| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,967,197 | A→G | 9.4% | intergenic (+67/+52) | PP_1762 → / ← yciK | hypothetical protein/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,967,197 | 0 | A | G | 9.4% | 136.8 / 4.2 | 55 | intergenic (+67/+52) | PP_1762/yciK | hypothetical protein/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (22/26); new base G (3/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01 | |||||||||||
GTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCGGGTTGTTC > NC_002947/1967058‑1967345 | gttcctgGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTTCCCGCTACCCCAGGATCACCGCCACCCTCAAGGCTTGCGCcct > 5:298515/1‑149 (MQ=255) ttcctgGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGccc < 4:274435/147‑1 (MQ=255) ttcctgGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGccc < 7:220159/147‑1 (MQ=255) ccTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGc > 4:192435‑M2/1‑147 (MQ=255) cTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcct < 6:388296‑M2/149‑4 (MQ=255) tGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTTcctg < 6:377469‑M2/149‑5 (MQ=255) aGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgg > 4:101472‑M2/1‑142 (MQ=255) aGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtg > 5:388296‑M2/1‑142 (MQ=255) gcTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCGCCCACAGGGTCCCCGCCACCCACAAGGATTGCGCCCTGccggggggag < 3:96615‑M2/149‑11 (MQ=255) catgcaGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCCCAGGCTCCCCGCCACCCTCAAGGATTGCGCCCGGgcggtgggagccgg < 5:145997‑M2/149‑15 (MQ=255) catgcaGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGCAGGGCGCAATCCTTGAGGGTTGCGGTGATcctgtgggagcgg > 5:107299‑M2/1‑135 (MQ=255) aGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAATCCTTGAGGGTTgcgg > 1:346069/1‑125 (MQ=38) aGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAATCCTTGAGGGTTgcgg < 2:346069/126‑2 (MQ=38) ggAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgg < 5:5123‑M2/139‑14 (MQ=255) ggAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgg > 6:5123‑M2/1‑126 (MQ=255) gacgggCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTCCCCGGGCTTGCCCGCTCCCACCGGATCACCGCAACCCTCAAGGATGGCGCCCTGCCTGTGGGAGCGGGCAAGCCcgc < 6:224502/145‑1 (MQ=39) gtgccgcccgcAAGGCCGTAGGCGGCCGGGGTTGGCGGCGGGCGGAGCGCTTCCCTGTCCCGGCCTCTTCGCGGGCTTGCCCGCCCCCACCGGATCCCCGCAACCCTCACGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaa > 8:271360‑M2/5‑123 (MQ=255) gcgcCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCACGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagag > 5:110767‑M2/1‑120 (MQ=255) gcgcCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGgcggtgggagcgggccagcccgcgaa < 7:271360‑M2/146‑27 (MQ=255) gCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAATCCTTGAGGGTTgcg < 4:176152/109‑1 (MQ=37) gCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAATCCTTGAGGGTTgcg > 3:176152/1‑109 (MQ=37) ggCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCACAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAg > 6:73641/1‑149 (MQ=255) gtcgggggcggggGTTGGGTGGGGGCGGAGCGCTTTCCTGACCCGCCCTCTTCGCGGGCTTGCCCGCTCCCACCGCATCCCCCAACCCCTCATGGATTGCGCCCTGCCTGGGGGCGGGGGAACGCCCGCGAAGAGGCCGATGCAGGttt < 4:96615/143‑1 (MQ=255) gcGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTg < 5:73641/149‑1 (MQ=255) gTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATAACCGCCACCCTCAAGGATTGCGCCCTGGCTGTGGGAGCCGGCAAGCCCGCGAACAGGCCGATGCAGGTTTATTGGGCGTTGaa > 5:300242/1‑149 (MQ=38) gtGCGGGCTGATCGCTTTCCGTTACCGGCCTCTTCGCGGGCTTGCCGGCTCCCACAGTATCACCGAAACCCTCATGTATTGCGCCCTGCGTGTGGGAGGGGGCATGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCt < 8:177289/148‑1 (MQ=38) tGATCGCTTTCCTGTACCGGCCTCTGCGCGGGCTTGCCCGCTCCCACTGTATCACCGCAACCCTCAAGGTTTGCGCCCTGCCTGTGGGTGGGGGCATGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTc < 6:156122/149‑1 (MQ=38) gATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCCCAGGATCCCCGCAACCCGGAAGGATTGCGCCCGGCCTGGGGGAGCGGGCCAGCCCGCGCAGAGGCCGCTGCAGGTTTTTTGGGCGTTGAACGCCTGCCCGGTcc > 7:353827/1‑148 (MQ=37) tgcGCTTTCCTGTCCCGGCCGCTTCGCGGCCTTGCCCGGTCCCCCCGCATCACCCCACCCCTCACGTATTGCGCCCGGCCTTGGGGTGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCa < 6:298515/146‑1 (MQ=255) aTCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCa < 5:377469/148‑1 (MQ=255) cccGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCACCCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGt < 3:262236/116‑1 (MQ=255) aCCGGCCTCTTCGCGGGCTTTCCCGCTCCCACAGGATAACCCCAACCCTCAAGGATTGCGCCCTGCCGGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGCTGCAGGTTTATTGGGGGt > 4:262236/1‑117 (MQ=25) gcgGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATcagg > 3:108602/1‑149 (MQ=255) gcgGGCTTGCCCGCTCACACAGGATCACCGCAACCCTAAAGGATTGCGCCCTGCCTGGGGGAGCGGGCAAGCCCGCGAAGAGGCCGATTCAGGTTTATTGTGCGTTGAACGCCTTCCCGTTCACCCCAGTGCAGTCCGGCCCCAAcatg > 4:244924/1‑147 (MQ=37) cgGGCTTGCCCGCTCCCACAGGATCACCCCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGGTTATTGGGCGTTGAACGCCTGCCCGTTCCCCCCTGTGCTGTCCGGGCCCCTcagg > 3:326665/1‑148 (MQ=38) cTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTTGGAGGGGGCAAGCCCGAGAAGAGGCCGATTCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATcaggtacag < 3:244924/149‑1 (MQ=255) gCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATcaggtacaggta > 7:159022/1‑149 (MQ=255) ccGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAggtacaggtaca > 6:214631/1‑149 (MQ=255) cTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagaggccggtacaggaaagcgatcagcccgcagccaacgccggccgcatacggccttgctggcgcccttccagctgcatgcagc < 8:293448‑M2/149‑110 (MQ=255) cTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCg < 8:159022/149‑1 (MQ=255) tCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagaggccgatgcaggaaagcgatcagcccgcagccaacgccggccgcatacggccttgctggcgcccttccagctgcatgcagcg < 6:255285‑M2/149‑111 (MQ=255) cccACCGTATCACCGCACCCCTCAAGGATTGCGCCCTGCCTGGGGGTGGGGGAAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGc < 4:326665/149‑1 (MQ=255) cccACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagaggccggtacaggaaagcgatcagcccgcagccaacgccggccgcatacggccttgctggcgccctgccagctgcatgccgcgc < 4:264699‑M2/149‑112 (MQ=255) ccACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagaggccggtacaggaaagcgatcagcccgcagccaacgccggccgcatacggccttgctggcgcccttccagctgcatgcagcgct < 1:293242‑M2/149‑113 (MQ=255) ccACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCa < 4:108602/149‑1 (MQ=255) tCACCGCAACCCTCAAGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagaggccggtacaggaaagcgatcagcccgcagccaacgccggccgcatacggccttgctggcgcccttccagctgcatgcagcgctccaggaac > 2:293242‑M2/1‑29 (MQ=255) accccaccccACACGGATGCCGCCCTGCGGGTGGCAGCGGGAAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTc < 8:353827/149‑1 (MQ=255) cgaatcccTCAAGTATTGGGCCCTGCGTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTc < 6:300242/142‑1 (MQ=255) cAACCCTCAAGGATTGCGCCCTGcctgtgggagcgggcaagcccgcgaagaggccggtacaggaaagcgatcagcccgcagccaacgccggccgcatacggccttgctggcgcccttccagctgcatgcagcgctccaggaacaggaac > 7:293448‑M2/1‑23 (MQ=255) ccTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCg > 6:375639/1‑149 (MQ=255) tCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCggg > 4:231640/1‑149 (MQ=255) tCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCggg > 6:121433/1‑149 (MQ=255) tCAAGGATTGCGCACTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCggg > 8:12882/1‑149 (MQ=255) cAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCGGgt > 8:200789/1‑149 (MQ=255) aaGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCggg < 5:375639/147‑1 (MQ=255) aTTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCGGGTTGTTc < 3:231640/149‑1 (MQ=255) | GTTCCTGGAGCGCTGCATGCAGCTGGAAGGGCGCCAGCAAGGCCGTATGCGGCCGGCGTTGGCTGCGGGCTGATCGCTTTCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCTCCCACAGGATCACCGCAACCCTCAAGGATTGCGCCCTGCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGATGCAGGTTTATTGGGCGTTGAACGCCTGCCCGTTCACCCCTGTGCTGTCCGGCCCCATCAGGTACAGGTACACCGGCATGATCTCTTCGGGCAGCGGGTTGTTC > NC_002947/1967058‑1967345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |