Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,029,111	T→C	7.2%	L284P (CTG→CCG)	wbpM →	polysaccharide biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,029,111	0	T	C	7.2%	202.2 / 4.7	69	L284P (CTG→CCG)	wbpM	polysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base C (2/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAGCAGATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCC  >  NC_002947/2028972‑2029256                                                                                                                                             |                                                                                                                                                  cagcagATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTg                                                                                                                                           <  7:204285/148‑1 (MQ=255) cagcagATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTg                                                                                                                                           >  8:204285/1‑148 (MQ=255)    cagATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCg                                                                                                                                       >  2:260422/1‑149 (MQ=255)     agATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCgg                                                                                                                                      <  1:172309/149‑1 (MQ=255)         gatCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTc                                                                                                                                   >  7:255610/1‑148 (MQ=255)          atCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCCGGCCACTTGCCAGGTcgg                                                                                                                                 <  4:337461/149‑3 (MQ=255)           tCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCa                                                                                                                                  >  1:17701/1‑147 (MQ=255)             aaGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGt                                                                                                                              >  1:279305/1‑149 (MQ=255)             aaGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGt                                                                                                                              <  2:220844/149‑1 (MQ=255)              aGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCaa                                                                                                                                              >  8:74697/1‑132 (MQ=255)              aGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCaa                                                                                                                                              <  7:74697/132‑1 (MQ=255)                gAAACCGGTGCATAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                           >  6:225852/1‑149 (MQ=255)                gAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTTCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                           <  4:10708/149‑1 (MQ=255)                gAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                           <  3:168242/149‑1 (MQ=255)                gAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTCGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                           >  6:293961/1‑149 (MQ=255)                 aaaCCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGAt                                                                                                                          <  2:103382/149‑1 (MQ=255)                       gTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATc                                                                                                                    <  4:51839/149‑1 (MQ=255)                        tGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATcc                                                                                                                   <  2:17701/149‑1 (MQ=255)                          cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATcc                                                                                                                   >  7:84614/1‑147 (MQ=255)                          cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCaa                                                                                                                 <  2:259925/149‑1 (MQ=255)                          cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCCGGCCACTTGCCAGGTcg                                                                                                                                  <  8:160732/132‑2 (MQ=255)                          cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCCGGCCACTTGCCAGGTcg                                                                                                                                  >  7:160732/1‑131 (MQ=255)                           acaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATcc                                                                                                                   >  5:257041/1‑146 (MQ=255)                           acaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAg                                                                                                                <  1:260422/149‑1 (MQ=255)                            caGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGa                                                                                                               <  1:219242/149‑1 (MQ=255)                             aGGAAATCCTCCTCGCCCTGCCTTCGGTATGGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGAc                                                                                                              >  1:34748/1‑149 (MQ=255)                                  aTCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTCGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGa                                                                                                         <  5:293961/149‑1 (MQ=255)                                    cctcctCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCaaa                                                                                                                                >  6:104078/1‑124 (MQ=255)                                    cctcctCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCaaa                                                                                                                                <  5:104078/124‑1 (MQ=255)                                      tcctcGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACAtt                                                                                                     >  5:335969/1‑149 (MQ=255)                                        ctcGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTg                                                                                                    <  6:324755/148‑1 (MQ=255)                                           gCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGcc                                                                                                  <  8:255610/147‑1 (MQ=255)                                                  cTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCtt                                                                                         <  6:335969/149‑1 (MQ=255)                                                   ttCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTg                                                                                        >  6:118287/1‑149 (MQ=255)                                                      ggTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGc                                                                                     <  4:176932/149‑1 (MQ=255)                                                        tATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGt                                                                                   >  7:348142/1‑149 (MQ=255)                                                        tATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGt                                                                                   <  8:84614/149‑1 (MQ=255)                                                            gcgcgcCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGAc                                                                                <  5:225852/148‑1 (MQ=255)                                                                   cGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTAcc                                                                        <  6:257041/149‑1 (MQ=255)                                                                         aaGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaa                                                                  <  2:279305/149‑1 (MQ=255)                                                                            gagaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGAc                                                                                >  4:252834/1‑132 (MQ=255)                                                                            gagaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGAc                                                                                <  3:252834/132‑1 (MQ=255)                                                                            gagaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaagg                                                                >  5:8759/1‑148 (MQ=255)                                                                             agaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaaggaa                                                              <  8:348142/149‑1 (MQ=255)                                                                                ttCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAggg                                                           <  5:118287/149‑1 (MQ=255)                                                                                 tctcGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGgc                                                          >  2:58791/1‑149 (MQ=255)                                                                                     gCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGCAGTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGgctgc                                                       >  1:252731/1‑149 (MQ=255)                                                                                     gCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGgctgct                                                      >  4:202535/1‑149 (MQ=255)                                                                                                aCCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCa                                           >  8:300305/1‑149 (MQ=255)                                                                                                   ttACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATc                                         >  7:56318/1‑148 (MQ=255)                                                                                                    tACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACAt                                                                                                                     >  8:255714/1‑71 (MQ=255)                                                                                                    tACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACAt                                                                                                                     <  7:255714/71‑1 (MQ=255)                                                                                                     aCCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCca                                       >  2:44651/1‑148 (MQ=255)                                                                                                         gTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGc                                  <  2:34748/149‑1 (MQ=255)                                                                                                              aCGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaagg                                                                <  5:264183/114‑1 (MQ=255)                                                                                                              aCGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaagg                                                                >  6:264183/1‑114 (MQ=255)                                                                                                              aCGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACg                             <  5:78392/149‑1 (MQ=255)                                                                                                                  gcgcAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaaggaag                                                             <  2:300575/113‑1 (MQ=255)                                                                                                                              cGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCggg              >  1:24211/1‑149 (MQ=255)                                                                                                                                  cTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTTCCCGCCAAGGAAGGGCTGCTCGACCGCTGCGTCCACAAGCAAACGGTACTGGTCACCGGGGGCg          >  4:245891/1‑149 (MQ=255)                                                                                                                                   tttGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCgg         >  2:351738/1‑149 (MQ=255)                                                                                                                                   tttGCCGACCTGGAAAGTGGCCGCGTCAAAGTGTATGAAATCCAATACGAGGACATTGCCCATATCCTTGTGCGTGACACTGTAAAAGACAAGTAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTAGTCACCTGTGccta         >  4:50123/1‑147 (MQ=255)                                                                                                                                    actttgACCCGGCCACTTGCCAGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTc                                                     >  3:131760/6‑104 (MQ=38)                                                                                                                                    actttgACCCGGCCACTTGCCAGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTc                                                     <  4:131760/99‑1 (MQ=38)                                                                                                                                      gCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTgg      >  1:251890/1‑149 (MQ=255)                                                                                                                                        cGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCa                   <  7:257917/134‑1 (MQ=255)                                                                                                                                        cGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCa                   >  8:257917/1‑134 (MQ=255)                                                                                                                                        cGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCt    <  6:8759/149‑1 (MQ=255)                                                                                                                                          aCCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTcc  <  1:44651/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  CAGCAGATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCC  >  NC_002947/2028972‑2029256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑