Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,060,372	A→T	7.6%	V86D (GTC→GAC)	PP_1836 ←	ZIP family metal transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,060,372	0	A	T	7.6%	213.0 / 5.7	75	V86D (GTC→GAC)	PP_1836	ZIP family metal transporter
Reads supporting (aligned to +/- strand):  ref base A (37/32);  new base T (3/3);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

CCACGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAG  >  NC_002947/2060232‑2060520                                                                                                                                               |                                                                                                                                                     ccacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATcgcg                                                                                                                                               >  3:337968/1‑148 (MQ=255) ccacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCgcgc                                                                                                                                              <  4:63926/149‑1 (MQ=255)  cacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATcgcg                                                                                                                                               >  3:118235/1‑147 (MQ=255)  cacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATcgcg                                                                                                                                               >  4:359363/1‑147 (MQ=255)  cacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATcgcg                                                                                                                                               >  6:75811/1‑147 (MQ=255)  cacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATcgcg                                                                                                                                               >  8:17653/1‑147 (MQ=255)  cacGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCgca                                                                                                                                             <  2:81984/149‑1 (MQ=255)      gTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGcac                                                                                                                                         <  3:112025/149‑1 (MQ=255)          aCGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGCTGCGCGATGTCAACGCGC‑Gcacccag                                                                                                                                     <  3:175020/149‑4 (MQ=255)           cgcgGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCg                                                                                                                                    >  8:292661/1‑149 (MQ=255)              ggcggcCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCa                                                                                                                                 <  5:75811/149‑1 (MQ=255)                   ccGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAACACGAGCTGGCGGCCC‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACg                                                                                                                            >  8:286732/1‑149 (MQ=255)                     gggCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGgc                                                                                                                          >  4:17065/1‑149 (MQ=255)                       gCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTAACATCGCGCAGCACCACCGCCAGCACGgcgc                                                                                                                        >  6:113481/1‑149 (MQ=255)                          gctGCCCGCGAGATCACGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGcc                                                                                                                       >  6:214897/3‑147 (MQ=255)                          aTTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCa                                                                                                                     <  4:118235/149‑1 (MQ=255)                                cGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCCGCACGGCGCCCAGTGCgg                                                                                                               >  6:267894/1‑149 (MQ=255)                                  tGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTg                                                                                                             <  4:337968/149‑1 (MQ=255)                                  tGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCACGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTg                                                                                                             <  3:299123/149‑1 (MQ=255)                                       tcGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCAGGCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGgcgc                                                                                                         >  1:94511/1‑149 (MQ=255)                                       tcGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGgcgc                                                                                                         >  7:135236/1‑148 (MQ=255)                                         gcgGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCa                                                                                                       >  2:187951/1‑148 (MQ=255)                                          cgGGCAGCCGCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCaaa                                                                                                     <  8:57934/149‑1 (MQ=255)                                             gCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTg                                                                                                                  >  6:12552/1‑133 (MQ=255)                                             gCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTg                                                                                                                  <  5:12552/133‑1 (MQ=255)                                             gCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGcc                                                                                                  >  2:186119/1‑149 (MQ=255)                                                gCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCACCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGa                                                                                               >  5:150853/1‑149 (MQ=255)                                                gCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGACGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGa                                                                                               >  1:60952/1‑149 (MQ=255)                                                   tCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACaa                                                                                            >  1:354418/1‑149 (MQ=255)                                                     cAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAAcc                                                                                          >  6:146993/1‑149 (MQ=255)                                                         ccTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGcc                                                                                      <  2:186317/149‑1 (MQ=255)                                                            ggCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAg                                                                                   >  5:292318/1‑149 (MQ=255)                                                             gCAGAATCAGCGACAACGAGCTGGCGTCCA‑GCATCATGCCGGCAGCAAAGCCCAGCAGCACATCCTGGTGGC‑GCGAGTTGACATCGCGCTGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCa                                                                                    <  5:267894/147‑1 (MQ=255)                                                             gCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAg                                                                                   >  7:273716/1‑148 (MQ=255)                                                             gCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAg                                                                                   >  7:61320/1‑148 (MQ=255)                                                             gCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGc                                                                                  <  1:174006/149‑1 (MQ=255)                                                                      gCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAgg                                                                         >  4:280466/1‑149 (MQ=255)                                                                         aaaaCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTg                                                                       >  1:339829/1‑148 (MQ=255)                                                                          aaaCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTg                                                                       >  6:334014/1‑147 (MQ=255)                                                                           aaCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAg                                                                    <  3:5056/149‑1 (MQ=255)                                                                           aaCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAg                                                                    >  4:5056/1‑149 (MQ=255)                                                                           aaCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAg                                                                    <  7:360824/149‑1 (MQ=255)                                                                            aCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAgg                                                                   <  5:146993/149‑1 (MQ=255)                                                                            aCGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAgg                                                                   <  2:94511/149‑1 (MQ=255)                                                                              gAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGtt                                                                 <  3:17065/149‑1 (MQ=255)                                                                                    gcggcCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCg                                                             <  7:17653/147‑1 (MQ=255)                                                                                    gcggcCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCg                                                             <  2:60952/147‑1 (MQ=255)                                                                                          a‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGGGCAGGTTGTCGGCATGGTc                                                     <  7:286732/149‑1 (MQ=255)                                                                                            gCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTAACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCg                                                    <  5:113481/149‑1 (MQ=255)                                                                                                    ccGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCAc                                            >  3:7419/1‑149 (MQ=255)                                                                                                       gcagcaAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACcgc                                         <  8:135236/149‑1 (MQ=255)                                                                                                            aaaGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCAt                                                                                >  8:355911/1‑105 (MQ=255)                                                                                                            aaaGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCAt                                                                                <  7:355911/105‑1 (MQ=255)                                                                                                              aGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTc                                     >  6:382550/1‑146 (MQ=255)                                                                                                               gCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCaga                                 <  1:186119/149‑1 (MQ=255)                                                                                                                cccAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCagag                                >  2:80408/1‑149 (MQ=255)                                                                                                                cccAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCagag                                <  5:214897/149‑1 (MQ=255)                                                                                                                 ccAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAgg                               <  8:61320/149‑1 (MQ=255)                                                                                                                  cAGCAACACATCCTGGGTGC‑CCGCGTAGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACAGCGTTCCAGAGGc                              >  2:324729/1‑149 (MQ=255)                                                                                                                     cATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTgg                           <  8:118986/149‑1 (MQ=255)                                                                                                                       tCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTggcg                         >  4:169806/1‑149 (MQ=255)                                                                                                                        cacaTCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCa                                                                                    >  7:298716/1‑89 (MQ=255)                                                                                                                        cacaTCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCa                                                                                    <  8:298716/89‑1 (MQ=255)                                                                                                                           aTCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCAcc                                           <  2:278344/127‑1 (MQ=255)                                                                                                                           aTCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCAcc                                           >  1:278344/1‑127 (MQ=255)                                                                                                                            tCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCTACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCcag                    <  8:273716/149‑1 (MQ=255)                                                                                                                            tCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCcag                    <  5:382550/149‑1 (MQ=255)                                                                                                                              cTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCcag                    >  8:177134/1‑147 (MQ=255)                                                                                                                                   tgctgcgcgATGTCAACGCGC‑GCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCCAGCAGTACg             >  5:346154/5‑149 (MQ=255)                                                                                                                                    gctgcgcgATGTCAACGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCca                     <  6:162245/138‑1 (MQ=255)                                                                                                                                    gctgcgcgATGTCAACGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCca                     >  5:162245/4‑141 (MQ=255)                                                                                                                                    gctgcgcgATGTCAACGCGC‑GCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCcagca                  >  6:27338/4‑143 (MQ=255)                                                                                                                                    gctgcgcgATGTCAACGCGC‑GCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCcagca                  <  5:27338/140‑1 (MQ=255)                                                                                                                                          cgTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAgg                                                                   >  4:37448/1‑89 (MQ=255)                                                                                                                                          cgTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAgg                                                                   <  3:37448/89‑1 (MQ=255)                                                                                                                                               aCATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTGACGGCGTTCCAGAGGCTGGCGGCAGGCAGGACGCACACCGCcag  >  4:310451/1‑149 (MQ=255)                                                                                                                                               aCATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCcag  <  3:259759/149‑1 (MQ=255)                                                                                                                                               aCATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCca   <  4:7419/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     CCACGGTAAACGCGGCGGCCGGGCCATTGCCCGTGATCTCGCGGGCAGCCTCCAGGCCTGGCAGAATCAGCGAAAACGAGCTGGCGGCCA‑GCATCATGCCGGCAGCAAAGCCCAGCATCACATCCTGGGTGC‑GCGCGTTGACATCGCGCAGCACCACCGCCAGCACGGCGCCCAGTGCGGTGGCGCCAAAGCCCGACAACCCGCCCAGCATGGCCAGGTGCAGGTTGTCGGCATGGTCGCCGTTCACCGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAG  >  NC_002947/2060232‑2060520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑