Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,786,023 T→A 5.4% intergenic (+39/‑147) rpsB → / → tsf 30S ribosomal protein S2/elongation factor Ts

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,786,0230TA 5.4% 259.9 / 2.7 85intergenic (+39/‑147)rpsB/tsf30S ribosomal protein S2/elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base T (43/37);  new base A (3/2);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTG  >  NC_002947/1785881‑1786167
                                                                                                                                              |                                                                                                                                                
tGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGcccc                                                                                                                                            >  1:53263/1‑149 (MQ=255)
  tACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGcc                                                                                                                                              <  3:128599/145‑1 (MQ=255)
  tACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCt                                                                                                                                          <  7:89822/149‑1 (MQ=255)
    cATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttt                                                                                                                                        >  7:8215/1‑149 (MQ=255)
     aTGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCtttt                                                                                                                                       >  6:233369/1‑149 (MQ=255)
       gACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTg                                                                                                                                     >  8:323962/1‑149 (MQ=255)
       gACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTg                                                                                                                                     >  8:265855/1‑149 (MQ=255)
        aCTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGc                                                                                                                                    <  3:286245/149‑1 (MQ=255)
        aCTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGc                                                                                                                                    >  2:131147/1‑149 (MQ=255)
             gATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttttt                                                                                                                                      >  4:164652/1‑142 (MQ=255)
             gATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttttt                                                                                                                                      <  3:164652/142‑1 (MQ=255)
                ggCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCtt                                                                                                                                         <  8:170158/136‑1 (MQ=255)
                   tGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAAt                                                                                                                         >  8:43314/1‑149 (MQ=255)
                   tGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCttttt                                                                                                                                      <  3:42976/136‑1 (MQ=255)
                   tGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCttttt                                                                                                                                      >  4:42976/1‑136 (MQ=255)
                    gACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACACAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATc                                                                                                                        >  6:389277/1‑149 (MQ=255)
                       gCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctg                                                                                                                     <  2:350600/149‑1 (MQ=255)
                         aGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctgct                                                                                                                   >  5:282281/1‑149 (MQ=255)
                             aTCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGt                                                                                                                 >  8:157646/1‑147 (MQ=255)
                              tCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCtt                                                                                                                              <  5:58584/133‑1 (MQ=255)
                              tCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCtt                                                                                                                              >  6:58584/1‑133 (MQ=255)
                               ccGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCGCCGTTTTGCCACCGTGAAGTCcggctgagagc                                                                                                             >  4:214866/1‑144 (MQ=255)
                               ccGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctgct                                                                                                                   <  3:214866/143‑1 (MQ=255)
                                 gcgGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAATGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCAt                                                                                                           >  1:316886/1‑149 (MQ=255)
                                 gcgGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACACTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGaaa                                                                                                                          <  4:88625/134‑1 (MQ=255)
                                           caacGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGca                                                                                                 >  3:304057/1‑149 (MQ=255)
                                           caacGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGca                                                                                                 <  5:196700/149‑1 (MQ=255)
                                                       cggcACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgc                                                                                     <  4:111846/149‑1 (MQ=255)
                                                        ggcACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgct                                                                                    <  5:233369/149‑1 (MQ=255)
                                                        ggcACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgct                                                                                    <  2:53263/149‑1 (MQ=255)
                                                          cACCGTAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtga                                                                                  <  1:131147/149‑1 (MQ=255)
                                                           aCCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtga                                                                                  >  7:344058/1‑148 (MQ=255)
                                                             cGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat                                                                               <  6:282281/149‑1 (MQ=255)
                                                             cGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat                                                                               >  5:193144/1‑149 (MQ=255)
                                                              gAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat                                                                               >  4:203440/1‑148 (MQ=255)
                                                               aaGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaatga                                                                             <  6:193144/149‑1 (MQ=255)
                                                                  tttACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGaaaa                                                                          <  7:43314/149‑1 (MQ=255)
                                                                  tttACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGaaaa                                                                          >  6:32434/1‑149 (MQ=255)
                                                                  tttACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGaaaa                                                                          >  6:130866/1‑149 (MQ=255)
                                                                      cGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc                                                                      >  1:347457/1‑149 (MQ=255)
                                                                       gCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGCAATGTAAACAGCg                                                                     >  3:36016/1‑149 (MQ=255)
                                                                         tGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATTGGCCTGCTGAAATGAAAACAGCGGa                                                                   >  3:114960/1‑149 (MQ=255)
                                                                           aagaagCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAt                                                                  <  7:323962/148‑1 (MQ=255)
                                                                           aagaagCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAt                                                                  <  4:304057/148‑1 (MQ=255)
                                                                                gCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat                                                                               <  8:9857/130‑1 (MQ=255)
                                                                                gCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat                                                                               >  7:9857/1‑130 (MQ=255)
                                                                                gCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAttt                                                                >  4:2210/1‑145 (MQ=255)
                                                                                gCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGAttt                                                                <  3:2210/145‑1 (MQ=255)
                                                                                    cTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGTCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAAtt                                                        <  7:145438/149‑1 (MQ=255)
                                                                                       cACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGaa                                                     <  2:347457/149‑1 (MQ=255)
                                                                                       cACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCCCAACATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGaa                                                     >  7:166038/1‑149 (MQ=255)
                                                                                       cACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACACAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGa                                                      <  5:389277/148‑1 (MQ=255)
                                                                                        aCCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCTTCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTg                                                               >  7:281383/1‑138 (MQ=255)
                                                                                        aCCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCTTCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTg                                                               <  8:281383/138‑1 (MQ=255)
                                                                                          cttctgctgAGTAATTGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGc                                                  <  5:130866/146‑1 (MQ=255)
                                                                                          ctgctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc                                                                      <  1:250666/129‑1 (MQ=255)
                                                                                          ctgctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc                                                                      >  2:250666/1‑129 (MQ=255)
                                                                                          ctgctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGc                                                  <  7:157646/149‑1 (MQ=255)
                                                                                            gctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGccc                                                >  2:59474/1‑149 (MQ=255)
                                                                                            gctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGccc                                                >  3:17892/1‑149 (MQ=255)
                                                                                              tgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGt                                              >  2:135109/1‑149 (MQ=255)
                                                                                              tgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGCATTTGCAGAATTGAACGCCCGt                                              >  7:48308/1‑149 (MQ=255)
                                                                                                ctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGa                                            >  4:63913/1‑149 (MQ=255)
                                                                                                     tAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGTCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGAc                                       <  7:198092/149‑1 (MQ=255)
                                                                                                          ggCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt                                   <  4:41439/148‑1 (MQ=255)
                                                                                                           gcgcTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTg                                  >  6:73593/1‑148 (MQ=255)
                                                                                                           gcgcTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTg                                  >  3:363133/1‑148 (MQ=255)
                                                                                                               tAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATg                             >  3:75256/1‑149 (MQ=255)
                                                                                                                    tCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGa                        <  7:265855/149‑1 (MQ=255)
                                                                                                                        cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGaaaaa                    <  8:344058/149‑1 (MQ=255)
                                                                                                                            gCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACttt                <  5:354646/149‑1 (MQ=255)
                                                                                                                            gCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACtt                 <  4:17892/148‑1 (MQ=255)
                                                                                                                             cACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTc               >  2:236238/1‑149 (MQ=255)
                                                                                                                              aCGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTcc              <  1:135109/149‑1 (MQ=255)
                                                                                                                              aCGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTc               >  2:96885/1‑148 (MQ=255)
                                                                                                                                gCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCaa            >  1:158847/1‑149 (MQ=255)
                                                                                                                                 cAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTcc              >  1:330021/1‑146 (MQ=255)
                                                                                                                                  aaaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgc                                                                                     <  6:16948/74‑1 (MQ=255)
                                                                                                                                  aaaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgc                                                                                     >  5:16948/1‑74 (MQ=255)
                                                                                                                                  aaaaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCaa            >  6:13148/1‑147 (MQ=255)
                                                                                                                                  aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCaa            >  6:171076/1‑147 (MQ=255)
                                                                                                                                        ggggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGAc                                       <  7:289283/114‑1 (MQ=255)
                                                                                                                                        ggggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGAc                                       >  8:289283/1‑114 (MQ=255)
                                                                                                                                         gggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGAtttt   <  4:363133/149‑1 (MQ=255)
                                                                                                                                           gCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTg  >  6:188226/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTG  >  NC_002947/1785881‑1786167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑