Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,091,357 A→G 8.8% H228R (CAC→CGC)  deaD → ATP‑dependent DEAD‑box RNA helicase DeaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,091,3570AG 8.8% 185.9 / 7.6 68H228R (CAC→CGC) deaDATP‑dependent DEAD‑box RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base A (29/33);  new base G (3/3);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

CCCGTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCACTGAACGGC  >  NC_002947/2091213‑2091505
                                                                                                                                                |                                                                                                                                                    
cccGTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCAcgc                                                                                                                                                   <  6:182889/148‑1 (MQ=255)
cccGTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCAcgc                                                                                                                                                   <  1:370221/148‑1 (MQ=255)
cccGTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCAcg                                                                                                                                                    <  6:195581/147‑1 (MQ=255)
 ccGTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCgg                                                                                                                                                 <  7:6553/149‑1 (MQ=255)
   gTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGAc                                                                                                                                               <  2:87905/149‑1 (MQ=255)
    tCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCGCGTGGAcc                                                                                                                                              <  8:229453/149‑1 (MQ=255)
        aCCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACAGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCag                                                                                                                                            >  5:383374/1‑147 (MQ=255)
         ccGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCGCGTGGACCatcag                                                                                                                                         <  3:249053/149‑5 (MQ=255)
                gTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATc                                                                                                                                      <  8:72710/145‑1 (MQ=255)
                gTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATc                                                                                                                                      >  7:72710/1‑145 (MQ=255)
                 ttCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCgg                                                                                                                                  <  4:308131/148‑1 (MQ=255)
                      cGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGtgc                                                                                                                            >  8:357997/1‑149 (MQ=255)
                      cGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGT‑CACGCGGACCAGAAGATCCCGGCTGtgct                                                                                                                           <  8:90574/149‑1 (MQ=255)
                       gCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGtgct                                                                                                                           <  8:149902/149‑1 (MQ=255)
                       gCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGtgct                                                                                                                           >  2:308710/1‑149 (MQ=255)
                           cccTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGt                                                                                                                       >  5:185486/1‑149 (MQ=255)
                                    cgtcgATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCtgg                                                                                                                >  1:344859/1‑147 (MQ=255)
                                     gtcgATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTctgctg                                                                                                                 <  2:336829/145‑1 (MQ=255)
                                     gtcgATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTctgctg                                                                                                                 >  1:336829/1‑145 (MQ=255)
                                          tCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCtggaagtggaa                                                                                                        <  6:185486/149‑1 (MQ=255)
                                            cGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAaga                                                                                                      <  8:177797/149‑1 (MQ=255)
                                              ttCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATccc                                                                                                                                    <  3:283590/117‑1 (MQ=255)
                                              ttCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATccc                                                                                                                                    >  4:283590/1‑117 (MQ=255)
                                                    cGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGAcg                                                                                              >  1:327000/1‑149 (MQ=255)
                                                     gCCGAGCGTCACCTTCGTGAACCCAATCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGAcgc                                                                                             <  2:176874/149‑1 (MQ=255)
                                                     gCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGAcgc                                                                                             <  6:383374/149‑1 (MQ=255)
                                                                ccTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCt                                                                                  >  3:175635/1‑149 (MQ=255)
                                                                 cTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGCCCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCtt                                                                                 >  5:83402/1‑149 (MQ=255)
                                                                 cTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCgg                                                                                                                                  <  1:29655/100‑1 (MQ=255)
                                                                 cTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCgg                                                                                                                                  >  2:29655/1‑100 (MQ=255)
                                                                 cTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCtt                                                                                 <  3:227990/149‑1 (MQ=255)
                                                                    cGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTc                                                                                <  5:364172/147‑1 (MQ=255)
                                                                      tGAACCCAAGCACGGCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGc                                                                            <  7:357997/149‑1 (MQ=255)
                                                                       gAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCg                                                                           >  7:24939/1‑149 (MQ=255)
                                                                       gAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCg                                                                           <  6:83402/149‑1 (MQ=255)
                                                                                   gTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCaagcaagc                                                               >  6:120427/1‑149 (MQ=255)
                                                                                    tCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCaagcaagc                                                               >  5:189352/1‑148 (MQ=255)
                                                                                    tCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCaagcaagc                                                               >  3:181776/1‑148 (MQ=255)
                                                                                      aaGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCAc                                                            >  7:8225/1‑149 (MQ=255)
                                                                                        gATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCAccc                                                          >  6:181999/1‑149 (MQ=255)
                                                                                               aCCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCt                                                   >  7:205354/1‑149 (MQ=255)
                                                                                                 cAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACcaa                                                                     >  4:297223/1‑129 (MQ=255)
                                                                                                 cAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACcaa                                                                     <  3:297223/129‑1 (MQ=255)
                                                                                                 cAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCt                                                         <  3:124041/141‑1 (MQ=255)
                                                                                                 cAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCt                                                         >  4:124041/1‑141 (MQ=255)
                                                                                                 cAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTgg                                                 >  1:273148/1‑149 (MQ=255)
                                                                                                  aaGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGgc                                                <  6:189352/149‑1 (MQ=255)
                                                                                                    gACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGa                                                      >  7:350744/1‑141 (MQ=255)
                                                                                                    gACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGa                                                      <  8:350744/141‑1 (MQ=255)
                                                                                                       ccAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACcaagca                                                                  <  6:279179/126‑1 (MQ=255)
                                                                                                       ccAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACcaagca                                                                  >  5:279179/1‑126 (MQ=255)
                                                                                                          gACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCgcgc                                        <  5:181999/149‑1 (MQ=255)
                                                                                                                 aCCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGtgct                                                                                                                           >  7:263821/1‑59 (MQ=255)
                                                                                                                 aCCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGtgct                                                                                                                           <  8:263821/59‑1 (MQ=255)
                                                                                                                       aTCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAgg                           <  2:327000/149‑1 (MQ=255)
                                                                                                                          gAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTa                        <  5:120427/149‑1 (MQ=255)
                                                                                                                           aGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTAc                       <  1:308710/149‑1 (MQ=255)
                                                                                                                            gCAGGCCCACCTGATGGTCCACGCTGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGcc                                <  3:46423/139‑1 (MQ=255)
                                                                                                                            gCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGAGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCCCTGGAAGca                                >  4:46423/1‑138 (MQ=255)
                                                                                                                                gCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGgc                  >  8:13807/1‑149 (MQ=255)
                                                                                                                                  ccACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGgctg                >  8:351044/1‑149 (MQ=255)
                                                                                                                                       ttctGGTCCGCGTGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCcgccgc                                          >  5:147239/4‑118 (MQ=255)
                                                                                                                                       ttctGGTCCGCGTGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCcgccgc                                          <  6:147239/115‑1 (MQ=255)
                                                                                                                                       ttctGGTCCGCGTGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCa           >  7:480/4‑149 (MQ=255)
                                                                                                                                       ttctGGTCCGCGTGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCa           >  2:283335/4‑149 (MQ=255)
                                                                                                                                         aTGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCAc          <  8:24939/148‑1 (MQ=255)
                                                                                                                                          tGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCAc          <  5:68473/147‑1 (MQ=255)
                                                                                                                                          tGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCAc          >  6:68473/1‑147 (MQ=255)
                                                                                                                                                aCGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCACTGAACGGc  >  2:285413/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CCCGTCAGACCGTACTGTTCTCCGCAACCCTGCCGTCGTCGATCCGTTCGATCGCCGAGCGTCACCTGCGTGAACCCAAGCACGTCAAGATCCAGACCAAGACCCAGACCGTTACCGCGATCGAGCAGGCCCACCTGATGGTCCACGCGGACCAGAAGATCCCGGCTGTGCTGCGTCTGCTGGAAGTGGAAGAGTTCGACGCGCTGATCGCCTTCGTGCGTACCAAGCAAGCCACCCTGGACCTGGCCGCCGCGCTGGAAGCCAAAGGCTACAAGGCTGCTGCACTGAACGGC  >  NC_002947/2091213‑2091505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑