Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,052,784 G→T 8.9% intergenic (+16/+34) prmB → / ← PP_1828 N5‑glutamine S‑adenosyl‑L‑methionine‑dependent methyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,052,7840GT 8.9% 168.7 / 6.9 56intergenic (+16/+34)prmB/PP_1828N5‑glutamine S‑adenosyl‑L‑methionine‑dependent methyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/23);  new base T (3/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

TGTCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTAC  >  NC_002947/2052642‑2052925
                                                                                                                                              |                                                                                                                                             
tGTCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCgg                                                                                                                                         >  2:190171/1‑149 (MQ=255)
    gAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGccc                                                                                                                                      >  8:101785/1‑148 (MQ=255)
     aGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGGTTAGGCTATTTGGGGCCGCGCAGCGGCCCt                                                                                                                                     >  4:254730/1‑148 (MQ=255)
            gTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTcc                                                                                                                              <  5:221463/148‑1 (MQ=255)
                cccGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCgg                                                                                                                         <  5:241376/149‑1 (MQ=255)
                     ggTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGAt                                                                                                                    <  1:206291/149‑1 (MQ=255)
                      gTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATg                                                                                                                   >  2:30840/1‑149 (MQ=255)
                        ggACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCg                                                                                                                 >  5:173305/1‑149 (MQ=255)
                         gACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCgg                                                                                                                <  6:142722/149‑1 (MQ=255)
                         gACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCAGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCgg                                                                                                                >  2:78932/1‑149 (MQ=255)
                           cTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTc                                                                                                              >  2:92670/1‑149 (MQ=255)
                                 ctggctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCg                                                                                                        >  7:65355/1‑149 (MQ=255)
                                    gctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCgg                                                                                                       <  1:190171/147‑1 (MQ=255)
                                    gctggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCgg                                                                                                       <  1:78932/147‑1 (MQ=255)
                                       ggAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTACCCCGGCTGATGCTGTCTAAGCGGGTAg                                                                                                   >  6:377259/1‑148 (MQ=255)
                                           ttCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATc                                                                                              >  2:35513/1‑149 (MQ=255)
                                              gAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAg                                                                                           <  6:70766/149‑1 (MQ=255)
                                              gAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGCGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCa                                                                                            <  7:101785/148‑1 (MQ=255)
                                                gcggggcgggCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGAt                                                                                         <  1:35513/149‑1 (MQ=255)
                                                         gcATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGccc                                                                                >  1:291342/1‑149 (MQ=255)
                                                             ggggTATTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCgg                                                                                                                         <  5:56980/104‑1 (MQ=255)
                                                             ggggTATTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCgg                                                                                                                         >  6:56980/1‑104 (MQ=255)
                                                               ggTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCcctgcctg                                                                          <  6:40705/149‑1 (MQ=255)
                                                                     gaTGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTTCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAaca                                                                     <  5:377259/147‑1 (MQ=255)
                                                                      aTGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCTATCCAGATCAACAGCCCTGCCTGGAACACg                                                                   <  1:30840/149‑1 (MQ=255)
                                                                            aCCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACg                                                                   >  1:375646/1‑143 (MQ=255)
                                                                            aCCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACg                                                                   <  2:375646/143‑1 (MQ=255)
                                                                                          ccgccAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGc                                                                                                                        >  5:136959/1‑76 (MQ=255)
                                                                                          ccgccAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGc                                                                                                                        <  6:136959/76‑1 (MQ=255)
                                                                                          ccgccAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACcaggcagg                                                <  6:173305/148‑1 (MQ=255)
                                                                                                gCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGt                                         >  7:17324/1‑149 (MQ=255)
                                                                                                  aTCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTg                                        >  3:64870/1‑148 (MQ=255)
                                                                                                     aGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAAc                                    >  6:305782/1‑149 (MQ=255)
                                                                                                      ggAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGccac                                                        <  8:314712/128‑1 (MQ=255)
                                                                                                      ggAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGccac                                                        >  7:314712/1‑128 (MQ=255)
                                                                                                          ttGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAAt                               <  1:92670/149‑1 (MQ=255)
                                                                                                          ttGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAAt                               <  2:291342/149‑1 (MQ=255)
                                                                                                               cAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCgg                                                                                                                         >  1:354691/1‑54 (MQ=255)
                                                                                                               cAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCgg                                                                                                                         <  2:354691/54‑1 (MQ=255)
                                                                                                               cAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATccgcc                          >  8:285954/1‑149 (MQ=255)
                                                                                                                 aGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGTCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGt                        >  3:132817/1‑149 (MQ=255)
                                                                                                                     ccTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATg                                           <  1:283607/126‑1 (MQ=255)
                                                                                                                     ccTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATg                                           >  2:283607/1‑126 (MQ=255)
                                                                                                                     ccTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTc                    >  3:244783/1‑149 (MQ=255)
                                                                                                                     ccTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTc                    >  2:187582/1‑149 (MQ=255)
                                                                                                                       tCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTcgc                  >  6:286035/1‑149 (MQ=255)
                                                                                                                       tCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTcgc                  >  2:244610/1‑149 (MQ=255)
                                                                                                                          tttAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCt               >  1:331796/1‑149 (MQ=255)
                                                                                                                                 tATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGt                                         >  5:56713/1‑116 (MQ=255)
                                                                                                                                 tATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGt                                         <  6:56713/116‑1 (MQ=255)
                                                                                                                                  aattagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCgcgc                <  2:135517/135‑1 (MQ=255)
                                                                                                                                  aattagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCgcgc                >  1:135517/6‑140 (MQ=255)
                                                                                                                                   tttGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTAttt        <  8:65355/147‑1 (MQ=255)
                                                                                                                                    ttagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTAttt        >  4:338717/4‑146 (MQ=255)
                                                                                                                                    ttagggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTAttt        <  3:338717/143‑1 (MQ=255)
                                                                                                                                       gggCCGCTTCGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTAc  >  4:241192/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                             
TGTCGAGGCGTTGTACCCCGAGGTGGACTTTGCCTGGCTGGAGTTCGAGCGGGGCGGGCATGGGGTGTTCATGCTCACCGCCGAGCAGTGCCGCCAGCATCAGGAATTGTTCAAGGCCCTCGTTTAGGCTATTTGGGGCCGCGAAGCGGCCCTAATTTCCCCCGGCTGATGCGGTCTTAGCGGGTAGCAATCCAGATCAACAGCCCTGCCTGGAACACGGCAAACGCCACCAGGCAGGTAATGGTGAAACGCAATCCGCCGTCTTCGCGCTGGTATTTGGCTAC  >  NC_002947/2052642‑2052925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑