Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,933,664 G→T 7.3% R30R (CGC→CGA)  minD ← MinC‑MinD‑MinE system ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,933,6640GT 7.3% 173.3 / 3.5 55R30R (CGC→CGA) minDMinC‑MinD‑MinE system ATPase
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

CTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAG  >  NC_002947/1933526‑1933809
                                                                                                                                           |                                                                                                                                                 
cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGc                                                                                                                                           >  4:168541/1‑147 (MQ=255)
cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGa                                                                                                                                         <  3:252277/149‑1 (MQ=255)
cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGa                                                                                                                                         <  4:176579/149‑1 (MQ=255)
 ttGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCgcg                                                                                                                                        >  2:352218/1‑147 (MQ=255)
   gATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAg                                                                                                                                        <  3:64289/147‑1 (MQ=255)
    aTCCGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGGCTTGTGGCCTCGCACTGCGCGGcc                                                                                                                                     <  4:291793/149‑1 (MQ=255)
      cAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCACGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCgg                                                                                                                                   >  2:162375/1‑149 (MQ=255)
       aGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCTCGCACTGCGCGGccaca                                                                                                                                  <  1:352218/149‑4 (MQ=255)
          gCCTGTTGCAGGTTTGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTAcc                                                                                                                               <  7:168203/149‑1 (MQ=255)
          gCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTAc                                                                                                                                >  4:59678/1‑148 (MQ=255)
           ccTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTAcc                                                                                                                               >  3:333034/1‑148 (MQ=255)
             tGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                            >  8:185778/1‑149 (MQ=255)
               ttGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                            <  7:185778/147‑1 (MQ=255)
               ttGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAAt                                                                                                                            <  7:167074/147‑1 (MQ=255)
                     gTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGgcgc                                                                                                                    >  8:306967/1‑149 (MQ=255)
                         gCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTgg                                                                                                                 <  3:168541/148‑1 (MQ=255)
                          cTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTa                                                                                                               <  3:59678/149‑1 (MQ=255)
                                    gACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGTGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGc                                                                                                     >  3:24001/1‑149 (MQ=255)
                                    gACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGc                                                                                                     >  6:258754/1‑149 (MQ=255)
                                     aCCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGc                                                                                                     <  1:230936/148‑1 (MQ=255)
                                      ccACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATgg                                                                                                                          >  2:118664/1‑126 (MQ=255)
                                       cACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCca                                                                                                  >  1:306/1‑149 (MQ=255)
                                          gTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGAGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCcaca                                                                                                >  1:94492/1‑149 (MQ=255)
                                                  aGTCGTACACCTCGCGGAGTTCGCATCCCATTATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGAGGGTCTTGTTGCAGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTg                                                                                       <  5:298760/149‑1 (MQ=255)
                                                   gTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGc                                                                                      >  3:17329/1‑149 (MQ=255)
                                                   gTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGc                                                                                      <  7:306967/149‑1 (MQ=255)
                                                   gTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGc                                                                                      >  5:310705/1‑149 (MQ=255)
                                                         caccacGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                 <  2:306/148‑1 (MQ=255)
                                                            cacGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTaa                                                                             >  2:339782/1‑149 (MQ=255)
                                                            cacGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCACCACCCCCCTTGCCGGAAGTaa                                                                             >  2:335953/1‑149 (MQ=255)
                                                                cggcgTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATggcggc                                                                                                                      <  3:41388/104‑1 (MQ=255)
                                                                cggcgTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATggcggc                                                                                                                      >  4:41388/1‑104 (MQ=255)
                                                                           gCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTgg                                                              <  4:333034/149‑1 (MQ=255)
                                                                            cccATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                             >  3:371462/1‑149 (MQ=255)
                                                                             ccATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCtt                                                                <  4:208353/145‑1 (MQ=255)
                                                                             ccATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCtt                                                                >  3:208353/1‑145 (MQ=255)
                                                                                tGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAg                                                         >  8:327980/1‑149 (MQ=255)
                                                                                   tCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGgtg                                                      >  2:44474/1‑149 (MQ=255)
                                                                                     aGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGTGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGgtgt                                                     <  4:24001/148‑1 (MQ=255)
                                                                                       gTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGttt                                                   >  6:235573/1‑148 (MQ=255)
                                                                                          gAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAcc                                               <  6:310705/149‑1 (MQ=255)
                                                                                                tACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                             >  3:253612/1‑130 (MQ=255)
                                                                                                tACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                             <  4:253612/130‑1 (MQ=255)
                                                                                                   gCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTaaaaa                                       >  4:376514/1‑149 (MQ=255)
                                                                                                     aGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATa                                     <  2:94492/149‑1 (MQ=255)
                                                                                                        cccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTc                                  <  7:327980/149‑1 (MQ=255)
                                                                                                          cACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCgg                                <  4:371462/149‑1 (MQ=255)
                                                                                                          cACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCgg                                <  4:17329/149‑1 (MQ=255)
                                                                                                                gAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACt                          >  6:47036/1‑149 (MQ=255)
                                                                                                                   gTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGt                       <  1:162375/149‑1 (MQ=255)
                                                                                                                                gTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTg                                                               <  1:275345/96‑1 (MQ=255)
                                                                                                                                gTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTg                                                               >  2:275345/1‑96 (MQ=255)
                                                                                                                                      cggCCTCGCACTGCGCGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGaaa     >  1:235804/2‑148 (MQ=255)
                                                                                                                                        gCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTTGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAg  >  2:31307/1‑149 (MQ=255)
                                                                                                                                        gCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTTCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATa   <  5:258754/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
CTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCGTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAG‑GTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAG  >  NC_002947/1933526‑1933809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑