Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,060,707 G→A 8.9% intergenic (‑79/‑48) PP_1836 ← / → PP_1837 ZIP family metal transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,060,7070GA 8.9% 126.2 / 2.5 45intergenic (‑79/‑48)PP_1836/PP_1837ZIP family metal transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/20);  new base A (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

TGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTGGCAGCGAGGTGGGCA  >  NC_002947/2060562‑2060851
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tGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATa                                                                                                                                               <  4:28006/149‑1 (MQ=255)
   ccTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCt                                                                                                                                            <  8:86463/149‑1 (MQ=255)
       ttGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGa                                                                                                                                          <  4:369885/147‑1 (MQ=255)
       ttGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACt                                                                                                                                        <  5:45502/149‑1 (MQ=255)
               aGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCtgtg                                                                                                                                >  7:64985/1‑149 (MQ=255)
                ggCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCtgtg                                                                                                                                >  3:25278/1‑148 (MQ=255)
                      cagcagTGAAGGTGGGGCGGCTCTTTGGCTATGGGTGGTTGGCATGCGAACCACGAATCAGTGATTGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCtaa                                                                                                                         <  4:62058/149‑1 (MQ=255)
                            tGAAGGTGGGGCGGCGCTTTGGCTATGGGTGCGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACAGCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCctct                                                                                                                   <  3:136753/149‑1 (MQ=255)
                                 gTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACa                                                                                                              <  8:27886/149‑1 (MQ=255)
                                        ggcgCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATc                                                                                                       <  8:64985/149‑1 (MQ=255)
                                           gcTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGa                                                                                                    >  8:315263/1‑149 (MQ=255)
                                                   tATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGa                                                                                             >  2:14038/1‑148 (MQ=255)
                                                   tATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGa                                                                                             >  5:19359/1‑148 (MQ=255)
                                                       ggtgggtgGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTa                                                                                         >  6:134759/1‑148 (MQ=255)
                                                                 aTGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCt                                                                                          <  3:87323/137‑1 (MQ=255)
                                                                 aTGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCt                                                                                          >  4:87323/1‑137 (MQ=255)
                                                                       aCCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTc                                                                        >  6:34187/1‑149 (MQ=255)
                                                                        ccTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTCCGACATCCGCCAAGGATTCCCATCTATTGGCCCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAACTCAGACATGGGATCTACCTTCAACAGCCGGGTCg                                                                       >  8:69526/1‑149 (MQ=255)
                                                                              aTCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGa                                                                 >  7:99644/1‑149 (MQ=255)
                                                                              aTCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGa                                                                 <  7:315263/149‑1 (MQ=255)
                                                                                  gTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCAt                                                             >  4:193437/1‑149 (MQ=255)
                                                                                   tGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCAtt                                                            <  8:99644/149‑1 (MQ=255)
                                                                                      attcattcagtcTACCGAGGGACCTGCGACATCCGCCAATGATTCCCTTCTTTTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCATACATTGGATCTACCTTCTACAGCCTGGTCGGGCTGATCATTCt                                                          <  7:240142/142‑1 (MQ=255)
                                                                                      aTGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCt                                                          <  5:34187/148‑1 (MQ=255)
                                                                                          agtcagtcTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGgcgc                                                     >  6:140929/1‑149 (MQ=255)
                                                                                            tcagtcTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTg                                                   >  6:201658/1‑149 (MQ=255)
                                                                                                tcTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGata                                               >  4:359041/1‑149 (MQ=255)
                                                                                                   aCCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCa                                                                                                      >  6:241187/1‑91 (MQ=255)
                                                                                                   aCCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCa                                                                                                      <  5:241187/91‑1 (MQ=255)
                                                                                                      gAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTggg                                         <  7:327953/149‑1 (MQ=255)
                                                                                                       aGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCAATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGa                                                                 >  5:324189/1‑124 (MQ=255)
                                                                                                       aGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCAATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGa                                                                 >  1:62019/1‑124 (MQ=255)
                                                                                                       aGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCAATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGa                                                                 <  6:324189/124‑1 (MQ=255)
                                                                                                       aGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCAATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGa                                                                 <  2:62019/124‑1 (MQ=255)
                                                                                                            ccTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATtc                                   <  5:201658/149‑1 (MQ=255)
                                                                                                                cGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTTTGCTGTGCATCAAACCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGTATATCTGGGCCATTCTCa                                <  3:257795/148‑1 (MQ=255)
                                                                                                                  aCATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAACCTCTGGACAGGAAAACAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGCCGCTGGATATCTGGGCCATTCACAACg                             >  7:306533/1‑149 (MQ=255)
                                                                                                                      ccGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCgg                                                                      <  4:181031/104‑1 (MQ=255)
                                                                                                                      ccGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCgg                                                                      >  3:181031/1‑104 (MQ=255)
                                                                                                                         ccAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATc                        >  7:24497/1‑147 (MQ=255)
                                                                                                                                ttagcTTCTATTGGCGCGATAGCTGACTATGATGTGCAGCAAAGCCTCTGGACATGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGGTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTgg                <  8:24497/144‑1 (MQ=255)
                                                                                                                                     ttCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTGGCAGc            >  8:361380/1‑147 (MQ=255)
                                                                                                                                      tCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTGGCAGCGAg         >  5:137279/1‑149 (MQ=255)
                                                                                                                                             gcgcGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTGGCAGCGAGGTGGGc   <  3:193437/148‑1 (MQ=255)
                                                                                                                                             gcgcGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTGGCAGCGAGGTGGGCa  >  2:12539/1‑149 (MQ=255)
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TGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTACCTTCAACAGCCTGGTCGGGCTGATCATTCTGGCGCTGGATATCTGGGCCATTCTCAACGTGATCAAGAGTGGCAGCGAGGTGGGCA  >  NC_002947/2060562‑2060851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑