Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,989,577	A→T	5.2%	H276L (CAC→CTC)	PP_1780 →	mannosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,989,577	0	A	T	5.2%	240.3 / 2.1	77	H276L (CAC→CTC)	PP_1780	mannosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (32/41);  new base T (2/2);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CCTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCG  >  NC_002947/1989433‑1989722                                                                                                                                                 |                                                                                                                                                   ccTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAg                                                                                                                                                <  1:371694/149‑1 (MQ=255)  cTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAgg                                                                                                                                               <  8:99927/149‑1 (MQ=255)        tGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGTGCTGACCCAGCTCCAggttaac                                                                                                                                          <  6:130410/148‑6 (MQ=255)        tGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGccc                                                                                                                                         <  1:74923/149‑1 (MQ=255)            gAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTtgt                                                                                                                                     >  3:103727/1‑149 (MQ=255)                 tGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGAtt                                                                                                                                <  1:362601/149‑1 (MQ=255)                   gggTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTg                                                                                                                              <  6:164759/149‑1 (MQ=255)                    ggTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGAttt                                                                                                                               >  4:353103/1‑147 (MQ=255)                        aCCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGa                                                                                                                             <  4:310677/145‑1 (MQ=255)                        aCCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAgag                                                                                                                          >  3:310677/1‑146 (MQ=255)                          ccTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAg                                                                                                                        >  2:254538/1‑148 (MQ=255)                              gAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAg                                                                                                                                            <  8:242990/124‑1 (MQ=255)                              gAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAg                                                                                                                                            >  7:242990/1‑124 (MQ=255)                                   ccTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGc                                                                                                              <  5:240332/149‑1 (MQ=255)                                         tGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATc                                                                                                        >  4:155050/1‑149 (MQ=255)                                           aGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATcgc                                                                                                      >  5:286493/1‑149 (MQ=255)                                             cgcTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCgcgc                                                                                                    >  4:98239/1‑149 (MQ=255)                                                tATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCg                                                                                                  >  5:378701/1‑148 (MQ=255)                                                    aagaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTg                                                                                             >  4:24164/1‑149 (MQ=255)                                                     agaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTg                                                                                             >  8:33134/1‑148 (MQ=255)                                                     agaaAGGCTGGCTAGCAACTTAAAGCAAGTGGGCCAAAGCGTAGAAAGCTGACATTCGTCATTTTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCGTTGTGGATGTGACCCAGGAAGTAGAGCGTCATCGCGCCGCACTg                                                                                             >  2:109232/1‑148 (MQ=255)                                                      gaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCTGAACTGga                                                                                           <  3:98239/149‑1 (MQ=255)                                                      gaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTg                                                                                             >  7:352749/1‑147 (MQ=255)                                                      gaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGga                                                                                           <  1:341194/149‑1 (MQ=255)                                                      gaaAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGga                                                                                           <  6:191605/149‑1 (MQ=255)                                                        aaGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTATCCTGGAGCTGGTTCAGCACCAGGCAGCCCTTGTTGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGgaga                                                                                         <  8:162858/149‑1 (MQ=255)                                                           gctggctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGc‑c                                                                                     <  5:103182/149‑1 (MQ=255)                                                               gctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaa                                                                                 <  3:24164/149‑1 (MQ=255)                                                               gctAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaa                                                                                 <  2:85217/149‑1 (MQ=255)                                                                 tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCCTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGgaga                                                                                         <  5:247778/140‑1 (MQ=255)                                                                 tAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCCTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGgaga                                                                                         >  6:247778/1‑140 (MQ=255)                                                                   gCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaagcaa                                                                             >  2:22822/1‑149 (MQ=255)                                                                    cAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAAc                                                                                               <  1:14828/131‑1 (MQ=255)                                                                    cAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAAc                                                                                               >  2:14828/1‑131 (MQ=255)                                                                    cAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaagcaag                                                                            >  3:83236/1‑149 (MQ=255)                                                                    cAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaagcaag                                                                            >  5:153107/1‑149 (MQ=255)                                                                    cAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaagcaag                                                                            <  7:33134/149‑1 (MQ=255)                                                                      aCTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtg                                                                          <  1:254538/149‑1 (MQ=255)                                                                       cTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtgg                                                                         <  4:83236/149‑1 (MQ=255)                                                                        ttCCAGCATGTTTGCGAAAGCGTCGATAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGGGCTTGTGGTTTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtggt                                                                        <  4:103727/149‑1 (MQ=255)                                                                        ttCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGCCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtggt                                                                        >  5:269659/1‑149 (MQ=255)                                                                          ccAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATcg                                                                                                       <  8:332759/117‑1 (MQ=255)                                                                          ccAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATcg                                                                                                       >  7:332759/1‑117 (MQ=255)                                                                          ccAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtggtg                                                                       >  5:281190/1‑148 (MQ=255)                                                                           cAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCgcgc                                                                                                    >  4:363174/1‑119 (MQ=255)                                                                           cAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCgcgc                                                                                                    <  3:363174/119‑1 (MQ=255)                                                                           cAGCAAGTGGGCGAAAGCGTCGAAAGCTGACATTCTGAATTGTCGAAAGTTAACCTGGAGCTGGGTCATCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGa                                                                      <  5:166257/148‑1 (MQ=255)                                                                                    ggCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGaa                                                            <  4:355022/149‑1 (MQ=255)                                                                                     gCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAac                                                           <  6:281190/149‑1 (MQ=255)                                                                                       gAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGCACTCAAGCAAGTGGTGAACGGCCTGAacc                                                          <  1:22822/149‑1 (MQ=255)                                                                                          aGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaa                                                                                 >  2:102727/1‑122 (MQ=255)                                                                                          aGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTcaa                                                                                 <  1:102727/122‑1 (MQ=255)                                                                                                gAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGt                                                 <  3:155050/148‑1 (MQ=255)                                                                                                 aaaGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtg                                                                          <  3:131674/122‑1 (MQ=255)                                                                                                 aaaGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAgtg                                                                          >  4:131674/1‑122 (MQ=255)                                                                                                      tGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGcc                                                                <  2:233653/127‑1 (MQ=255)                                                                                                        ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGATCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATAccc                                        <  8:352749/149‑1 (MQ=255)                                                                                                        ccATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATAccc                                        <  3:353103/149‑1 (MQ=255)                                                                                                         cATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCa                                       <  6:378701/149‑1 (MQ=255)                                                                                                         cATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGACGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAACTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCa                                       >  5:376018/1‑149 (MQ=255)                                                                                                          aTTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATAcc                                         >  5:149749/1‑146 (MQ=255)                                                                                                                 aTTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTc                                                >  7:270892/1‑132 (MQ=255)                                                                                                                 aTTGTAGCAAGTTAACCTGTAGCTGGGTAAGCACCAGGCAGCCATTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAATTGGTGAACGGCCTGAACCACCATCTGTc                                                <  8:270892/132‑1 (MQ=255)                                                                                                                     tCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACggctggc                           >  8:213361/1‑149 (MQ=255)                                                                                                                            ttAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCt                    >  2:366047/1‑149 (MQ=255)                                                                                                                             ctgccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCt                    >  6:40129/4‑148 (MQ=255)                                                                                                                               aCCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat                 <  6:286493/149‑1 (MQ=255)                                                                                                                                ccTGGTGCTGACCCAGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACACAGCAACGTCTGGCTATTGCtgat                 >  4:241667/1‑148 (MQ=255)                                                                                                                                ccTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCtgat                 >  5:16627/1‑148 (MQ=255)                                                                                                                                      gCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTa          <  6:153107/149‑1 (MQ=255)                                                                                                                                       cTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTAt         <  6:16627/149‑1 (MQ=255)                                                                                                                                          ggTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATggc      <  6:149749/149‑1 (MQ=255)                                                                                                                                             cAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGc                                                                 <  3:267606/87‑1 (MQ=255)                                                                                                                                             cAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGc                                                                 >  4:267606/1‑87 (MQ=255)                                                                                                                                              aGCTCCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGgcggcg  <  5:40129/149‑1 (MQ=255)                                                                                                                                                cACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACggctgg                            >  3:63008/1‑121 (MQ=255)                                                                                                                                                cACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACggctgg                            <  4:63008/121‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CCTTCGATGCTGAGTATGGGGTGACCCTTGAGGACCTGGCTGAGCGCTATGAAGAAAGGCTGGCTAGCAACTTCCAGCAAGTGGGCGAAAGCGTCGAAAGCTGCCATTCGGCATTGTCGCAAGTTAACCTGGAGCTGGGTCAGCACCAGGCAGCCCTTGTGGATTTGAGCCAGGAAGTCGAGCGTCATCGCGCCGAACTGGAGAGC‑CTCAAGCAAGTGGTGAACGGCCTGAACCACCATCTGTCCGATACCCAGCAACGGCTGGCTCTTGCTGATGAGCGTATGGCGGCG  >  NC_002947/1989433‑1989722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑