Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,029,123	G→A	7.2%	R288Q (CGG→CAG)	wbpM →	polysaccharide biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,029,123	0	G	A	7.2%	197.3 / 4.5	69	R288Q (CGG→CAG)	wbpM	polysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (30/34);  new base A (2/3);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

CAGATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCCATCGG  >  NC_002947/2028975‑2029261                                                                                                                                                      |                                                                                                                                           cagATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCg                                                                                                                                            >  2:260422/1‑149 (MQ=255)  agATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCgg                                                                                                                                           <  1:172309/149‑1 (MQ=255)      gatCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTc                                                                                                                                        >  7:255610/1‑148 (MQ=255)       atCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCCGGCCACTTGCCAGGTcgg                                                                                                                                      <  4:337461/149‑3 (MQ=255)        tCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCa                                                                                                                                       >  1:17701/1‑147 (MQ=255)          aaGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGt                                                                                                                                   <  2:220844/149‑1 (MQ=255)          aaGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGt                                                                                                                                   >  1:279305/1‑149 (MQ=255)             gAAACCGGTGCATAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                                >  6:225852/1‑149 (MQ=255)             gAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTTCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                                <  4:10708/149‑1 (MQ=255)             gAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                                <  3:168242/149‑1 (MQ=255)             gAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTCGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGa                                                                                                                                >  6:293961/1‑149 (MQ=255)              aaaCCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGAt                                                                                                                               <  2:103382/149‑1 (MQ=255)                    gTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATc                                                                                                                         <  4:51839/149‑1 (MQ=255)                     tGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATcc                                                                                                                        <  2:17701/149‑1 (MQ=255)                       cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATcc                                                                                                                        >  7:84614/1‑147 (MQ=255)                       cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCaa                                                                                                                      <  2:259925/149‑1 (MQ=255)                       cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCCGGCCACTTGCCAGGTcg                                                                                                                                       >  7:160732/1‑131 (MQ=255)                       cacaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCCGGCCACTTGCCAGGTcg                                                                                                                                       <  8:160732/132‑2 (MQ=255)                        acaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATcc                                                                                                                        >  5:257041/1‑146 (MQ=255)                        acaGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAg                                                                                                                     <  1:260422/149‑1 (MQ=255)                         caGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGa                                                                                                                    <  1:219242/149‑1 (MQ=255)                          aGGAAATCCTCCTCGCCCTGCCTTCGGTATGGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGAc                                                                                                                   >  1:34748/1‑149 (MQ=255)                               aTCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTCGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGa                                                                                                              <  5:293961/149‑1 (MQ=255)                                 cctcctCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCaaa                                                                                                                                     <  5:104078/124‑1 (MQ=255)                                 cctcctCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCaaa                                                                                                                                     >  6:104078/1‑124 (MQ=255)                                   tcctcGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACAtt                                                                                                          >  5:335969/1‑149 (MQ=255)                                     ctcGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTg                                                                                                         <  6:324755/148‑1 (MQ=255)                                        gCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGcc                                                                                                       <  8:255610/147‑1 (MQ=255)                                               cTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCtt                                                                                              <  6:335969/149‑1 (MQ=255)                                                ttCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTg                                                                                             >  6:118287/1‑149 (MQ=255)                                                   ggTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGc                                                                                          <  4:176932/149‑1 (MQ=255)                                                     tATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGt                                                                                        <  8:84614/149‑1 (MQ=255)                                                     tATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGt                                                                                        >  7:348142/1‑149 (MQ=255)                                                         gcgcgcCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGAc                                                                                     <  5:225852/148‑1 (MQ=255)                                                                cGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTAcc                                                                             <  6:257041/149‑1 (MQ=255)                                                                      aaGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaa                                                                       <  2:279305/149‑1 (MQ=255)                                                                         gagaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGAc                                                                                     <  3:252834/132‑1 (MQ=255)                                                                         gagaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGAc                                                                                     >  4:252834/1‑132 (MQ=255)                                                                         gagaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaagg                                                                     >  5:8759/1‑148 (MQ=255)                                                                          agaTTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaaggaa                                                                   <  8:348142/149‑1 (MQ=255)                                                                             ttCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAggg                                                                <  5:118287/149‑1 (MQ=255)                                                                              tctcGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGgc                                                               >  2:58791/1‑149 (MQ=255)                                                                                  gCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGCAGTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGgctgc                                                            >  1:252731/1‑149 (MQ=255)                                                                                  gCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGgctgct                                                           >  4:202535/1‑149 (MQ=255)                                                                                             aCCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCa                                                >  8:300305/1‑149 (MQ=255)                                                                                                ttACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATc                                              >  7:56318/1‑148 (MQ=255)                                                                                                 tACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACAt                                                                                                                          >  8:255714/1‑71 (MQ=255)                                                                                                 tACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACAt                                                                                                                          <  7:255714/71‑1 (MQ=255)                                                                                                  aCCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCca                                            >  2:44651/1‑148 (MQ=255)                                                                                                      gTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGc                                       <  2:34748/149‑1 (MQ=255)                                                                                                           aCGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaagg                                                                     >  6:264183/1‑114 (MQ=255)                                                                                                           aCGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaagg                                                                     <  5:264183/114‑1 (MQ=255)                                                                                                           aCGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACg                                  <  5:78392/149‑1 (MQ=255)                                                                                                               gcgcAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCaaggaag                                                                  <  2:300575/113‑1 (MQ=255)                                                                                                                           cGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCggg                   >  1:24211/1‑149 (MQ=255)                                                                                                                               cTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTTCCCGCCAAGGAAGGGCTGCTCGACCGCTGCGTCCACAAGCAAACGGTACTGGTCACCGGGGGCg               >  4:245891/1‑149 (MQ=255)                                                                                                                                tttGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCgg              >  2:351738/1‑149 (MQ=255)                                                                                                                                tttGCCGACCTGGAAAGTGGCCGCGTCAAAGTGTATGAAATCCAATACGAGGACATTGCCCATATCCTTGTGCGTGACACTGTAAAAGACAAGTAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTAGTCACCTGTGccta              >  4:50123/1‑147 (MQ=255)                                                                                                                                 actttgACCCGGCCACTTGCCAGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTc                                                          >  3:131760/6‑104 (MQ=38)                                                                                                                                 actttgACCCGGCCACTTGCCAGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTc                                                          <  4:131760/99‑1 (MQ=38)                                                                                                                                   gCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTgg           >  1:251890/1‑149 (MQ=255)                                                                                                                                     cGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCa                        <  7:257917/134‑1 (MQ=255)                                                                                                                                     cGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCa                        >  8:257917/1‑134 (MQ=255)                                                                                                                                     cGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCt         <  6:8759/149‑1 (MQ=255)                                                                                                                                       aCCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTcc       <  1:44651/149‑1 (MQ=255)                                                                                                                                           ggCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCg                                                                                  <  6:33011/70‑1 (MQ=255)                                                                                                                                           ggCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCg                                                                                  >  5:33011/1‑70 (MQ=255)                                                                                                                                            gCAAGTGGCCGGGTCAAAGTGGATGAGATCCAAGACGTGGACATTGCGGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCCCTCgg  >  6:229853/1‑149 (MQ=255)                                                                                                                                            gCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCCATc    <  7:300305/147‑1 (MQ=255)                                                                                                                                            gCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCCATCg   <  2:24211/148‑1 (MQ=255)                                                                                                                                            gCAAGTGGCCGGGTCAAAGTGGATCACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCCATc    <  3:50123/147‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                           CAGATGATCAAGGAAACCGGTGCACAGGAAATCCTCCTCGCCCTGCCTTCGGTATCGCGCGCCCGGCGCAAGGAGATTCTCGCCTTGCTCGAACCTTACCCGTTGCACGTGCGCAGC‑GTGCCGGCCTTTGCCGACCTGGCAAGTGGCCGGGTCAAAGTGGATGACATCCAAGACGTGGACATTGCCGATTTGCTTGGGCGTGACCCGGTACCCGCCAAGGAAGGGCTGCTCGAACGCTGCATCCACAAGCAAACGGTACTGGTCACCGGGGCCGGTGGCTCCATCGG  >  NC_002947/2028975‑2029261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑