCCGCGATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCg           >  7:270437/8‑149 (MQ=255)
                                                                                                                                cTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCg        >  4:62352/1‑148 (MQ=255)
                                                                                                                                 actGCCGCGATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGaa      >  1:155315/3‑149 (MQ=255)
                                                                                                                                   tGCCGCCATATCGCGGAAGTTAATAGAGTTGCCTGGGGTTACTGGGGGGTGGGGTACAGAGGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGaa      <  3:221455/147‑1 (MQ=255)
                                                                                                                                     ccgcgATATGGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCtt  <  8:270437/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                
GGTGACTGAAGAGGGAAAGCCTGGCTTAACCGGGCGTCGGGTGACTGTCTTCCGTCCTTCCAGGATGGCTCGGACCATCGGCCCGCTGAACAGGATCGGGCGTTCCTTTGCTTGAGACATAGCTTCGCCTTTGCCGCCATATCGCGGCAGTGAATAGAGTGGAGAGGGGTTACAGCGGGGTGGGGTACAGATGTGCTCTGGGGTTAAGAGCTGGCACCGGGGCGTGCGCAAGCGTTGTCAGCCATGGCGCGGCACTCTACCTGGTGCGGTCCGTCGAACCTT  >  NC_002947/1741793‑1742074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑